A5013581031- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Molecular Biology Techniques and Applications
- Bacteriophages and microbial interactions
- Virus-based gene therapy research
- Microbial infections and disease research
- Aquaculture disease management and microbiota
- SARS-CoV-2 and COVID-19 Research
- Reproductive tract infections research
- interferon and immune responses
- Infective Endocarditis Diagnosis and Management
- Salmonella and Campylobacter epidemiology
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Vitamin D Research Studies
- Cancer Genomics and Diagnostics
- Metabolomics and Mass Spectrometry Studies
- HIV Research and Treatment
- CRISPR and Genetic Engineering
- Dermatological and COVID-19 studies
- COVID-19 and healthcare impacts
- Tryptophan and brain disorders
- RNA and protein synthesis mechanisms
- Immunodeficiency and Autoimmune Disorders
- Gene expression and cancer classification
Andalusian School of Public Health
2017-2025
Instituto de Salud Carlos III
2020
Biobanco del Sistema Sanitario Público de Andalucía
2020
Instituto de Investigación Biosanitaria de Granada
2016-2018
Universidad de Granada
2015-2018
High-quality human DNA samples and associated information of individuals are necessary for biomedical research. Biobanks act as a support infrastructure the scientific community by providing large number high-quality biological specific downstream applications. For this purpose, biobank methods sample preparation must ensure usefulness long-term functionality products obtained. Quality indicators tool to measure these parameters, purity integrity determination being those specifically used...
The development of porcine epidemic diarrhea virus (PEDV) antibody-based assays is important for detecting infected animals, confirming previous exposure, and monitoring sow herd immunity. However, the potential cross-reactivity among coronaviruses a major concern pathogen-specific assays. In this study, we used serum samples (n = 792) from pigs precisely known infection status multiplex fluorescent microbead-based immunoassay and/or enzyme-linked platform to characterize antibody response...
Establishment of continuous cell lines from human normal and tumor tissues is an extended useful methodology for molecular characterization cancer pathophysiology drug development in research laboratories. The exchange these between different labs a common practice that can compromise assays reliability due to contamination with microorganism such as mycoplasma or cells flasks experiment reproducibility reliability. Great proportions are contaminated and/or replaced by derived origin during...
Abstract The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed COVID-19 the SCOURGE study, we found 133 cases (1.42%) detectable clonal mosaicism for chromosome alterations (mCA) 226 (5.08%) acquired loss Y (LOY). Individuals mosaic events (mCA and/or LOY) showed a 54% increase risk...
Streptococcus Group B (GBS) colonization in pregnant women is the most important risk factor for newborn disease due to vertical transmission during delivery. GBS pregnancy has been implicated as a leading cause of perinatal infections. Traditionally, are screened between 35 and 37 weeks gestation. However, antenatal culture-based screening yields no information on status offers low predictive value at Numerous assays have evaluated an attempt validate fast efficient method. The aim this...
Members of family Coronaviridae cause a variety diseases in birds and mammals. Porcine hemagglutinating encephalomyelitis virus (PHEV), lesser-researched coronavirus, can infect naive pigs any age, but clinical disease is observed ≤4 weeks age. No commercial PHEV vaccines are available, neonatal protection from PHEV-associated presumably dependent on lactogenic immunity. Although subclinical infections thought to be common, ecology swine herds unknown. To begin address this gap knowledge,...
The frequency of mitochondrial DNA haplogroups (mtDNA-HG) in humans is known to be shaped by migration and repopulation. Mounting evidence indicates that mtDNA-HG are not phenotypically neutral, selection may contribute its distribution. Haplogroup H, the most abundant Europe, improved survival sepsis. Here we developed a random forest trained model for haplogroup calling using data procured from GWAS arrays. Our results reveal context SARS-CoV-2 pandemic, HV branch were found represent...
