Sedimentation has been a standard methodology for particle size analysis since the early 1900s. In recent years laser diffraction is beginning to replace sedimentation as prefered technique in some industries, such marine sediment analysis. However, of soils, which have diverse range both and shape, still requires evaluation its reliability. this study, based sieve plummet balance method were used measure distribution 22 soil samples representing four contrasting Australian Soil Orders....

ADVERTISEMENT RETURN TO ISSUEPREVFeaturesNEXTPeer Reviewed: A Subcellular Imaging by Dynamic SIMS Ion Microscopy.By imaging isotopes, the transport of ions, molecules, and therapeutic drugs can be studies in single cells.Subhash Chandra, Duane R. Smith, George H. MorrisonCite this: Anal. Chem. 2000, 72, 3, 104 A–114 APublication Date (Web):February 1, 2000Publication History Published online1 February 2000Published inissue 1...

ABSTRACT Co-localization of the elements calcium, potassium, sodium and magnesium with sequestering organelles has been achieved by application two microscopy techniques on same cell. Organelles were first localized laser scanning confocal (LSCFM) using fluorescent organelle stains. The cells then analyzed for elemental distribution ion microscopy. This approach identified a perinuclear region prominent total calcium concentration Golgi apparatus. Live fluorescently stained C6-NBD-cera-mide...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTQuantitative imaging of boron, calcium, magnesium, potassium, and sodium distributions in cultured cells with ion microscopyWalter A. Ausserer, Yong Chien. Ling, Subhash. Chandra, George H. MorrisonCite this: Anal. Chem. 1989, 61, 24, 2690–2695Publication Date (Print):December 15, 1989Publication History Published online1 May 2002Published inissue 15 December...

SUMMARY A freeze‐fracture methodology was standardized for tissue culture cells to study intracellular distribution of diffusible elements with ion microscopy. Chinese hamster ovary (CHO) and normal rat kidney (NRK) grown on a silicon substrate were sandwiched using another smooth surface (silicon, glass, mica) in the presence spacers fast frozen liquid nitrogen slush. The sandwich fractured by prying two halves apart under nitrogen. This procedure produced large areas containing hundreds...

Field capacity (FC) and permanent wilting point (PWP) are two critical input parameters required in various biophysical models. There limited published data on FC PWP of dryland cropping soils across north-western Victoria. Direct measurements time-consuming expensive. Reliable prediction from their functional relationships with routinely measured soil properties can help to circumvent these constraints. This study provided using undisturbed samples as functions geomorphological unit, type,...

The intestinal absorption of calcium includes at least three definable steps; transfer across the microvillar membrane, movement through cytosolic compartment, and energy-dependent extrusion into lamina propria, Tracing epithelium has been hampered by lack suitable techniques and, in this study, advantage was taken ion microscopy conjunction with cryosectioning use stable isotope 44Ca to visualize transit during absorptive process. effect vitamin D, required for optimal absorption,...

Summary Boron neutron capture therapy (BNCT) of cancer depends on the selective delivery a sufficient number boron‐10 ( 10 B) atoms to individual tumour cells. Cell killing results from B (n, α) 7 Li and fission reactions that occur if are localized in Intranuclear localization enhances efficiency cell via damage DNA. The net cellular content reflects both bound free pools boron assessment these pools, delivered by agent, currently cannot be made at subcellular‐scale resolution clinically...

Abstract The purpose of the present study was to evaluate both in vitro and vivo a series boron-containing nucleosides that potentially could be used as delivery agents for neutron capture therapy. rationale their synthesis based on fact proliferating neoplastic cells have increased requirements nucleic acid precursors, and, therefore, they should preferentially localize tumor. A 3-carboranlyalkyl thymidine analogs has been synthesized subset, designated N4, N5, N7, corresponding...

An ion microscope has been used to demonstrate that the calcium is present in amyloplasts of root-cap cells corn, pea, and lettuce. The localization gravity-sensing organelle suggests a possible role mechanism plant roots.

Ultrasensitive detection of nitroexplosives is accomplished by utilizing the fluorescent probe dihydro-oxoisobenzofuranyl-phthalazinone obtained from Cd( ii )-catalyzed cyclization azinodimethylidyne-benzoic acid.

The transport of K+, Na+, and Ca2+ were imaged in individual cells with a Cameca IMS-3f ion microscope. Strict cryogenic frozen freeze-dry sample preparations employed. Ion redistribution artifacts conventional chemical are discussed. Cryogenically prepared freeze-fractured freeze-dried cultured allowed the three-dimensional microscopic imaging elements. As smaller structures calcium images can be resolved 0.5 μm spatial resolution, correlative techniques needed to confirm their identity....

SUMMARY SIMS matrix effects (mass interferences, sputter yield variations and practical ion variations) were evaluated in freeze‐fractured, freeze‐dried cultured cells at the ∼0.5 μm spatial resolution of Cameca IMS‐3f microscope. Cell lines studied include normal rat kidney (NRK), 3T3 mouse fibroblast, L6 myoblast, chinese hamster ovary (CHO) kangaroo (PtK2) cells. High mass studies indicated that secondary signals H — , C O Na + Mg CN P S Cl K Ca free from major interferences. However, a...

Summary Boron measurements at subcellular scale are essential in boron neutron capture therapy (BNCT) of cancer as the nuclear localization boron‐10 atoms can enhance effectiveness killing individual tumour cells. Since tumours contain a heterogeneous population cells interphase well M phase (mitotic division) cell cycle, it is important to evaluate distribution both phases. In this work, secondary ion mass spectrometry (SIMS) based imaging technique microscopy was used quantitatively image...

Spatial variability in grain yield can arise from variation many different soil and terrain properties. Identification of important sources that bear significant relationship with help achieve more effective site-specific management. This study had three aims: (i) a geostatistical description/modelling the paddock-level spatial structure plant-available water capacity (PAWC) related properties, (ii) to determine optimal number management zones paddock, (iii) assess if PAWC properties is...