Alexandra Kolenová
A5079879218- Acute Lymphoblastic Leukemia research
- Acute Myeloid Leukemia Research
- Childhood Cancer Survivors' Quality of Life
- Chronic Myeloid Leukemia Treatments
- Neutropenia and Cancer Infections
- Neuroblastoma Research and Treatments
- Hematopoietic Stem Cell Transplantation
- Ethics and Legal Issues in Pediatric Healthcare
- DNA Repair Mechanisms
- Pneumonia and Respiratory Infections
- Bacterial Infections and Vaccines
- Fungal Infections and Studies
- Cancer Genomics and Diagnostics
- Lymphoma Diagnosis and Treatment
- CAR-T cell therapy research
- Antifungal resistance and susceptibility
- Gut microbiota and health
- Pancreatic and Hepatic Oncology Research
- Histiocytic Disorders and Treatments
- Lung Cancer Research Studies
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Blood properties and coagulation
- Genomics and Rare Diseases
- Neurofibromatosis and Schwannoma Cases
- Sarcoma Diagnosis and Treatment
Comenius University Bratislava
2016-2025
National Institute of Cardiovascular Diseases
2022-2024
University Medical Center Freiburg
2022-2023
Uniwersytecki Szpital Dziecięcy
2009-2023
Children's Clinical University Hospital
2009-2023
University Hospital Bratislava
2012-2023
Essa Pharma (United States)
2022
Weatherford College
2022
Detská Fakultná Nemocnica s Poliklinikou
2017-2020
National Institute of Oncology
2012
Chromosomal rearrangements of the human MLL (mixed lineage leukemia) gene are associated with high-risk infant, pediatric, adult and therapy-induced acute leukemias. We used long-distance inverse-polymerase chain reaction to characterize chromosomal rearrangement individual leukemia patients. present data molecular characterization 1590 MLL-rearranged biopsy samples obtained from The precise localization genomic breakpoints within involved translocation partner genes (TPGs) were determined...
PURPOSE Off-label use of vemurafenib (VMF) to treat BRAF V600E mutation–positive, refractory, childhood Langerhans cell histiocytosis (LCH) was evaluated. PATIENTS AND METHODS Fifty-four patients from 12 countries took VMF 20 mg/kg/d. They were classified according risk organ involvement: liver, spleen, and/or blood cytopenia. The main evaluation criteria adverse events (Common Terminology Criteria for Adverse Events [version 4.3]) and therapeutic responses Disease Activity Score. RESULTS...
The International Berlin-Frankfurt-Münster (BFM) study group conducted a on pediatric acute lymphoblastic leukemia (ALL). Minimal residual disease (MRD) was assessed using flow cytometry (FCM), and the impact of early intensification methotrexate (MTX) dose survival evaluated.We included 6,187 patients younger than 19 years. MRD by FCM refined risk definition previously used in ALL intercontinental-BFM 2002 basis age, WBC count, unfavorable genetic aberrations, treatment response measured...
Abstract Alterations in kinase genes such as NTRK1/2/3 , RET and BRAF underlie infantile fibrosarcoma (IFS), the emerging entity ‘ NTRK ‐rearranged spindle cell neoplasms’ included latest WHO classification, a growing set of tumors with overlapping clinical pathological features. In this study, we conducted comprehensive clinicopathological molecular analysis 22 cases IFS other gene‐altered neoplasms affecting both pediatric adult patients. Follow‐up periods for 16 patients ranged length...
Recently, we described B-cell precursor acute lymphoblastic leukemia (BCP-ALL) subtype with early switch to the monocytic lineage and loss of immunophenotype, including CD19 expression. Thus far, genetic background has remained unknown. Among 726 children consecutively diagnosed BCP-ALL, 8% patients experienced detectable by flow cytometry (FC). Using exome RNA sequencing, was found positively correlate three different subtypes: PAX5-P80R mutation (5 cases out 5), rearranged DUX4 (DUX4r; 30...
Pleuropulmonary blastoma (PPB) is a rare cancer occurring mainly during early childhood and often associated with germline DICER1 mutations. It classified by the macroscopic appearance into three interrelated clinico-pathologic entities on developmental continuum. Complete tumor resection main prognostic factor can be performed at diagnosis or after neoadjuvant treatment that includes chemotherapy in some cases radiotherapy. Optimal modalities of neo- adjuvant treatments challenging taking...
Single-agent vemurafenib leads to a rapid and sustained clinical response in severe multisystem LCH but does not eradicate the disease.Longitudinal assessment of BRAF V600E during treatment shows that remission can occur despite significant amounts mutated BRAF.
