A5103133336- Genetic and phenotypic traits in livestock
- Cancer-related molecular mechanisms research
- Sperm and Testicular Function
- Genetic Mapping and Diversity in Plants and Animals
- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Milk Quality and Mastitis in Dairy Cows
- Genetics and Plant Breeding
- Cancer-related gene regulation
- Microbial Metabolism and Applications
- IL-33, ST2, and ILC Pathways
- Pluripotent Stem Cells Research
- Mycobacterium research and diagnosis
- T-cell and B-cell Immunology
- Meat and Animal Product Quality
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Fatty Acid Research and Health
- Wnt/β-catenin signaling in development and cancer
- Biochemical Analysis and Sensing Techniques
- Hippo pathway signaling and YAP/TAZ
- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- RNA Research and Splicing
Shandong Academy of Agricultural Sciences
2007-2025
Ministry of Agriculture and Rural Affairs
2024
Chengdu Institute of Biology
2022-2023
Shandong Center for Disease Control and Prevention
2023
The posttranscriptional gene regulation mediated by microRNA plays an important role in the development and function of male female reproductive organs germ cells mammals, including cattle.In present study, we identified novel differentially expressed miRNAs testis ovary Holstein cattle combining Solexa sequencing with bioinformatics.In total 100 104 pre-miRNAs were testicular ovarian tissues, encoding 122 136 mature miRNAs, respectively.Of these, 6 appear to be bovine-specific.A 246 known...
Genetic improvement of milk fatty acid content traits in dairy cattle is great significance. However, chromatography-based methods to measure have several disadvantages. Thus, quick and accurate predictions various contents based on the mid-infrared spectrum (MIRS) from herd (DHI) data are essential meaningful expand amount phenotypic available. In this study, 24 kinds concentrations were measured samples 336 Holstein cows Shandong Province, China, using gas chromatography (GC) technique,...
Gram-negative bacteria are important pathogens in cattle, causing severe infectious diseases, including mastitis. Lipopolysaccharides (LPS) components of the outer membrane and crucial mediators chronic inflammation cattle. LPS modulations bovine immune responses have been studied before. However, single-cell transcriptomic chromatin accessibility analyses peripheral blood mononuclear cells (PBMCs) their to stimulation were never reported.We performed RNA sequencing (scRNA-seq) assay for...
ABSTRACT CFAP58 is a testis-enriched gene that plays an important role in the sperm flagellogenesis of humans and mice. However, effect on bull semen quality underlying molecular mechanisms involved spermatogenesis remain unknown. Here, we identified two single-nucleotide polymorphisms (rs110610797, A>G rs133760846, G>T) one indel (g.-1811_ g.-1810 ins147bp) promoter were significantly associated with bulls, including deformity rate ejaculate volume. Moreover, by generating...
Cattle body size measurements constitute the conformation traits that facilitate their production, fertility, and longevity status. Prioritizing functional variants causal genes of is essential for understanding genetic basis. In this study, we conducted single-trait multitrait GWAS 20 using imputed sequence data in 7,674 Chinese Holstein individuals identified 27 QTL regions. Leveraging these regions, performed Bayesian fine-mapping to identify 30 independent credible sets putative...
The random regression test-day model has become the most commonly adopted for routine genetic evaluations in dairy populations, which allows accurately accounting and environmental effects over lactation. objective of this study was to explore appropriate models evaluation milk yield a Holstein population with relatively small size, is common situation regional or independent breeding companies preform evaluation. Data included 419,567 records from 54,417 cows first Variance components...
ABSTRACT In order to estimate the influence of TYR and MC1R on color cattle hide, in Luxi Yellow, Bohai Black, China Holstein black‐white red‐white (20 animals each four breeds) were sequenced. The comparison among hide phenotypes revealed no sequence difference. sequences coding region three alleles ( E D , + e ), which previously reported. Furthermore, we found an important single nucleotide polymorphism at 725 position region, may help breed identification. A polymerase chain...
Abstract Objectives This study was performed in the frame of a more extensive dedicated to integrated analysis single-cell transcriptome and chromatin accessibility datasets peripheral blood mononuclear cells (PBMCs) with large-scale GWAS 45 complex traits Chinese Holstein cattle. Lipopolysaccharide (LPS) is crucial mediator chronic inflammation modulate immune responses. PBMCs include primary T B cells, natural killer (NK) monocytes (Mono), dendritic (DC). How LPS stimulates at level dairy...
The elite bull plays an extremely important role in the genetic progression of dairy cow population. previous results indicated potential positive relationship large scrotal circumference (SC) with improved semen volume, concentration, and motility. In order to improve bull’s quantity quality by selection, it is necessary estimate parameters traits their correlations other conformation such as SC that could be used for indirect selection. this study, seven (n = 66,260) nine 3,642) Holstein...
DNA methylation and gene alternative splicing drive spermatogenesis. In screening markers transcripts related to sperm motility, semen from three pairs of full-sibling Holstein bulls with high low motility was subjected reduced representation bisulphite sequencing. A total 948 DMRs were found in 874 genes (gDMRs). Approximately 89% gDMR-related harboured events, including SMAD2, KIF17, PBRM1. One DMR exon 29 PBRM1 the highest 5mC ratio found, hypermethylation this region bull motility....
MicroRNAs (miRNAs) play key roles in sperm as the regulatory factors involved fertility and subsequent early embryonic development. Bta-miR-6531 is specifically a highly enriched miRNA low-motility sperms previous study. To investigate mechanism of bta-miR-6531, 508 shared target genes bta-miR-6531 were predicted using two databases (TargetScan7 miRWalk). According to Kyoto Encyclopedia Genes Genomes (KEGG), calcium cAMP signaling pathways most genes. A dual-luciferase assay indicated that...
The current study conducted a preliminary investigation of genetic correlations in Chinese Holstein bulls to improve their semen quantity and quality by indirect selection conformation traits. results seven traits nine showed that the heritability estimates ranged from 0.24 (post-thaw motility) 0.63 (volume per ejaculation), while 0.29 (pin width) 0.80 (withers height). Phenotypic correlation between scrotal circumference (SC) concentration ejaculation (SCPE), SC total number sperm (TNS),...
Cell-mediated immune responses (CMIRs) are critical to building a robust system and reducing disease susceptibility in cattle. Long non-coding RNAs (lncRNAs) regulate various biological processes. However, the best of our knowledge, characterization functions lncRNAs their regulations on bovine CMIR have not been investigated comprehensively. In this study, experimental bulls were immunized with heat-killed preparation Candida albicans (HKCA) induce delayed-type hypersensitivity (DTH). Three...
Abstract Random regression test-day model has become the most commonly adopted for routine genetic evaluations different dairy populations, which allows accurately accounting and environmental effects at periods during lactation. The objective of this study was to explore appropriate random evaluation milk yield in Chinese Holstein population. Data included 419,567 records from 54,417 cows first Variance components breeding values were estimated using with order (first fifth order) Legendre...
Negative energy balance (NEB) in high-yielding cows during the peripartum period raises risk of postpartum diseases. High-level concentration non-esterified fatty acid (NEFA) is a good indicator excessive NEB. The current low-cost and high-throughput mid-infrared (MIR) spectroscopy method gradually applied to predict NEFA concentrations for NEB identification. objective this study was compare different pre-processing methods analysis models optimal predictions serum using milk MIR spectra....