A5015105551- Pregnancy and preeclampsia studies
- Phagocytosis and Immune Regulation
- Chronic Myeloid Leukemia Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Reproductive System and Pregnancy
- Chronic Lymphocytic Leukemia Research
- Prenatal Screening and Diagnostics
- Effects of Radiation Exposure
- DNA Repair Mechanisms
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Immune Response and Inflammation
- Cancer therapeutics and mechanisms
- Cancer Genomics and Diagnostics
- Blood disorders and treatments
- Birth, Development, and Health
- Autism Spectrum Disorder Research
- Renal and related cancers
- Child Development and Digital Technology
- Neonatal and Maternal Infections
- Immune cells in cancer
- Parvovirus B19 Infection Studies
- Assisted Reproductive Technology and Twin Pregnancy
- Galectins and Cancer Biology
- Radiation Therapy and Dosimetry
- Protein Tyrosine Phosphatases
University Hospital of Basel
2018-2024
University of Basel
2019-2024
University of Bern
2023-2024
University Hospital of Bern
2023-2024
Comenius University Bratislava
2015-2023
Cancer Research Institute of the Slovak Academy of Sciences
2015-2020
Slovak Academy of Sciences
2015-2020
Biomedical Research Center of the Slovak Academy of Sciences
2016
Abstract Ionizing radiation induced foci (IRIF) are considered the most sensitive indicator for DNA double‐strand break (DSB) detection. Monitoring DSB induction by low doses of ionizing is important due to increasing exposure in general population. γH2AX and 53BP1 commonly used molecular markers situ IRIF assessment. Imaging flow cytometry (IFC) via ImageStream system provides a new opportunity this field. We analyzed formation 53BP1, their co‐localization γ‐rays (2, 5, 10, 50, 200 cGy)...
Termed as galectin-13, placental protein 13 (PP13) is exclusively expressed in the placenta of anthropoid primates. Research on PP13 normal and pathologic pregnancies show alteration concentrations pregnancy affected by preeclampsia or gestational diabetes. Galectins are also described potent immunomodulators, regulates T cell function placenta. Therefore, this study aims to investigate effects neutrophils; a type often ignored pregnancy, but present uterus from early stages pregnancy. Since...
Several recent studies have shown that liver injury is associated with the release of DNA from hepatocytes. This stimulates innate immunity and induces sterile inflammation, exacerbating damage. Similar mechanisms been described for acute renal injury. Deoxyribonuclease degrades cell-free can potentially prevent some induced tissue study analyzed effects thioacetamide-induced hepatorenal on plasma in rats. Plasma both nuclear mitochondrial origin was higher thioacetamide-treated animals....
Gestational diabetes mellitus is a transient form of glucose intolerance occurring during pregnancy. Pregnancies affected by gestational are at risk for the development preeclampsia, severe life threatening condition, associated with significant feto-maternal morbidity and mortality. It factor long-term health in women their offspring. Pregnancy has been shown to be subliminal degree neutrophil activation tightly regulated generation extracellular traps (NETs). This response excessive cases...
Ionizing radiation-induced foci (IRIF) known also as DNA repair represent the most sensitive and specific assay for assessing double-strand break (DSB). IRIF are usually visualized enumerated with aid of fluorescence microscopy using antibodies to phosphorylated γH2AX 53BP1. Although several approaches software packages were developed quantification IRIF, not one them was commonly accepted inter-laboratory variability in outputs reported. In this study, JCountPro validated enumeration two...
Introduction Autism spectrum disorders (ASD) and hyperactivity symptoms exhibit an incidence that is male-biased. Thus androgen activity can be considered a plausible biological risk factor for these disorders. However, there insufficient information about the association between increased in children with ASD. Methods In present study, relationship parameters of androgenicity (plasmatic testosterone levels receptor sensitivity) 60 boys (age 3–15) ASD investigated. Given well documented...
Extracellular DNA (ecDNA) is studied as a possible biomarker, but also trigger of the immune responses important for pathogenesis several diseases. deoxyribonuclease (DNase) activity cleaves ecDNA. The aim our study was to describe interindividual variability ecDNA and DNase in plasma healthy mice, analyze potential determinants variability, including sex, age, bodyweight. In this experiment, 58 adult CD1 mice (41 females 31 males) variable age (3 16 months old) bodyweight (females 25.7 52.1...
G-CSF for stem cell mobilisation increases circulating levels of myeloid cells at different stages maturation. Polymorphonuclear (PMNs) are also mobilised in high numbers. It was previously reported that primes PMNs towards the release neutrophils extracellular traps (NETs). Since NETs often involved thrombotic events, we hypothesised blood concentrations could enhance PMN priming NET formation healthy hematopoietic donors, predisposing them to events. However, found does not prime toward...
Abstract Purpose: Myeloproliferative neoplasms (MPN) dysregulate JAK2 signaling. Because clinical inhibitors have limited disease-modifying effects, type II such as CHZ868 stabilizing inactive and reducing MPN clones, gain interest. We studied whether cells escape from ll inhibition. Experimental Design: were continuously exposed to CHZ868. used phosphoproteomic analyses ATAC/RNA sequencing characterize acquired resistance inhibition, targeted candidate mediators in mice. Results: showed...
<div>AbstractPurpose:<p>Myeloproliferative neoplasms (MPN) dysregulate JAK2 signaling. Because clinical inhibitors have limited disease-modifying effects, type II such as CHZ868 stabilizing inactive and reducing MPN clones, gain interest. We studied whether cells escape from ll inhibition.</p>Experimental Design:<p>MPN were continuously exposed to CHZ868. used phosphoproteomic analyses ATAC/RNA sequencing characterize acquired resistance inhibition, targeted candidate...
<p>Codilupi et al, Supplementary figures and methods</p>
<p>Codilupi et al, Supplementary figures and methods</p>
<div>AbstractPurpose:<p>Myeloproliferative neoplasms (MPN) dysregulate JAK2 signaling. Because clinical inhibitors have limited disease-modifying effects, type II such as CHZ868 stabilizing inactive and reducing MPN clones, gain interest. We studied whether cells escape from ll inhibition.</p>Experimental Design:<p>MPN were continuously exposed to CHZ868. used phosphoproteomic analyses ATAC/RNA sequencing characterize acquired resistance inhibition, targeted candidate...
Background: Myeloproliferative neoplasms (MPN) are hematologic malignancies that present with excessive production of mature myeloid blood cells. MPN characterized by somatic mutations in JAK2, CALR or MPL, which result constitutive activation JAK2 signaling including STAT3/5, MAPK and PI3K/Akt pathways. inhibitors like ruxolitinib effectively inhibit the JAK-STAT axis, but via pathway remains activated vivo, thus limiting therapeutic effects. SHP2, Gab1 Grb2 have been implicated signal...