Bacterial infection is the most common complication in paediatric oncological patients during cancer treatment.A suitable tool for early prediction of unfavourable course still needed.We performed a prospective longitudinal observational study to evaluate role serum biomarkers (C-reactive protein, procalcitonin, interleukin-6, presepsin) diagnosis bacteraemia (gram-negative versus gram-positive) with haematological malignancies.We observed 69 febrile episodes 33 (17 male, 16 female;...
Children with Down syndrome (DS) are at a significantly higher risk of developing acute myeloid leukemia, also termed leukemia associated DS (ML-DS). In contrast to the highly favorable prognosis primary ML-DS, limited data that available for children who relapse or have refractory ML-DS (r/r ML-DS) suggest dismal prognosis. There few clinical trials and no standardized treatment approach this population. We conducted retrospective analysis international study groups pediatric oncology...
Pediatric central nervous system (CNS) tumors represent 20-25% of childhood malignancies, with 35-40 new cases annually in Slovakia. Despite treatment advances, high mortality and poor quality life a lot persist. This study assesses the clinical features, modalities, survival rates pediatric CNS tumor patients single largest center A retrospective analysis was conducted on from January 1, 2000, to December 31, 2020, at Department Oncology Hematology National Institute Children's Diseases...
Abstract Background Effectiveness of L-asparaginase administration in acute lymphoblastic leukemia treatment is mirrored the overall outcome patients. Generally, patients differ their sensitivity to L-asparaginase; however, mechanism underlying inter-individual differences still not fully understood. We have previously shown that rewires biosynthetic and bioenergetic pathways cells activate both anti-leukemic pro-survival processes. Herein, we investigated relationship between metabolic...
Abstract The gut microbiota of paediatric oncology patients undergoing a conditioning regimen before hematopoietic stem cell transplantation is recently considered to play role in febrile neutropenia. Disruption commensal and evolution opportune pathogens community carrying plethora antibiotic-resistance genes crucial role. However, the impact, predictive association patient´s resistome course therapy still be elucidated. We analysed composition 18 transplantation, including 12 developing...
Summary This prospective multicentre trial evaluated the safety and efficacy of a thiotepa/melphalan‐based reduced intensity conditioning (RIC) haematopoietic stem cell transplantation (HSCT) in children adolescents with chronic myeloid leukaemia (CML) phase (CP). Thirty‐two patients were transplanted from matched siblings or unrelated donors. In 22 patients, HSCT was performed due to insufficient molecular response loss first‐ second‐generation tyrosine kinase inhibitor (TKI), pretransplant...
Abstract Preleukemic stem cells (PSC) containing preleukemic fusion genes (PFG) arise prenatally and represent the initial stage of acute lymphoblastic leukemia (ALL) development. Despite widespread efforts, cell origin PFG is still unclear. For first time, in order to identify immunophenotype PSCs, different subpopulations hematopoietic progenitor (HSPC) umbilical cord blood (UCB) from ALL pediatric patients control healthy children were sorted analyzed for presence diagnostically-relevant...
Abstract Monitoring of minimal residual disease (MRD) after hematopoietic stem cell transplantation (HSCT) in patients with acute lymphoblastic leukemia (ALL) is vital for timely therapeutic intervention planning. However, interpreting low‐positive results from the current standard method, quantitative PCR (qPCR) immunoglobulin and T‐cell receptor gene rearrangements (IG/TR), poses challenges due to risk false positivity caused by non‐specific amplification. We aimed improve MRD detection...
Abstract Pancreatoblastoma (PBL) is a rare malignant epithelial neoplasm that affects typically young children. Signs related to advanced upper‐abdominal tumor accompanied by elevated serum α‐fetoprotein levels in child suggest PBL, however histopathological confirmation mandatory. The mainstay of the treatment complete surgical resection. Unresectable and/or metastatic PBL may become amenable delayed surgery after neoadjuvant chemotherapy. This manuscript presents international consensus...
Gut microbial dysbiosis persists months after intensive cancer treatment in children and adolescents. This prospective study compared the intestinal microbiome of 1-3 years completion Berlin-Frankfurt-Münster protocol (BFM)-based pediatric ALL (PALL) healthy controls. To induce a favorable shift bacterial composition intestines PALL with gut disruptions, 8 weeks physical activity probiotic consumption were used. Blood analyses 16S rRNA sequencing for performed on 16 cases Significant...