A5010901339- Molecular Biology Techniques and Applications
- DNA Repair Mechanisms
- Genetic factors in colorectal cancer
- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- Evolution and Genetic Dynamics
- RNA and protein synthesis mechanisms
New York University
2023-2025
Center for Human Genetics
2023-2025
Mutations that accumulate in the human male germline with age are a major driver of genetic diversity and contribute to diseases. However, aging-related mutation rates have not been measured directly cells (sperm) at level individuals. We developed study design which we recalled 23 sperm donors prior banked samples provide new samples. The old sequential were separated by long timespans, ranging from 10 33 years. profiled these high-fidelity duplex sequencing demonstrate direct yields...
Abstract Mutations accumulate in the genome of every cell body throughout life, causing cancer and other genetic diseases 1-4 . Almost all these mosaic mutations begin as nucleotide mismatches or damage only one two strands DNA prior to becoming double-strand if unrepaired misrepaired 5 However, current sequencing technologies cannot resolve initial single-strand events. Here, we developed a single-molecule, long-read method that achieves single-molecule fidelity for single-base...