A5102998191- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Viral Infections and Vectors
- Infective Endocarditis Diagnosis and Management
- Animal Virus Infections Studies
- interferon and immune responses
- Aortic Disease and Treatment Approaches
- Cardiac Structural Anomalies and Repair
- T-cell and Retrovirus Studies
- Geological and Geochemical Analysis
- Cardiac Valve Diseases and Treatments
- earthquake and tectonic studies
- High-pressure geophysics and materials
- Cardiac, Anesthesia and Surgical Outcomes
- Neuroscience and Neuropharmacology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Public Relations and Crisis Communication
- Histone Deacetylase Inhibitors Research
- Coenzyme Q10 studies and effects
- Infectious Aortic and Vascular Conditions
- Abdominal vascular conditions and treatments
- Virology and Viral Diseases
- Natural product bioactivities and synthesis
First Affiliated Hospital of Jinan University
2022-2025
Lanzhou Veterinary Research Institute
2016-2025
Chinese Academy of Agricultural Sciences
2016-2025
Lanzhou University
2010-2025
Thermo Fisher Scientific (China)
2024
Third Hospital of Hebei Medical University
2022
Hebei Medical University
2022
National Center for Drug Screening
2021
Shanghai Institute of Materia Medica
2021
Chinese Academy of Sciences
2014-2021
African swine fever—an acute, febrile, hemorrhagic, highly contacting, and lethal disease caused by fever virus (ASFV)—jeopardizes the global pig industry. Understanding mechanism ASFV employs to evade host defense during infection is essential for developing targeted drugs vaccines against ASFV.
ASFV is a large DNA virus that highly pathogenic in domestic pigs. How this sensed by the innate immune system as well why it so virulent remains enigmatic. In study, we show genome contains AT-rich regions are recognized DNA-directed RNA polymerase III (Pol-III), leading to viral sensor RIG-I-mediated responses. We further protein I267L inhibits Pol-III-RIG-I-mediated antiviral interacts with E3 ubiquitin ligase Riplet, disrupts Riplet-RIG-I interaction and impairs Riplet-mediated...
Currently, the deficiency in commercially available vaccines or therapeutic options against African swine fever constitutes a matter of major concern industry globally. Here, we report design and construction recombinant ASFV mutant harboring combinational deletions interferon inhibitors MGF360-9L MGF505-7R based on genotype II CN/GS/2018 strain currently circulating China.
Abstract African swine fever is one of the most serious viral diseases that affects domestic and wild pigs. The causative agent, virus (ASFV), has evolved sophisticated immune evasion mechanisms target both innate adaptive responses. However, underlying molecular have not been fully understood. Here, we report ASFV E184L protein inhibits host response via targeting stimulator IFN genes (STING)-mediated signaling pathway in human embryonic kidney HEK-293T cells porcine pulmonary alveolar...
African swine fever, one of the major viral diseases swine, poses an imminent threat to global pig industry. The high-efficient replication causative agent fever virus (ASFV) in various organs pigs greatly contributes disease. However, how ASFV manipulates cell population drive vivo remains unclear. Here, we found that spleen reveals most severe pathological manifestation with highest loads among during infection. By using single-cell-RNA-sequencing technology and multiple methods,...
Foot-and-mouth disease is a frequently occurring of cloven-hoofed animals that caused by infection with the foot-and-mouth virus (FMDV). FMDV circumvents type-I IFN response expressing proteins antagonize cellular innate immunity, such as leader protease and 3C protease. We identified structural protein VP3 negative regulator virus-triggered IFN-β signaling pathway. Expression inhibited Sendai activation regulatory factor-3 expression retinoic acid-inducible gene-I/melanoma...
Abstract Foot-and-mouth disease virus (FMDV) is the etiological agent of FMD, which affects cloven-hoofed animals. The pathophysiology FMDV has not been fully understood and evasion host innate immune system still unclear. Here, non-structural protein 3A was identified as a negative regulator virus-triggered IFN-β signaling pathway. Overexpression inhibited Sendai activation IRF3 expressions RIG-I/MDA5. Transient transfection co-immunoprecipitation experiments suggested that interacts with...
The African swine fever virus (ASFV) has caused a devastating pandemic in domestic and wild swine, causing economic losses to the global industry. Recombinant live attenuated vaccines are an attractive option for ASFV treatment. However, safe effective against still scarce, more high-quality experimental vaccine strains need be developed. In this study, we revealed that deletion of genes DP148R, DP71L, DP96R from highly virulent isolate CN/GS/2018 (ASFV-GS) substantially virulence swine....
Abstract The role of the innate immune protein LGP2 (laboratory genetics and physiology 2) in FMDV-infected cells remains unknown. Here, we demonstrate antiviral during FMDV infection. infection triggered mRNA expression but reduced expression. Overexpression suppressed replication, inflammatory response was significantly inhibited by virus-infected cells. N-terminal DExDc C-terminal regulatory domain regions were essential for LGP2-mediated activity against FMDV. Disruption RNA recognition...
Foot-and-mouth disease is a highly contagious viral of cloven-hoofed animals that caused by foot-and-mouth virus (FMDV). To replicate efficiently in vivo, FMDV has evolved methods to circumvent host antiviral defense mechanisms, including those induced interferons (IFNs). Previous research focused on the effect L(pro) and 3C(pro) type I IFNs. In this study, VP3 was found inhibit II IFN signaling pathways. The overexpression inhibited IFN-γ-triggered phosphorylation STAT1 at Tyr701 subsequent...