Purulent or exudative genitourinary infections are a frequent cause of consultation in primary and specialized healthcare. The objectives this study were: to determine the prevalence Trichomonas vaginalis co-infections with Candida spp. Gardnerella vaginal secretion; use multilocus sequence typing (MLST) analyse genetic diversity T. strains.The samples were submitted for analysis (n=5230) third-level hospital Granada (Southern Spain) between 2011 2014; eight strains isolated during 2015...
Among the ∼46 virus species in family Coronaviridae (2019), many of which are important pathogens humans and 6 commonly found pigs, porcine hemagglutinating encephalomyelitis remains one least researched. The present study provided a comprehensive characterization PHEV infection process immune responses using CDCD neonatal pigs.
Abstract: In the present study we have performed both a meta-analysis and an analytical exploring presence of Chlamydia pneumoniae , herpes simplex virus type 1, human 6, Toxoplasma gondii antibodies in sample 143 schizophrenic patients control subjects. The was on papers published up to April 2014. serum immunoglobulin G A by enzyme-linked immunosorbent assay test. detection microbial DNA total peripheral blood nested polymerase chain reaction. showed that: 1) C. brain are more common...
Abstract Aiming to increase the reproducibility of biomedical research results, biobanks obtain human tissues highest quality and carry out different storage methods adapted needs analytical technique be performed by researchers. However, there is much controversy little data concerning real impact stabilization on tissue quality, integrity functionality derived biomolecules. The influence four [RNA later (RNL), snap freezing (SF), using Optimal Cutting Tissue compound (SF-OCT)...
Porcine hemagglutinating encephalomyelitis virus (PHEV) is the cause of acute outbreaks vomiting and wasting disease and/or in neonatal pigs, with naïve herds particularly vulnerable to clinical episodes. PHEV infections older pigs are generally considered be subclinical, but poorly characterized refereed literature. In this study, twelve 7-week-old were oronasally inoculated 0.5 mL (1:128 HA titer) (Mengeling strain) then followed through 42 days post inoculation (dpi). Fecal oral fluid...
Peripheral blood is the most promising source of RNA biomarkers for diagnostic and epidemiological studies, because presence disease prognostic information reflected in gene expression pattern. Quality used by a number different downstream applications, so selection appropriate stabilization purification method important. We have analyzed purified from 300 samples 25 donors processed two technicians using three methodologies with Tempus PaxGene tubes.The best quality sample results were...
Cell lines are a widely used pre-clinical models for biomedical research. The accessibility and the relative simplicity of facilities necessary use cell lines, along with large number potential applications, encourage many researchers to choose this model. However, access from non-confident source or through interlaboratory exchange results in uncontrollable uncertain quality. Furthermore, possibility using as an endless resource multiple passages can contribute uncontrolled scenario, main...
Adequate hypothermic storage of human mesenchymal stem cells (hMSCs) is fundamental importance since they have been explored in several regenerative medicine initiatives. However, the actual clinical application hMSCs necessitates for long periods, a process that requires use non-toxic and efficient cryo-reagents capable maintaining high viability differentiating properties after thawing. Current cryopreservation methods are based on cryoprotectant agents (CPAs) containing dimethylsulphoxide...
Porcine deltacoronavirus (PDCoV), belonging to family Coronaviridae and genus Deltacoronavirus, is a major enteric pathogen in swine. Accurate PDCoV diagnosis relying on laboratory testing antibody detection an important approach. This study evaluated the potential of receptor-binding subunit spike protein (S1), generated using mammalian expression system, for specific via indirect enzyme-linked immunosorbent assay (ELISA). Serum samples were collected at day post-inoculation (DPI) -7 42,...
Abstract Objetive Peripheral Blood is the most promising source of RNA biomarkers for diagnostic and epidemiological studies, because presence disease prognostic information reflected in gene expression pattern. Quality used by a number different downstream applications, so selection appropriate stabilization purification method important. We have analyzed purified from 300 blood samples 25 donors processed two technicians using three methodologies with Tempus PaxGene tubes. Results The best...
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