A5054430471- Cancer Genomics and Diagnostics
- DNA Repair Mechanisms
- Innovative Microfluidic and Catalytic Techniques Innovation
- Wnt/β-catenin signaling in development and cancer
- Skin Protection and Aging
- Axon Guidance and Neuronal Signaling
- Diagnosis and Treatment of Venous Diseases
- CRISPR and Genetic Engineering
- Hemodynamic Monitoring and Therapy
- Venous Thromboembolism Diagnosis and Management
- Molecular Biology Techniques and Applications
- Genetic factors in colorectal cancer
- Central Venous Catheters and Hemodialysis
- RNA and protein synthesis mechanisms
- Cardiac, Anesthesia and Surgical Outcomes
- 14-3-3 protein interactions
- Biomedical Ethics and Regulation
- Developmental Biology and Gene Regulation
- Hippo pathway signaling and YAP/TAZ
- Genomics and Phylogenetic Studies
- 3D Printing in Biomedical Research
- Hip and Femur Fractures
- Scientific Computing and Data Management
- Advanced biosensing and bioanalysis techniques
Center for Human Genetics
2022-2025
New York University
2022-2025
Northwestern University
2020-2024
York Hospital
2019
York Teaching Hospital NHS Foundation Trust
2019
INTRODUCTION: Class-defining clonal mutations occur in nearly all cases of adult low-grade gliomas and serve as critical prognostic diagnostic biomarkers. The ability to unlock tumor mutational status at the time surgery would significantly elevate standard surfgical adjuvant care for brain patients. However, rapid access molecular data during is not available, complicating accurate intraoperative diagnosis detection cell burden surgical margins. METHODS: In 50 specimens collected from 13...
Abstract Mutations accumulate in the genome of every cell body throughout life, causing cancer and other genetic diseases 1-4 . Almost all these mosaic mutations begin as nucleotide mismatches or damage only one two strands DNA prior to becoming double-strand if unrepaired misrepaired 5 However, current sequencing technologies cannot resolve initial single-strand events. Here, we developed a single-molecule, long-read method that achieves single-molecule fidelity for single-base...
Hip fracture is a procedure with high mortality and complication rates, there exists group especially at risk of these outcomes identified by their Nottingham Fracture Score (NHFS). Meta-analysis suggests possible benefit to this patient from intravascular volume optimisation. We investigated whether intraoperative fluid blood pressure optimisation improved complications in group. Patients NHFS ≥ 5 were enrolled into multicentre observer-blinded randomised control trial. allocated either...
The diagnosis and treatment of tumors often depends on molecular-genetic data. However, rapid iterative access to molecular data is not currently feasible during surgery, complicating intraoperative precluding measurement tumor cell burdens at surgical margins guide resections. To address this gap, we developed Ultra-Rapid droplet digital PCR (UR-ddPCR), which can be completed in 15 minutes from tissue result with an accuracy comparable standard ddPCR. We demonstrate UR-ddPCR assays for the...
Numerous applications in molecular biology and genomics require characterization of mutant DNA molecules present at low levels within a larger sample non-mutant DNA. This is often achieved either by selectively amplifying DNA, or sequencing all the followed computational identification However, selective amplification challenging for insertions deletions (indels). Additionally, may not be cost effective when only presence mutation needs to ascertained rather than its allelic fraction. The...