We previously reported that P-retigabine (P-RTG), a retigabine (RTG) analogue bearing propargyl group at the nitrogen atom in linker of RTG, displayed moderate anticonvulsant efficacy. Recently, our further efforts led to discovery HN37 (pynegabine), which demonstrated satisfactory chemical stability upon deleting ortho liable −NH2 and installing two adjacent methyl groups carbamate motif. exhibited enhanced activation potency toward neuronal Kv7 channels high vivo efficacy range...
African swine fever virus (ASFV) causes acute hemorrhagic infectious disease in pigs. The ASFV genome encodes various proteins that enable the to escape innate immunity; however, underlying mechanisms are poorly understood. present study found MGF-360-10L significantly inhibits interferon (IFN)-β-triggered STAT1/2 promoter activation and production of downstream IFN-stimulated genes (ISGs). deletion (ASFV-Δ10L) replication was impaired compared with parental CN/GS/2018 strain, more ISGs were...
African swine fever (ASF), a devastating infectious disease in swine, severely threatens the global pig farming industry. Disease control has been hampered by unavailability of vaccines. Here, we report that deletion QP509L and QP383R genes (ASFV-ΔQP509L/QP383R) from highly virulent ASF virus (ASFV) CN/GS/2018 strain results complete viral attenuation swine. Animals inoculated with ASFV-ΔQP509L/QP383R at 104 50% hemadsorbing dose (HAD50) remained clinically normal during 17-day observational...
African swine fever virus (ASFV) causes significant morbidity and mortality in pigs worldwide. The lack of vaccines or therapeutic options warrants urgent further investigation. To this aim, we developed a rationally designed live attenuated ASFV-Δ110-9L/505-7R mutant based on the highly pathogenic Genotype II ASFV CN/GS/2018 backbone by deleting 2 well-characterized interferon inhibitors MGF110-9L MGF505-7R. was slightly vitro compared to parental but tolerant genetic modifications even...
African swine fever, caused by a large icosahedral DNA virus (African fever virus, ASFV), is highly contagious disease in domestic and feral swine, thus posing significant economic threat to the global industry. Currently, there are no effective vaccines or available methods control ASFV infection. Attenuated live viruses with deleted virulence factors considered be most promising vaccine candidates; however, mechanism which these attenuated confer protection unclear. Here, we used Chinese...
This retrospective study aimed to develop a nomogram predict the risk of postoperative acute respiratory distress syndrome (ARDS) in patients with Stanford type A aortic dissection. The included who underwent surgical repair for dissection between January 2020 and December 2023. Demographic data, details, intraoperative information, outcomes were collected. Univariate logistic regression was used preliminary predictor screening, multivariate model constructed presented as nomogram....
African swine fever virus (ASFV) is the causative agent of (ASF), a severe hemorrhagic disease with mortality rate reaching 100%. Despite extensive research on ASFV mechanisms, no safe and effective vaccines or antiviral treatments have been developed. Live attenuated generated via gene deletion are considered to be highly promising. We developed novel recombinant strain by deleting MGF360-10L MGF505-7R, significantly reducing virulence in pigs. In inoculation experiment, pigs were infected...
The aim of this retrospective study was to evaluate the relationship between admission base excess and clinical outcomes in postoperative patients with aortic dissection. Clinical data were extracted from MIMIC-IV (Medical Information Mart for Intensive Care IV) database. association mortality dissection assessed using multivariate Cox regression Kaplan-Meier survival analysis. Subgroup analysis receiver operating characteristic (ROC) curve employed predictive performance in-hospital,...
ABSTRACT African swine fever virus (ASFV) poses a serious risk to wild boars and domestic pigs worldwide. Developing live‐attenuated vaccines (LAVs) containing specific gene deletions is an effective approach prevent control (ASF) epidemics. We created novel ASFV mutant, ASFV‐GS‐Δ7R/ΔI267L, with of genes MGF505‐7R I267L , using the highly pathogenic parental strain ASFV‐GS/2018 (ASFV‐GS). In vitro, viral titer ASFV‐GS‐Δ7R/ΔI267L was approximately 10 times lower than that its strain. Pigs...
Macroautophagy/autophagy and apoptosis are pivotal interconnected host cell responses to viral infection, including picornaviruses. Here, the VP3 proteins of picornaviruses were determined trigger autophagy, with autophagic flux being triggered by TP53-BAD-BAX axis. Using foot-and-mouth disease virus (FMDV) as a model system, we unraveled novel mechanism how picornavirus hijacks autophagy bolster replication enhance pathogenesis. FMDV infection induced both in vivo vitro. protein facilitated...
This study demonstrates that the deletion in PK region occurred naturally FMDV genome. The isolated O/ME-SA/PanAsia lineage with an 86-nt showed a pig-adapted characteristic could cause clinical signs swine but not bovines. Compared to wild-type strain, which possesses full infection capacity both and bovines, recombinant virus is deficient causing disease Deletion of previously reported 43 nt also led significantly decreased pathogenicity indicates novel determinant tropism virulence FMDV.
Takeda G protein-coupled receptor 5 (TGR5) is a promising target for treating metabolic syndrome and inflammatory diseases. Herein, we identified new series of betulinic acid derivatives as potent TGR5 agonists, which show remarkable activity on human (h) canine (c) but exhibit unpromising murine (m) TGR5. Species difference was also observed with many other reported agonists. Therefore, screened 29 amino acids were conserved in hTGR5 cTGR5 different mTGR5 found key acid, H88 (Y89 hTGR5),...