A5009497256- Plant Reproductive Biology
- Edible Oils Quality and Analysis
- Horticultural and Viticultural Research
- Plant and animal studies
- Lipid metabolism and biosynthesis
- Plant Physiology and Cultivation Studies
- Viral gastroenteritis research and epidemiology
- Cancer Genomics and Diagnostics
- Ethics in Clinical Research
- Seed Germination and Physiology
- Plant Molecular Biology Research
- Garlic and Onion Studies
- Plant biochemistry and biosynthesis
- Photosynthetic Processes and Mechanisms
- Molecular Biology Techniques and Applications
- Cancer-related molecular mechanisms research
- Health and Lifestyle Studies
- Potato Plant Research
- Circadian rhythm and melatonin
- Identification and Quantification in Food
- Research Data Management Practices
- Plant tissue culture and regeneration
- FOXO transcription factor regulation
- Aging, Health, and Disability
- Enzyme Catalysis and Immobilization
Andalusian School of Public Health
2014-2024
Biobanco del Sistema Sanitario Público de Andalucía
2019
Estación Experimental del Zaidín
2011-2016
Consejo Superior de Investigaciones Científicas
2012-2016
Instituto de Biología Molecular y Celular de Plantas
2014
Unidades Centrales Científico-Técnicas
2014
Centro de Ciencias Humanas y Sociales
2010
Abstract Melatonin is an indoleamine that synthesised from tryptophan under the control of enzymes arylalkylamine N‐acetyltransferase (AA‐NAT) and acetylserotonin methyltransferase (ASMT). inhibits colon cancer growth in both vivo vitro models; however, a precise mechanism responsible for inhibiting tumour has not been clearly described. Endothelin‐1 (ET‐1) peptide acts as survival factor cancer, inducing cell proliferation, protecting carcinoma cells apoptosis promoting angiogenesis. The...
Accumulation of reactive oxygen species (ROS) in the stigma several plant has been investigated. Four developmental stages (unopened flower buds, recently opened flowers, dehiscent anthers and flowers after fertilization) were analyzed by confocal laser scanning microscopy using ROS-specific probe DCFH2-DA. In all plants scrutinized, presence ROS stigmas was detected at higher levels during those phases considered "receptive" to pollen interaction. addition, these molecules also present...
Proteomic analysis of the stigmatic exudate Lilium longiflorum and Olea europaea led to identification 51 57 proteins, respectively, most which are described for first time in this secreted fluid. These results indicate that is an extracellular environment metabolically active, participating at least 80 different biological processes 97 molecular functions. The stigma showed a markedly catabolic profile appeared possess enzyme machinery necessary degrade large polysaccharides lipids by...
The major seed storage reserves in oilseeds are accumulated protein bodies and oil bodies, serve as an energy, carbon, nitrogen source during germination. Here, the spatio-temporal relationships between several key enzymes (phospholipase A, lipase, lipoxygenase) involved lipid mobilization cotyledon cells was analysed vitro Enzyme activities were assayed in-gel their cellular localization determined using microscopy techniques. At maturity, phospholipase A triacylglycerol lipase found...
A pollen grain contains a number of esterases, many which are released upon contact with the stigma surface. However, identity and function most these esterases remain unknown. In this work, from olive during its germination were identifided functionally characterized. The esterolytic capacity (Olea europaea) was examined using in vitro in-gel enzymatic assays different enzyme substrates. functional analysis achieved by inhibition specific inhibitors. cellular localization esterase...
During sexual reproduction, pollen performance is greatly influenced by the female tissues. The stigma exudate, i.e., extracellular secretion that covers outermost surface, has been usually regarded as a reservoir of water, secondary metabolites, cell wall precursors and compounds serve energy supply for rapid tube growth. In an attempt to identify proteins present in secretome, we performed large-scale analysis two species (Lilium longiflorum Olea europaea) following proteomic-based...
Breast cancer continues to be one of the main causes morbidity and mortality globally was leading cause death in women Spain 2020. Early diagnosis is most effective methods lower incidence rates breast cancer. The human metalloproteinases (MMP) mainly function as proteolytic enzymes degrading extracellular matrix plays important roles steps tumorigenesis. This retrospective cohort study shows immunohistochemical expression levels MMP-1, MMP-2, MMP-3, MMP-9 154 with 42 without tumor disease....
In some plants, pollen grains accumulate storage lipids that serve as energy supply during germination. Here, three enzymes involved in early steps of oil body mobilization the male gametophyte were functionally characterized for first time. The effect extracellular sugars on performance and dynamics was also analysed. Olive bodies showed phospholipase A, lipase, lipoxygenase activities their surface. Enzyme activity levels increased germination with a maximum after 3h. Removal from medium...
Abstract Background The pistil is a place where multiple interactions between cells of different types, origin, and function occur. Ca 2+ one the key signal molecules in plants animals. Despite numerous studies on signalling during pollen-pistil interactions, which constitute main topics plant physiology, dynamics flower formation are scarce. purpose this study was to analyze contents situ localization at whole-organ level olive whole course development. Results obtained results showed...
Biobanks are infrastructures essential for research involving multi-disciplinary teams and an increasing number of stakeholders. In the field personalized medicine, biobanks play a key role through provision well-characterized annotated samples protecting at same time right donors. The Andalusian Public Health System Biobank (SSPA Biobank) has implemented global information management system made up different modules that allow recording, traceability monitoring all associated with biobank...
Pollen quality is a useful parameter for agrobiological studies, which includes both the viability and capacity of pollen grain to germinate. In case olive (Olea europaea L.) pollen, such particular interest selection donor cultivars. this work we have compared several methods evaluate including: a) trypan blue staining, b) fluorochromatic reaction fluorescein diacetate, c) germination ability in vitro. results obtained indicate that two latter were most effective estimate quality, allowed...
Peripheral blood is the most promising source of RNA biomarkers for diagnostic and epidemiological studies, because presence disease prognostic information reflected in gene expression pattern. Quality used by a number different downstream applications, so selection appropriate stabilization purification method important. We have analyzed purified from 300 samples 25 donors processed two technicians using three methodologies with Tempus PaxGene tubes.The best quality sample results were...
The mission of the Andalusian Public Health System Biobank is to offer best options for biological samples human origin and associated clinical information, protecting rights citizens who donate their research. Since provides high-quality all types in a specified format, adapting preanalytical phase according requirements research, prospective collection distribution are being prioritized order contribute sustainability Biobank. Registry Donors Biomedical Research tool recruitment donors...
ISHS XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): Symposium Plant Physiology from Cell to Fruit Production System POLLEN AND PISTIL FEATURES INVOLVED IN THE REPRODUCTIVE BIOLOGY OF OLIVE (OLEA EUROPAEA L.)