A5007482619- Toxoplasma gondii Research Studies
- Cytomegalovirus and herpesvirus research
- Autophagy in Disease and Therapy
- interferon and immune responses
- Mosquito-borne diseases and control
- Herpesvirus Infections and Treatments
- Calcium signaling and nucleotide metabolism
- Influenza Virus Research Studies
- Bacillus and Francisella bacterial research
- Cannabis and Cannabinoid Research
- Bacterial Genetics and Biotechnology
- Heme Oxygenase-1 and Carbon Monoxide
- Clostridium difficile and Clostridium perfringens research
- Enzyme Production and Characterization
- Vibrio bacteria research studies
- Molecular Biology Techniques and Applications
- Tuberculosis Research and Epidemiology
- Immune cells in cancer
- Sphingolipid Metabolism and Signaling
- Bacteriophages and microbial interactions
- Advanced Proteomics Techniques and Applications
- Antibiotic Resistance in Bacteria
Heinrich Heine University Düsseldorf
2023
Microbial Chemistry Research Foundation
2023
Washington University in St. Louis
2018-2023
Jawaharlal Nehru University
2011-2016
International Centre for Genetic Engineering and Biotechnology
2015-2016
MRC Laboratory of Molecular Biology
2010
Abstract Here we report a novel regulatory mechanism for autophagy-mediated degradation of Mycobacterium tuberculosis (Mtb) and specific strategy exploited by the virulent Mtb to evade it. We show while both avirulent (H37Ra) (H37Rv) mycobacteria could readily localize autophagosomes, their maturation into autolysosomes (flux) was significantly inhibited latter strain. The inhibition autophagy flux strain highly selective, as it did not perturb basal in macrophages. Selective Mtb- containing...
In contrast to the importance of type II interferon-γ (IFN-γ) in control toxoplasmosis, role I IFN is less clear. We demonstrate here that TgIST, a secreted effector previously implicated blocking IFN-γ signaling, also blocked IFN-β responses by inhibiting STAT1/STAT2-mediated transcription infected cells. Consistent with for cell intrinsic control, ∆ Tgis t mutants were more susceptible growth inhibition murine and human macrophages activated IFN-β. Additionally, was important production...
To define cellular immunity to the intracellular pathogen Toxoplasma gondii , we performed a genome-wide CRISPR loss-of-function screen identify genes important for (interferon gamma) IFN-γ-dependent growth restriction. We revealed role tumor suppressor NF2/Merlin maximum induction of Interferon Stimulated Genes (ISG), which are positively regulated by transcription factor IRF-1. then an ISG-targeted that identified host E3 ubiquitin ligase RNF213 as necessary IFN-γ-mediated control T. in...
Toxoplasma infections in humans and other mammals are largely controlled by IFN-γ produced the activated adaptive immune system. However, we still do not completely understand role of cell-intrinsic functions controlling or apicomplexan infections. The present work identifies intrinsic activities naive macrophages counteracting T. gondii infection. Using an avirulent strain , highlight importance Nox complexes conferring protection against parasite infection both vitro vivo . We also...
Abstract Cellular reactive oxygen species (ROS) is a major antibacterial defense mechanism used by macrophages upon activation. Exposure of Mycobacterium tuberculosis ( Mtb )-infected to hypoxia known compromise the survival pathogen. Here we report that hypoxia-induced control intracellular load in RAW 264.7 was mediated regulating cellular ROS levels. We show similar classical activation, incubation resulted decreased mitochondrial outer membrane potential (MOMP) and concomitant increase...
Toxoplasma gondii is an important human pathogen infecting estimated one in three people worldwide. The cytokine interferon gamma (IFNγ) induced during infection and critical for restricting T. growth cells. Growth restriction presumed to be due the induction of interferon-stimulated genes (ISGs) that are upregulated protect host from infection. Although there hundreds ISGs by IFNγ, their individual roles parasite cells remain somewhat elusive. To address this deficiency, we screened a...
Abstract To define novel mechanisms for cellular immunity to the intracellular pathogen Toxoplasma gondii , we performed a genome-wide CRISPR loss-of-function screen provide an unbiased assessment of genes important IFN-γ-dependent growth restriction. We revealed previously unknown role tumor suppressor NF2/Merlin maximum induction Interferon Stimulated Genes (ISG), which are positively regulated by transcription factor IRF-1. then additional focused ISG-targeted that identified host E3...
Abstract Toxoplasma gondii is an important human pathogen infecting estimated 1 in 3 people worldwide. The cytokine interferon gamma (IFNγ) induced during infection and critical for restricting T. growth cells. Growth restriction presumed to be due the induction stimulated genes (ISGs) that are upregulated protect host from infection. Although there hundreds of ISGs by IFNγ, their individual roles parasite cells remain somewhat elusive. To address this deficiency, we screened a library 414...