A5036579201- RNA Research and Splicing
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Brazilian Legal Issues
- Immune cells in cancer
- Religion and Society in Latin America
- Chromosomal and Genetic Variations
- Extracellular vesicles in disease
- Immune Response and Inflammation
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA and protein synthesis mechanisms
Universidade do Porto
2024
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2023-2024
University of Lisbon
2017-2019
Aging imposes a barrier to somatic cell reprogramming through poorly understood mechanisms. Here, we report that fibroblasts from old mice express higher levels of Zeb2, transcription factor activates epithelial-to-mesenchymal transition. Synthesis Zeb2 protein is controlled by natural antisense transcript named Zeb2-NAT. We show transfection adult with specific LNA Gapmers induces robust downregulation Zeb2-NAT transcripts and enhances the into pluripotent cells. further demonstrate...
ABSTRACT Spliceostatin A, meayamycin, and pladienolide B are small molecules that target the SF3b subunit of spliceosomal U2 nuclear ribonucleoprotein (snRNP). These compounds attracting much attention as tools to manipulate splicing for use potential anti-cancer drugs. We investigated effects these inhibitors on mRNA transport stability in human cells. Upon inhibition, unspliced pre-mRNAs accumulated nucleus, particularly within enlarged speckles. However, a fraction pre-mRNA were exported...
Long non-coding RNAs (lncRNAs) represent a substantial portion of the human transcriptome. LncRNAs present very stringent cell-type/tissue specificity being potential candidates for therapeutical applications during aging and disease. As example, targeting MALAT1, highly conserved lncRNA originally identified in metastatic non-small cell lung cancer, has shown promising results cancer regression. Nevertheless, regulation MALAT1 have not been directly addressed. Interestingly, locus is...
Aryl Hydrocarbon Receptor (AHR) ligands, upon binding, induce distinct gene expression profiles orchestrated by the AHR, leading to a spectrum of pro- or anti-inflammatory effects. In this study, we designed, synthesized and evaluated three indole-containing potential AHR ligands (FluoAHRL: AGT-4, AGT-5 AGT-6). All compounds were shown emit fluorescence in near-infrared. Their agonist activity was first predicted using silico docking studies, then confirmed luciferase reporter cell lines....
Transfer RNA fragments (tRFs) have gene silencing effects similarly to miRNAs, can be sorted into extracellular vesicles (EVs) and are emerging as potential circulating biomarkers for cancer diagnoses. We aimed at analyzing the expression of tRFs in gastric (GC) understanding their biomarkers. explored miRNA datasets from tumors normal adjacent tissues (NATs) TCGA repository, well proprietary 3D-cultured GC cell lines corresponding EVs, order identify differentially represented using MINTmap...
Transfer RNA fragments (tRFs) have gene silencing effects similarly to miRNAs, can be sorted into extracellular vesicles (EVs) and are emerging as potential circulating biomarkers for cancer diagnosis. We aimed at analyzing the expression of tRFs in gastric (GC) understanding their biomarkers. explored miRNA datasets from tumors normal adjacent tissues (NAT) TCGA repository, proprietary 3D-cultured GC cell lines corresponding EVs, order identify differentially represented using MINTmap...