A5087086061- Bacillus and Francisella bacterial research
- Bacterial Genetics and Biotechnology
- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Bacteriophages and microbial interactions
- Ubiquitin and proteasome pathways
- Genetically Modified Organisms Research
- Enzyme Structure and Function
- Transgenic Plants and Applications
- Cancer, Hypoxia, and Metabolism
- DNA Repair Mechanisms
- Protein Structure and Dynamics
- Tuberculosis Research and Epidemiology
- Peptidase Inhibition and Analysis
- Clostridium difficile and Clostridium perfringens research
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Bacterial biofilms and quorum sensing
- bioluminescence and chemiluminescence research
- RNA and protein synthesis mechanisms
- Antimicrobial Peptides and Activities
- Cancer-related Molecular Pathways
- Cancer Cells and Metastasis
- Nuclear Structure and Function
- Hippo pathway signaling and YAP/TAZ
Northwestern University
2020-2024
Jawaharlal Nehru University
2011-2019
MRC Laboratory of Molecular Biology
2011-2014
Bacillus anthracis, the etiological agent of anthrax, is a major bioterror agent. Vaccination most effective prophylactic measure available against anthrax. Currently anthrax vaccines have issues multiple booster dose requirement, adjuvant-associated side effects and stability. Use biocompatible biodegradable nanoparticles to deliver antigens immune cells could solve associated with vaccines. We hypothesized that delivery stable immunogenic domain 4 protective antigen (PAD4) anthracis...
Abstract Surface localized microbial enolases’ binding with human plasminogen has been increasingly proven to have an important role in initial infection cycle of several pathogens. Likewise, surface Mycobacterium tuberculosis ( Mtb ) enolase also binds plasminogen, and this interaction may entail crucial consequences for granuloma stability. The current study is the first attempt explore interacting residues from . Beginning structural modeling enolase, pose was predicted using...
It is known that microtubule-binding proteins including the Ska1 complex and DNA replication licensing factor, Cdt1, enable kinetochore-localized Ndc80 to form robust kinetochore-microtubule attachments. However, it not clear how stably coupled dynamic spindle microtubule plus-ends. Here, we have developed a conditional auxin-inducible degron approach reveal function for Cdt1 in chromosome segregation kinetochore–microtubule interactions separable from its role licensing. Further,...
Abstract Castration-resistant prostate cancer (CRPC) progresses despite androgen deprivation therapy, as cells adapt to grow without testosterone, becoming more aggressive and prone metastasis. CRPC biology complicates the development of effective therapies, posing challenges for patient care. Recent gene-expression metabolomics studies highlight Hexosamine Biosynthetic Pathway (HBP) a critical player, with key components like GNPNAT1 UAP1 being downregulated in metastatic CRPC. knockdown...
Two component systems (TCSs) can be envisaged as complex molecular devices that help the bacteria to sense its environment and respond aptly. 41 TCSs are predicted in Bacillus anthracis, a potential bioterrorism agent, of which only four have been studied so far. Thus, intricate signaling network contributed by remains largely unmapped B. anthracis needs comprehensive exploration. In this study, we functionally characterized one such system composed BAS0540 (Response regulator) BAS0541...
The role of TatD DNases as DNA repair enzymes or cell death (apoptotic) nucleases is well established in prokaryotes eukaryotes. current study aims to characterize the nuclease from Bacillus anthracis (Ba TatD) and explore its key histidine catalytic residues. Ba was found be a metal-dependent, nonspecific endonuclease which could efficiently cleave double-stranded substrates. Moreover, observed thermostable up 55°C act wide pH range indicating industrial applicability. Diethyl...
The pleiotropism of the GTP-sensing transcriptional regulator CodY is evident by gamut processes that it regulates in almost all low G+C Gram-positive bacteria, including general metabolism, biosynthesis some amino acids and transport systems, nitrogen uptake, sporulation, biofilm formation, motility virulence. role virulence has been established Bacillus anthracis, top rated bioterrorism agent. In this study, we investigated biochemical attributes global regulator. Homology modeling...
Summary Many Lamin A-associated proteins (LAAP’s) that are key constituents of the nuclear envelope (NE), assemble at “core” domains chromosomes during NE reformation and mitotic exit. However, identity function chromosomal core remain ill-defined. Here, we show a distinct section domain overlaps with centromeres/kinetochores telophase. The can thus be demarcated into kinetochore proximal (KPC) on one side segregated distal (KDC) opposite side, close to central spindle. We next tested if...
The auxin-inducible degron (AID) system is a versatile tool in cell biology and genetics, enabling conditional protein regulation through auxin-induced degradation. Integrating CRISPR/Cas9 with AID expedites tagging depletion of required human mouse cells. mechanism involves interactions between receptors like TIR1 the tag fused to target protein. presence auxin triggers ubiquitination, leading proteasome-mediated We have used explore mitotic functions replication licensing CDT1. Swift CDT1...
Abstract Cdt1 is a protein critical for DNA replication licensing and well-established to be binding partner of the minichromosome maintenance (MCM) complex. has also been demonstrated have an emerging, “moonlighting” role at kinetochore via direct microtubules Ndc80 However, it not known how structure conformations could allow these multiple, completely unique sets complexes. And while there exist multiple robust methods study entirely folded or unfolded proteins, structure-function studies...
ABSTRACT Cdt1 is a mixed folded protein critical for DNA replication licensing and it also has “moonlighting” role at the kinetochore via direct binding to microtubules Ndc80 complex. However, unknown how structure conformations of could allow participate in these multiple, unique sets complexes. While robust methods exist study entirely or unfolded proteins, structure–function studies combined, folded/disordered proteins remain challenging. In this work, we employ orthogonal biophysical...
Abstract The Ndc80 kinetochore complex is essential for robust kinetochore-microtubule (k-MT) attachments during mitosis. has been shown to recruit the Ska1 kinetochores, where thought aid in k-MT coupling by Ndc80. Our previous work that Cdt1, a DNA replication licensing factor, novel mitotic spindle-associated protein also recruited kinetochores via and required stabilizing attachments. In this study, we developed auxin-induced degron (AID)-tagging validate previously demonstrated role of...