A5026939464- Acute Myeloid Leukemia Research
- Acute Lymphoblastic Leukemia research
- Hematopoietic Stem Cell Transplantation
- Protein Degradation and Inhibitors
- Childhood Cancer Survivors' Quality of Life
- Neutropenia and Cancer Infections
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Histone Deacetylase Inhibitors Research
- Chronic Myeloid Leukemia Treatments
- Pluripotent Stem Cells Research
- Blood disorders and treatments
- Neuroblastoma Research and Treatments
- Biomedical Ethics and Regulation
- Hematological disorders and diagnostics
- Viral-associated cancers and disorders
- Fungal Infections and Studies
- Sepsis Diagnosis and Treatment
- Neurofibromatosis and Schwannoma Cases
- Chronic Lymphocytic Leukemia Research
- Virus-based gene therapy research
- Ethics and Legal Issues in Pediatric Healthcare
- Antibiotics Pharmacokinetics and Efficacy
- Genomics and Rare Diseases
- Histiocytic Disorders and Treatments
Centre Hospitalier Universitaire Sainte-Justine
2015-2024
Université de Montréal
2014-2024
Institute for Research in Immunology and Cancer
2004-2019
Hôpital Maisonneuve-Rosemont
2018-2019
St. John's University
2013
The determinants of normal and leukemic stem cell self-renewal remain poorly characterized. We report that expression the reactive oxygen species (ROS) scavenger glutathione peroxidase 3 (GPx3) positively correlates with frequency leukemia cells (LSCs) in Hoxa9+Meis1-induced leukemias. Compared a low LSCs, high LSCs showed hypomethylation Gpx3 promoter region, expressed levels ROS. hematopoietic (HSCs) engineered to express short hairpin RNA (shRNA) were much less competitive vivo than...
Abstract The molecular hallmark of childhood acute lymphoblastic leukemia (ALL) is characterized by recurrent, prognostic genetic alterations, many which are cryptic conventional cytogenetics. RNA sequencing (RNA-seq) a powerful next-generation technology that can simultaneously identify gene rearrangements, sequence mutations and expression profiles in single assay. We examined the feasibility utility incorporating RNA-seq into prospective multicenter phase 3 clinical trial for children...
Background. Infection continues to be a major problem for children with acute myeloid leukemia (AML). Objectives were identify factors associated infection, sepsis, and infectious deaths in newly diagnosed AML.
<h3>Importance</h3> Little progress in pediatric cancer treatment has been noted the past decade, urging development of novel therapeutic strategies for adolescents and children with hard-to-treat cancers. Use comprehensive molecular profiling clinical management appears a suitable approach to improve patient care outcomes, particularly cases. <h3>Objective</h3> To assess feasibility identifying potentially actionable mutations using next-generation sequencing–based assays clinically...
Prompt recognition of a child with cancer predisposition syndrome (CPS) has implications for management, surveillance, genetic counseling, and cascade testing relatives. Diagnosis CPS requires practitioner expertise, access to testing, test result interpretation. This diagnostic process is not accessible in all institutions worldwide, leading missed diagnoses. Advances electronic health technology can facilitate risk assessment.To evaluate the accuracy prediction tool (McGill Interactive...
Acute megakaryoblastic leukemia (AMKL) is a rare, developmentally restricted, and highly lethal cancer of early childhood. The paucity hypocellularity (due to myelofibrosis) primary patient samples hamper the discovery cell- genotype-specific treatments. AMKL driven by mutually exclusive chimeric fusion oncogenes in two-thirds cases, with CBFA2T3::GLIS2 (CG2) NUP98 fusions (NUP98r) representing highest-fatality subgroups. We established CD34+ cord blood-derived CG2 models (n = 6) that...
Children with Down syndrome (DS) are at a significantly higher risk of developing acute myeloid leukemia, also termed leukemia associated DS (ML-DS). In contrast to the highly favorable prognosis primary ML-DS, limited data that available for children who relapse or have refractory ML-DS (r/r ML-DS) suggest dismal prognosis. There few clinical trials and no standardized treatment approach this population. We conducted retrospective analysis international study groups pediatric oncology...
