A5015491880- Amyotrophic Lateral Sclerosis Research
- Virus-based gene therapy research
- Neurogenetic and Muscular Disorders Research
- Nerve injury and regeneration
- Parkinson's Disease Mechanisms and Treatments
- RNA Interference and Gene Delivery
- Alzheimer's disease research and treatments
- Neuroscience and Neuropharmacology Research
- Prion Diseases and Protein Misfolding
- Nuclear Receptors and Signaling
- RNA regulation and disease
- Advanced Fluorescence Microscopy Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- CRISPR and Genetic Engineering
- Pesticide Exposure and Toxicity
- Neuroscience and Neural Engineering
- Memory and Neural Mechanisms
- Poisoning and overdose treatments
- Neurogenesis and neuroplasticity mechanisms
- Autism Spectrum Disorder Research
- Forensic Toxicology and Drug Analysis
- SARS-CoV-2 detection and testing
- Autophagy in Disease and Therapy
- Pluripotent Stem Cells Research
- Muscle Physiology and Disorders
Louisiana State University Health Sciences Center Shreveport
2011-2023
Louisiana State University
2018
Louisiana State University Health Sciences Center New Orleans
2006-2017
Louisiana State University in Shreveport
2015
University of Colorado Health
2005
Adeno-associated virus (AAV) serotype 8 appears to be the strongest of natural serotypes reported date for gene transfer in liver and muscle. In this study, we evaluated AAV8 brain by several methods, including biophotonic imaging green fluorescent protein (GFP). adult rat hippocampus, levels GFP expressed were clearly greater with than AAV2 or AAV5 Western blot slightly but significantly AAV1 blot. substantia nigra, expression conferred was toxic dopamine neurons, although toxicity could...
We compared adeno-associated virus (AAV) serotypes for expression levels of green fluorescent protein (GFP) in the adult rat hippocampus by biophotonic imaging. Preparations AAV 8, 9, Rh10, and Rh43 incorporating cytomegalovirus (CMV) promoter-driven GFP were purified a CsCl method. Neither Rh10 nor produced greater than AAV8, which was used as reference. For AAV9, there an increase relative to AAV8. The CsCl-purified AAV8 displayed astroglial transduction pattern contrast expected neuronal...
Abstract Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) are progressive neurodegenerative disorders marked in most cases by the nuclear exclusion cytoplasmic deposition of RNA binding protein TDP43. We previously demonstrated that ALS–associated mutant TDP43 accumulates within cytoplasm, mislocalization predicts neurodegeneration. Here, we sought to prevent neurodegeneration ALS/FTD models using selective inhibitor export (SINE) compounds target exportin-1 (XPO1). SINE...
Widespread genetic modification of cells in the central nervous system (CNS) with a viral vector has become possible and increasingly more efficient. We previously applied an AAV9 cytomegalovirus/chicken beta-actin (CBA) hybrid promoter achieved wide-scale CNS transduction neonatal adult rats. However, this method transduces variety tissues addition to CNS. Thus we studied intravenous gene transfer synapsin better target neurons. noted systematic comparisons that drives lower level...
Since the discovery of neuropathological lesions made TDP-43 and ubiquitin proteins in cases frontotemporal lobar degeneration (FTLD) amyotrophic lateral sclerosis (ALS), there is a burst effort on finding related familial mutations developing animal models. We used an adeno-associated virus (AAV) vector for human expression targeted to substantia nigra (SN) rats. Though was expressed mainly neuronal nuclei as expected, it also cytoplasm, dotted along plasma membrane neurons. Cytoplasmic...
Improved spread of transduction in the central nervous system (CNS) was achieved from intravenous administration adeno-associated virus serotype-9 (AAV9) to neonatal rats. Spinal lower motor neuron efficiency estimated be 78% using highest vector dose tested at a 12-week interval. The widespread expression could aid studying diseases that affect both spinal cord and brain, such as amyotrophic lateral sclerosis (ALS). protein most relevant neuropathology ALS is transactive response...
AAV9 has emerged as an efficient adeno-associated virus (AAV) serotype for gene transfer to the central nervous system. We have used this technique study aspects of amyotrophic lateral sclerosis (ALS) by administering AAV encoding ALS-related transactive response DNA binding protein 43 kDa (TDP-43) neonatal rats. However, inducing expression in adult subjects would be preferable mimic onset symptoms ALS. expressed either green fluorescent (GFP) or TDP-43 rats after intravenous (i.v.) route...
Abstract Neurodegenerative diseases involving neurofibrillary tangle pathology are pernicious. By expressing the microtubule‐associated protein tau, a major component of tangles, with viral vector, we induce neuropathological sequelae in rats that similar to those seen human tauopathies. We tested several variants adeno‐associated virus (AAV) vector for tau expression nigrostriatal system order develop models graded onset and completeness. Whereas previous studies AAV2 vectors produced...
Pathological inclusions containing transactive response DNA-binding protein 43 kDa (TDP-43) are common in several neurodegenerative diseases including amyotrophic lateral sclerosis (ALS). TDP-43 normally localizes predominantly to the nucleus, but during disease progression, it mislocalizes cytoplasm. We expressed rats by an adeno-associated virus (AAV9) gene transfer method that transduces neurons throughout central nervous system (CNS). To mimic aberrant cytoplasmic found disease, we a...
Salivary glands are highly susceptible to radiation, and patients with head neck cancer treated radiotherapy invariably suffer from its distressing side effect, salivary hypofunction. The reduction in saliva disrupts oral functions, significantly impairs health. Previously, we demonstrated that adenoviral-mediated expression of Tousled-like kinase 1B (TLK1B) rat submandibular preserves function after single-dose ionizing radiation. To achieve long-term transgene for protection gland against...
Wastewater surveillance has proven to be a useful tool for evidence-based epidemiology in the fight against SARS-CoV-2 virus. It is particularly at population level where acquisition of individual test samples may time or cost-prohibitive. typically been performed wastewater treatment plants; however, this study was designed sample on local monitor spread virus among three communities with distinct social vulnerability indices Shreveport, Louisiana, located socially vulnerable region United...
One of the proteins most frequently found in neuropathological lesions is ubiquitin binding protein p62 (sequestosome 1). Post-mortem analysis a defining diagnostic marker several neurodegenerative diseases including amyotrophic lateral sclerosis and inclusion body myositis. Since functions degradation pathways autophagy, build-up p62-positive inclusions suggests defects clearance. was expressed unilaterally rat substantia nigra with an adeno-associated virus vector (AAV9) order to study...