A5022819121- interferon and immune responses
- NF-κB Signaling Pathways
- Immune Response and Inflammation
- Viral Infections and Vectors
- RNA regulation and disease
- Inflammasome and immune disorders
- Ubiquitin and proteasome pathways
- Light effects on plants
- Mosquito-borne diseases and control
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Cytokine Signaling Pathways and Interactions
- Photosynthetic Processes and Mechanisms
- CAR-T cell therapy research
- Algal biology and biofuel production
- Immunotherapy and Immune Responses
- T-cell and Retrovirus Studies
- RNA Research and Splicing
- Viral Infections and Outbreaks Research
- IL-33, ST2, and ILC Pathways
- Disaster Response and Management
- Vascular Malformations Diagnosis and Treatment
- Macrophage Migration Inhibitory Factor
- Inflammatory Bowel Disease
- Toxoplasma gondii Research Studies
Wuhan Institute of Virology
2015-2024
Chinese Academy of Sciences
2014-2024
Emory University
2023-2024
Zhongnan Hospital of Wuhan University
2021-2023
Wuhan University
2009-2023
University of Chinese Academy of Sciences
2021
First People's Hospital of Chongqing
2013
Howard Hughes Medical Institute
2009
Huazhong University of Science and Technology
2004
IFN-stimulated gene 56 (ISG56) is one of the first identified proteins induced by viruses and type I IFNs. In this study, we ISG56 as a virus-induced protein associated with MITA, an adapter involved in virus-triggered induction Overexpression inhibited Sendai activation IRF3, NF-kappaB, IFN-beta promoter, whereas knockdown had opposite effects. Consistently, overexpression reversed cytoplasmic poly(I:C)-induced inhibition vesicular stomatitis virus (VSV) replication, VSV replication....
Ubiquitination and deubiquitination have emerged as critical post-translational regulatory mechanisms for activation or attenuation of the virus-triggered type I interferon (IFN) 2The abbreviations used are: IFNinterferonTLRToll-like receptorVSVvesicular stomatitis virusRNAiRNA interferenceSeVSendai virus.2The virus. induction pathways. In this study, we identified two deubiquitinating enzymes, OTUB1 OTUB2, negative regulators IFN induction. Overexpression OTUB2 inhibited virus-induced IRF3...
The tripartite motif (TRIM)-containing proteins are a family of that have been known to be involved in divergent biological processes, including important roles immune responses through regulating various signaling pathways. In this study, we identified member the TRIM family, TRIM8, as positive regulator tumor necrosis factor-α (TNFα) and interleukin-1β (IL-1β)–triggered NF-κB activation. Overexpression TRIM8 activated potentiated TNFα- IL-1β–induced activation NF-κB, whereas knockdown had...
African swine fever virus (ASFV) is a large DNA that highly contagious and pathogenic in domestic pigs with mortality rate up to 100%. However, how ASFV suppresses JAK-STAT1 signaling evade the immune response remains unclear. In this study, we found ASFV-encoded protein MGF-505-7R inhibited proinflammatory IFN-γ-mediated signaling. Mechanistically, was interact JAK1 JAK2 mediate their degradation. Further study indicated promoted degradation of by upregulating E3 ubiquitin ligase RNF125...
ASFV is a large DNA virus that highly pathogenic in domestic pigs. How this sensed by the innate immune system as well why it so virulent remains enigmatic. In study, we show genome contains AT-rich regions are recognized DNA-directed RNA polymerase III (Pol-III), leading to viral sensor RIG-I-mediated responses. We further protein I267L inhibits Pol-III-RIG-I-mediated antiviral interacts with E3 ubiquitin ligase Riplet, disrupts Riplet-RIG-I interaction and impairs Riplet-mediated...
The transcription factors IRF3 and NF-κB are required for the expression of many genes involved in antiviral innate immune response, including type I interferons (IFNs) proinflammatory cytokines. It is well established that TBK1 an essential kinase engaged downstream multiple pattern-recognition receptors (PRRs) to mediate phosphorylation activation, whereas precise mechanisms activation have not been fully elucidated yet. Here, we identified tripartite motif 26 (TRIM26) as important...
African swine fever virus (ASFV) is a large DNA that causes (ASF), an acute and hemorrhagic disease in pigs with lethality rates of up to 100%. To date, how ASFV efficiently suppress the innate immune response remains enigmatic. In this study, we identified cysteine protease pS273R as antagonist type I interferon (IFN). Overexpression inhibited JAK-STAT signaling triggered by IFNs. Mechanistically, interacted STAT2 recruited E3 ubiquitin ligase DCST1, resulting K48-linked polyubiquitination...
Viral infection causes activation of the transcription factors NF-κB and IRF3, which collaborate to induce type I interferons (IFNs) cellular antiviral response. The mitochondrial outer membrane protein VISA acts as a critical adapter for assembling virus-induced complex that signals IRF3 activation. Using biochemical purification approach, we identified WD repeat WDR5 VISA-associated protein. was recruited in viral dependent manner. also caused translocation from nucleus mitochondria....
