A5009026023- HIV Research and Treatment
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Herpesvirus Infections and Treatments
- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- Mosquito-borne diseases and control
- HIV/AIDS drug development and treatment
Icahn School of Medicine at Mount Sinai
2022-2024
Introduction Antibodies against the SARS-CoV-2 spike protein are a critical immune determinant for protection virus. While virus neutralization is key function of spike-specific antibodies, antibodies also mediate Fc-dependent activities that can play role in or pathogenesis. Methods This study characterized serum antibody responses elicited after two doses heterologous adenovirus-vectored (Ad26/ Ad5) vaccines. Results Vaccine-induced binding titers and Fc-mediated functions decreased over...
The envelope (Env) glycoproteins on HIV-1 virions are the sole target of broadly neutralizing antibodies (bNAbs) and focus vaccines. However, many cross-reactive conserved epitopes often occluded virus particles, contributing to evasion humoral immunity. This study aimed identify Env that exposed/occluded particles investigate mechanisms their masking. Using a flow cytometry-based assay, three isolates, panel antibodies, we show only select epitopes, including V2i, gp120-g41 interface,...
Introduction Neutralizing antibodies (Abs) are one of the immune components required to protect against viral infections. However, developing vaccines capable eliciting neutralizing Abs effective a broad array HIV-1 isolates has been an arduous challenge. Objective This study sought test aimed induce epitopes at V1V2 apex envelope (Env). Methods Four groups rabbits received DNA vaccine expressing domain CRF01_AE A244 strain on trimeric 2J9C scaffold (V1V2-2J9C) along with protein consisting...
HIV-1 Env signal peptide (SP) is an important contributor to functions. generated from Vpu/Env encoded bicistronic mRNA such that the 5′ end of Env-N-terminus, encodes for Env-SP overlaps with 3′ Vpu. SP displays high sequence diversity, which translates into variability in Vpu sequence. This study aimed understand effect polymorphism Vpu-Env overlapping region (VEOR) on functions two vital viral proteins: and Env. We used infectious molecular clone pNL4.3-CMU06 swapped its (or VEOR) other...
The envelope glycoproteins (Env) on HIV-1 virions are the sole target of broadly neutralizing antibodies (bNAb) and focus vaccines. However, many cross-reactive conserved epitopes often occluded virus particles, contributing to evasion humoral immunity. This study aimed identify Env that exposed/occluded particles investigate mechanisms their masking. Using a flow cytometry-based assay, three isolates, panel antibodies, we show only select including V2i, gp120-g41 interface, gp41-MPER...