Viridans group streptococci (VGS) cause significant morbidity in children treated for acute myeloid leukemia (AML). Our goals were to determine the occurrence and impact of these infections AML understand factors that increase risk VGS viridans streptococcal shock syndrome (VSSS) this population.We conducted a retrospective, population-based cohort study included ≤18 years age with de novo at 15 Canadian centers. We evaluated related infection VSSS.Among 341 AML, occurred 78 (22.9%) over...
Summary Invasive fungal infections ( IFIs ) are a major cause of morbidity and mortality in paediatric acute myeloid leukaemia AML ). This study describes risk factors for IFI ‐related sepsis this population. We conducted population‐based, retrospective cohort children with Canada. s during chemotherapy prior to haematopoietic stem cell transplantation, relapse, persistent disease or death were identified. Risk proven probable examined. Among courses complicated by , also evaluated. There...
Key Points Engineered human models of high-fatality pediatric leukemia are relevant to uncover disease biomarkers and therapeutic vulnerabilities. NUP98-KDM5A–associated AMKL expresses SELP, MPIG6B, NEO1 is sensitive pharmacologic inhibition with ruxolitinib.
The incidence of refractory acute myeloid leukemia (AML) is on the increase due in part to an aging population that fails respond traditional therapies. High throughput genomic analysis promises better diagnosis, prognosis, and therapeutic intervention based improved patient stratification. Relevant preclinical models are urgently required advance drug development this area. collaborating oncogenes, HOXA9 MEIS1, frequently co-overexpressed cytogenetically normal AML (CN-AML), a conditional...
Relapsed/refractory acute lymphoblastic leukemia (ALL) is a leading cause of death by cancer in children. Our institution has switched relapse treatment strategy to improve survival. We reviewed records first relapse/refractory childhood ALL between 1996 and 2012. Based on length remission, site immunophenotype, patients were classified into two groups: standard-risk (SRR) high-risk refractory (HRRR). Before 2007, all uniformly treated with the same induction as at presentation, followed...
Abstract The advent of large scale genomic sequencing technologies significantly improved the molecular classification acute megakaryoblastic leukaemia (AMKL). AMKL represents a subset (∼10%) high fatality pediatric myeloid leukemia (AML). Recurrent and mutually exclusive chimeric gene fusions associated with are found in 60%‐70% cases include RBM15‐MKL1 , CBFA2T3‐GLIS2 NUP98‐KDM5A MLL rearrangements. In addition, another 4% harbor NUP98 rearrangements ( r), yet undetermined fusion partners....
Abstract Infant acute lymphoblastic leukemias (ALL) are rare hematological malignancies occurring in children younger than 1 year of age, most frequently associated with KMT2A rearrangements ( ‐r). The smaller subset without ‐r, which represents 20% infant ALL cases, is poorly characterized. Here we report two cases chemotherapy‐sensitive non‐ ‐r ALL. Transcriptome analyses revealed identical ACIN1‐NUTM1 gene fusions both derived from cryptic chromosomal undetected by standard cytogenetic...
The prevalence and severity of Clostridium difficile infection (CDI) has increased over time in adult patients, but little is known about CDI pediatric cancer. primary objectives were to describe the incidence characteristics children with de novo acute myeloid leukemia (AML). secondary objective was factors associated CDI.We performed a multicenter, retrospective cohort study AML evaluated CDI. Recurrence, sepsis infection-related death examined. Factors also evaluated.Forty-three occurred...
Pilocytic astrocytoma (PA) is emerging as a tumor entity with dysregulated RAS/RAF/MEK/ERK signaling. In this study, we report the identification of novel recurrent BRAF insertion (p.V504_R506dup) in five PA cases harboring exclusively somatic tandem duplication. This alteration leads to an addition three amino acids kinase domain and has functional impact on activating MAPK phosphorylation. Importantly, show that mutation confers resistance RAF inhibitors without changing effectiveness...
A1 Role of fibrocytes in allergic rhinitis Marie-Ève Côté, Boulay, Sophie Plante, Jamila Chakir, Louis-Philippe Boulet A2 Patterns aeroallergens sensitization Northern Alberta Hanan Ahmed, Maria-Beatriz Ospina, Kyriaki Sideri, Harissios Vliagoftis A3 Addressing acceptable risk for adolescents with Food-Induced Anaphylaxis (FIA) Sara F. Johnson, Roberta L. Woodgate A4 Outcomes matched related and unrelated bone marrow transplantation after reduced-toxicity conditioning children suffering from...