Transcription factor IRF3-mediated type I interferon induction is essential for antiviral innate immunity. We identified the deSUMOylating enzyme Sentrin/SUMO-specific protease (SENP) 2 as a negative regulator of virus-triggered IFN-β induction. Overexpression SENP2 caused IRF3 deSUMOylation, K48-linked ubiquitination, and degradation, whereas depletion had opposite effects. Both SUMOylation ubiquitination occurred at lysines 70 87, these processes are competitive. The level was markedly...
Significance The transcription factor STAT3 plays pivotal roles in various physiological processes, including differentiation of Th cells. Its deregulation results serious diseases, inflammatory diseases and cancer. Understanding how activity is regulated important for deciphering the pathogenesis such diseases. In this study, we identified a protein called FAM64A, which promotes through modulating DNA-binding STAT3. Consequently, FAM64A also Th17 development colitis colitis-associated This...
STING plays central roles in the innate immune response to pathogens that contain DNA. Sensing cytoplasmic DNA by cyclic GMP-AMP synthase produces GMP-AMP, which binds and activates induces translocation from endoplasmic reticulum perinuclear microsome. However, this trafficking process has not been fully elucidated yet. In study, we identified YIPF5 as a positive regulator of trafficking. is essential for virus- or intracellular DNA-triggered production type I IFNs. Consistently, knockdown...
Abstract There are numerous mechanisms by which glioblastoma cells evade immunological detection, underscoring the need for strategic combinatorial treatments to achieve appreciable therapeutic effects. However, developing combination therapies is difficult due dose-limiting toxicities, blood-brain-barrier, and suppressive tumor microenvironment. Glioblastoma notoriously devoid of lymphocytes driven in part a paucity lymphocyte trafficking factors necessary prompt their recruitment...
Detection of viral nucleic acids by pattern recognition receptors initiates type I interferon (IFN) induction and innate antiviral response. The RIG-I-like (RLRs), including RIG-I MDA5, recognize cytoplasmic RNA in most cell types are critically involved MDA5 structurally related mediate similar signaling pathways. While the regulation activity has been extensively investigated, little is known about regulatory mechanisms activity. Here we identified ribonucleoprotein PTB-binding 1 (RAVER1)...
Viral infection triggers induction of antiviral cytokines and effectors, which are critical mediators innate immune response. It has been shown that the processing body-associated protein LSm14A is involved in cell lines but vivo evidence lacking. By generating LSm14A-deficient mice, this study, we show plays a specific role dendritic cells (DCs) not macrophages fibroblasts. Induction triggered by DNA viruses HSV-1 murid herpesvirus 68 RNA virus vesicular stomatitis Sendai was impaired...
Significance Trafficking of TLR3 from the ER to endolysosomes and its subsequent proteolytic cleavage are necessary for it sense viral dsRNA trigger antiviral response. Understanding how trafficking is regulated important deciphering mechanism TLR3-mediated innate immune inflammatory responses. In this study, we report that TRIM3 mediates K63-linked polyubiquitination at K831. Subsequently, polyubiquitinated recognized sorted by ESCRT complexes promote activation downstream signaling. This...
Mediator of IRF3 activation (MITA, also known as stimulator interferon genes, STING) senses the second messenger cyclic GMP-AMP (cGAMP) which is synthesized upon DNA virus infection and activates innate antiviral immune response. It has been demonstrated that activity MITA delicately regulated by various post-translational modifications including polyubiquitination. In this study, we identified deubiquitinating enzyme USP44 a positive regulator MITA. recruited to following removes K48-linked...
As one of the deadliest viruses, Ebola virus (EBOV) causes lethal hemorrhagic fevers in humans and nonhuman primates. The suppression innate immunity leads to robust systemic replication EBOV, leading enhanced transmission. However, mechanism EBOV-host interaction is not fully understood. Here, we identified multiple dysregulated genes early stage EBOV infection through transcriptomic analysis, which are highly clustered Jak-STAT signaling. VP35 VP30 were found inhibit type I interferon...
Upon viral infection, retinoic acid–inducible gene I–like receptors (RLRs) recognize RNA and trigger a series of signaling events, leading to the induction type I interferons (IFNs). These processes are delicately regulated prevent excessive harmful immune responses. In this study, we identified G patch domain-containing protein 3 (GPATCH3) as negative regulator RLR-mediated antiviral pathways. Overexpression GPATCH3 impaired virus- triggered downstream genes, whereas its knockdown had...
Photochromic biliproteins can be switched by light between two states, initiated Z/E photoisomerization of the linear tetrapyrrole chromophore. The cyanobacterium Anabaena sp. PCC 7120 contains three genes coding for such biliproteins, phytochromes (aphA/B) and one α subunit phycoerythrocyanin (pecA). (a) aphA was overexpressed in Escherichia coli with N-terminal His S tags, protein reconstituted an optimized protocol phycocyanobilin (PCB), to yield photochromic chromoprotein, PCB-AphA,...