A5055689195- DNA Repair Mechanisms
- Effects of Radiation Exposure
- Acute Lymphoblastic Leukemia research
- Carcinogens and Genotoxicity Assessment
- Childhood Cancer Survivors' Quality of Life
- Wireless Body Area Networks
- Electromagnetic Fields and Biological Effects
- Acute Myeloid Leukemia Research
- Chronic Myeloid Leukemia Treatments
- Hematopoietic Stem Cell Transplantation
- RNA Interference and Gene Delivery
- Radiation Therapy and Dosimetry
- Radiation Effects and Dosimetry
- Prenatal Screening and Diagnostics
- Radiation Dose and Imaging
- Noise Effects and Management
- Molecular Biology Techniques and Applications
- Chronic Lymphocytic Leukemia Research
- Cancer-related Molecular Pathways
- Cancer Genomics and Diagnostics
- Health, Environment, Cognitive Aging
- MicroRNA in disease regulation
- CAR-T cell therapy research
- Cell death mechanisms and regulation
- bioluminescence and chemiluminescence research
Biomedical Research Center of the Slovak Academy of Sciences
2016-2025
Cancer Research Institute of the Slovak Academy of Sciences
2016-2025
Slovak Academy of Sciences
2017-2025
Purpose: Ionizing radiation induced foci (IRIF) known also as DNA repair represent most sensitive endpoint for assessing double strand breaks (DSB). IRIF are usually visualized and enumerated with the aid of fluorescence microscopy using antibodies to γH2AX 53BP1. This study analyzed effect low dose ionizing on residual in human lymphocytes aim potential biodosimetry possible extrapolation high-dose γH2AX/53BP1 effects doses compared kinetics DSB IRIF. We whether DNaseI, which is used...
Abstract Preleukemic stem cells (PSC) containing preleukemic fusion genes (PFG) arise prenatally and represent the initial stage of acute lymphoblastic leukemia (ALL) development. Despite widespread efforts, cell origin PFG is still unclear. For first time, in order to identify immunophenotype PSCs, different subpopulations hematopoietic progenitor (HSPC) umbilical cord blood (UCB) from ALL pediatric patients control healthy children were sorted analyzed for presence diagnostically-relevant...
Despite widely accepted notion that many childhood leukemias are likely developed from hematopoietic stem/progenitor cells (HSPC) with pre-leukemic fusion genes (PFG) formed in embryonic/fetal development, the data on PFG incidence newborns contradictive. To provide a better understanding of prenatal origin leukemia, umbilical cord blood 500 was screened for presence most frequent associated pediatric B-cell acute lymphoblastic leukemia. This screening revealed relatively high ETV6-RUNX1,...
Ionizing radiation-induced foci (IRIF) known also as DNA repair represent the most sensitive and specific assay for assessing double-strand break (DSB). IRIF are usually visualized enumerated with aid of fluorescence microscopy using antibodies to phosphorylated γH2AX 53BP1. Although several approaches software packages were developed quantification IRIF, not one them was commonly accepted inter-laboratory variability in outputs reported. In this study, JCountPro validated enumeration two...
// Matus Durdik 1 , Pavol Kosik Jana Kruzliakova Lukas Jakl Eva Markova and Igor Belyaev Laboratory of Radiobiology, Cancer Research Institute, Biomedical Center, Slovak Academy Sciences, Bratislava, Slovakia Correspondence to: Belyaev, email: Igor.Beliaev@savba.sk Keywords: imaging flow cytometry, imagestream, apoptosis, γH2AX Received: December 22, 2016 Accepted: March 15, 2017 Published: ABSTRACT Hematopoietic stem/progenitor CD34+ cells (HSPC) give rise to all types blood represent a key...
Human exposure to radiofrequency electromagnetic fields (RF-EMF) is restricted prevent thermal effects in the tissue. However, at very low intensity "non-thermal" biological effects, like oxidative stress, DNA or chromosomal aberrations, etc. collectively termed genomic-instability can occur after few hours. Little known about chronic (years long) with non-thermal RF-EMF. We identified two neighboring housing estates a rural region residents exposed either relatively (control-group) high...
About 5% of patients undergoing radiotherapy (RT) develop RT-related side effects. To assess individual radiosensitivity, we collected peripheral blood from breast cancer before, during and after the RT, γH2AX/53BP1 foci, apoptosis, chromosomal aberrations (CAs) micronuclei (MN) were analyzed correlated with healthy tissue effects assessed by RTOG/EORTC criteria. The results showed a significantly higher level foci before RT in radiosensitive (RS) comparison to normal responding (NOR)....
Interventional radiologists are chronically exposed to low-dose ionizing radiation (IR), which may represent a health risk. The aim of the present study was evaluate genomic instability by analyzing chromosomal aberrations, micronuclei, and 53BP1 DNA repair foci in peripheral blood lymphocytes radiologists. Based on IAEA guidelines biodosimetry using dicentrics, average protracted whole-body dose were estimated. Since preleukemic fusion genes (PFG) primary events leading leukemia, we also...
Human exposure to radiofrequency electromagnetic fields (RF-EMF) is usually restricted by guidelines prevent thermal effects in the tissue. However, at very low intensities "non-thermal" biological effects, like oxidative stress, DNA or chromosomal aberrations, etc. collectively termed genomic-instability are possible. Little known about chronic (years long) with non-thermal RF-EMF. We identified two adjacent housing estates a rural region residents exposed either relatively (control-group)...
DNA double strand breaks (DSB) induced by ionizing radiation (IR) are usually measured using γH2AX/53BP1 repair foci, that is considered to be the most sensitive assay for DSB analysis. While fluorescence microscopy (FM) gold standard this analysis, imaging flow cytometry (IFC) may offer number of advantages such as lack background, higher cells analyzed, and sensitivity in detection damage IR at low doses. Along with appearance γH2AX variable fraction exhibits homogeneously stained signal...
The BCR/ABL preleukemic fusion gene (PFG) is one of the most frequent genes in acute lymphoblastic leukemia (ALL) and was also detected hematopoietic cells from umbilical cord blood (UCB) healthy newborns.Since stem/progenitor (HSPC) are considered to be a critical cellular target for origination leukemia, we have studied presence PFG expanded subpopulations HSPC differentiated UCB those newborns, who previously been tested positive by screening their mononuclear using RT-qPCR FISH...
Although breast cancer (BC) patients benefit from radiotherapy (RT), some radiosensitive (RS) suffer side effects caused by ionizing radiation in healthy tissues. It is thought that RS underlaid a deficiency the repair of DNA double-strand breaks (DSB). proteins such as p53-binding protein 1 (53BP1) and phosphorylated histone H2AX (γH2AX), form foci at DSB locations thus serve biomarkers. Peripheral blood lymphocytes (PBL) are commonly believed to be an appropriate cell system for assessment...
Abstract Background BCR/ABL preleukemic fusion gene (PFG) is one of the most frequent genes in acute lymphoblastic leukemia (ALL) and was also detected hematopoietic cells from umbilical cord blood (UCB) healthy newborns. Since stem/progenitor (HSPC) are considered to be a critical cellular target for origination leukemia, we have studied presence PFG expanded subpopulations HSPC differentiated UCB those newborns, who previously been tested positive by screening their mononuclear using...