A5052858564- Multiple Myeloma Research and Treatments
- Vitamin D Research Studies
- Selenium in Biological Systems
- Bone health and treatments
- Alkaline Phosphatase Research Studies
- Redox biology and oxidative stress
- Growth Hormone and Insulin-like Growth Factors
- Signaling Pathways in Disease
- Mesenchymal stem cell research
- Estrogen and related hormone effects
- Heterotopic Ossification and Related Conditions
- Medicinal Plant Pharmacodynamics Research
- Bone and Joint Diseases
- Computational Drug Discovery Methods
- Glutathione Transferases and Polymorphisms
- Lipid metabolism and disorders
- Biochemical and Molecular Research
- Gaussian Processes and Bayesian Inference
- Probability and Risk Models
- Musicians’ Health and Performance
- Statistical Methods and Bayesian Inference
- Nuclear Receptors and Signaling
- Cellular Mechanics and Interactions
- Chemokine receptors and signaling
- Bone Metabolism and Diseases
University of Würzburg
2007-2025
Klinik König-Ludwig Haus
2025
Ostbayerische Technische Hochschule Amberg-Weiden
2024
Friedrich-Alexander-Universität Erlangen-Nürnberg
2022
University of Vienna
2021
Orthopedic Center
2009
Universitätsklinikum Würzburg
2007
Bone marrow stromal cells (BMSCs) and other cell populations derived from mesenchymal precursors are developed for cell-based therapeutic strategies undergo cellular stress during ex vivo procedures. Reactive oxygen species (ROS) of environmental origin involved in redox signaling, cumulative damage, senescence, tumor development. Selenium-dependent (glutathione peroxidases [GPxs] thioredoxin reductases [TrxRs]) selenium-independent (superoxide dismutases [SODs] catalase [CAT]) enzyme...
Abstract During three-dimensional (3D) bioprinting, the integration of living cells into hydrogel matrices results in complex biophysicochemical interactions between viscosity, shear stress, and temperature, critically influencing structural functional integrity resulting constructs. This study delves short-term biological ramifications 3D extrusion printing telomerase-immortalized human mesenchymal stromal (hMSC-TERT) embedded bioinert hydrogels. Pluronic F127 custom-synthesized...
1,25-(OH)(2) vitamin D(3) is important for calcium homeostasis and cell differentiation. The key enzyme the activation of liver-derived 25(OH) 25-hydroxyvitamin 1alpha-hydroxylase. It expressed mainly in kidney but also peripheral tissues. A 1413-bp fragment 1alpha-hydroxylase promoter was cloned into luciferase vectors pGL2basic pGL3basic. Sequence analyses revealed four base exchanges three deletions compared with published sequence which were identically found five control persons. In...
Abstract 1,25(OH) 2 Vitamin D 3 (1,25(OH) ) and adhesion propagate monocyte differentiation. We identified the selenoprotein thioredoxin reductase (TrxR) as a new molecular target for in monocytes during this process. In THP1 monocytic leukemia cells stimulated TrxR mRNA levels 2—4‐fold by 4—8 h enhanced activity (60%) (as measured dithionitrobenzole‐assay) after 24 h, which declined below baseline 96 h. The addition of 100 nM selenite (approx. 50%) basal enzyme cells. relative stimulation...
Physical interaction of skeletal precursors with multiple myeloma cells has been shown to suppress their osteogenic potential while favoring tumor-promoting features. Although several transcriptome analyses patient-derived mesenchymal stem have displayed differences compared healthy counterparts, these insufficiently reflect the signatures mediated by tumor cell contact, vary due different methodologies, and lack results in lineage-committed precursors. To determine contact-mediated changes...
Mineralization disorders with a broad range of etiological factors represent huge challenge in dental diagnosis and therapy. Hypophosphatasia (HPP) belongs to the rare diseases affecting predominantly mineralized tissues, bones teeth, occurs due mutations ALPL gene, which encodes tissue-nonspecific alkaline phosphatase (TNAP). Here we analyzed stem cells from bone marrow (BMSCs), pulp (DPSCs) periodontal ligament (PDLSCs) absence presence efficient TNAP inhibitors. The differentiation...
Multiple myeloma involves early dissemination of malignant plasma cells across the bone marrow; however, initial steps remain unclear. Human marrow-derived mesenchymal stromal (hMSC) stimulate cell expansion (e.g., IL6) and simultaneously retain via chemokines CXCL12) adhesion factors. Hence, we hypothesized that imbalance between division retention drives dissemination. We present an in vitro model using primary hMSCs cocultured with INA-6 cells. Time-lapse microscopy revealed proliferation...
<p>Supplementary Figure 1. Principle and quantification of the V-well adhesion assay fluorescently labeled myeloma cells adapted by Weetall et al. 2001.</p><p>Supplementary 2. Validation image cytometric analysis cell cycle in four INA-6 cultures.</p><p>Supplementary 3. Cell pellets gained from V-Well Adhesion (Fig. 3).</p><p>Supplementary 4. Representative (one independent sample sets as seen Supplementary 3) curve fitting profiles generated Image...
<p>Supplementary Methods</p>
<p>Detachment of INA-6 daughter cells after cell division. <b>A–D,</b> divisions in interaction with confluent hMSCs. Seeding ratio INA-6:MSC = 4:20. <b>A,</b> Three examples dividing generating either two MA, or one MA and nMA as described G. Dashed circles mark mother (white), (blue), first position (green). Scale bar: 20 µm. <b>B,</b> Cell division MSC-adhering (MA) can yield mobile non–MSC-adhering (nMA) cell. <b>C,</b> Frequencies...
<p>Functional analysis of MSC-interacting subpopulations (<b>A</b>–<b>C</b>): Functional enrichment differentially expressed genes (from RNA-seq) using Metascape. <b>A,</b> Gene ontology (GO) cluster gene lists that are unique for MA (left) or nMA (right) INA-6. Circle nodes represent subsets input falling into similar GO term. Node size grows with the number genes. color defines a shared parent Two similarity score > 0.3 linked....
<p>Time-lapse analysis of INA-6 detachment from aggregates and hMSCs. <b>A,</b> Frequency observed that did or not lose cell(s). A total 87 were evaluated per datapoint. <b>B,</b> Example a “disseminating” aggregate growing on fluorescently (PKH26) stained hMSC (from A–D). Dashed green lines are trajectories detached cells. Scale bar = 50 µm. <b>C</b>–<b>E</b>, Quantitative assessment detachments. 45 events Seeding ratio INA-6:MSC 4:1....