James Thomas

ORCID: 0009-0005-2142-9599
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About
Contact & Profiles
Research Areas
  • Escherichia coli research studies
  • Salmonella and Campylobacter epidemiology
  • Viral gastroenteritis research and epidemiology
  • Natural Language Processing Techniques
  • Dermatological and Skeletal Disorders
  • Kidney Stones and Urolithiasis Treatments
  • Plant Pathogens and Fungal Diseases
  • Fecal contamination and water quality
  • Second Language Acquisition and Learning
  • Sympathectomy and Hyperhidrosis Treatments
  • Porphyrin Metabolism and Disorders
  • Ruminant Nutrition and Digestive Physiology
  • Agronomic Practices and Intercropping Systems
  • Microtubule and mitosis dynamics
  • Hepatitis B Virus Studies
  • Parathyroid Disorders and Treatments
  • Plant Pathogens and Resistance
  • Lexicography and Language Studies
  • Pasture and Agricultural Systems
  • Translation Studies and Practices
  • Protist diversity and phylogeny
  • Enzyme Structure and Function
  • Text Readability and Simplification
  • Moringa oleifera research and applications
  • Wastewater Treatment and Reuse

University of Iowa
1991-2024

University of Lethbridge
2004-2020

Cardiff and Vale University Health Board
2019

Olds College
2019

University of Georgia
2019

Masaryk University
2004-2017

Tuskegee University
2010

Pennsylvania State University
2006

Hôpital Cochin
1976-1991

University of Calgary
1984-1986

Synonymous mutations, which do not alter the protein sequence, have been shown to affect function [Sauna ZE, Kimchi-Sarfaty C (2011) Nat Rev Genet 12(10):683–691]. However, synonymous mutations are rarely investigated in cancer genomics field. We used whole-genome and -exome sequencing identify somatic 29 melanoma samples. Validation of one mutation BCL2L12 285 samples identified 12 cases that harbored recurrent F17F mutation. This led increased mRNA levels because differential targeting WT...

10.1073/pnas.1304227110 article EN Proceedings of the National Academy of Sciences 2013-07-30

Pasture bloat is a major deterrent to the grazing of alfalfa ( Medicago sativa L.) pastures by ruminants, despite high growth rates that are obtainable. Sainfoin Onobrychis viciifolia Scop.), condensed tannin‐containing legume, known reduce pasture in mixed stands. Experiments were conducted 2010 2012 at Lethbridge, AB, using two sainfoin/cultivar AC Blue J originally seeded as 50:50 mixes 2008 and rotationally grazed steers Bos taurus ). New sainfoin population cultivar LRC‐3519...

10.2134/agronj13.0378 article EN cc-by-nc-nd Agronomy Journal 2014-05-30

Dans cet article, nous introduisons l’idée d’utiliser des corpus – le nom que donnent les linguistes à collections très importantes de données dans la recherche en linguistique, et esquissons l’histoire, tout d’abord pour linguistique général avant pencher sur didactique. Nous attardons ensuite problèmes inhérents l’utilisation didactique faire propositions leur bonne utilisation : premièrement, ne pas peur aux apprenants ; utiliser quand dictionnaire suffit déguiser dictionnaire. Ensuite...

10.3917/rfla.201.0061 article FR Revue française de linguistique appliquée 2015-05-20

Abstract Raw river and irrigation water in the Oldman River Basin southern Alberta was tested for presence of two bacterial pathogens, Escherichia coli O157:H7 Salmonella spp., over last 2 yr (2000–2001). The number E. spp. isolated from raw peaked during summer months. While only 11/802 (1.3%) samples entire sampling season 2000 16/806 (2.0%) 2001, pathogen one or more times 10/35 (28.5%) sites 13/40 (32.5%) 2001. 44/802 (5.5%) 122/822 (14.9%) 2001; 25/35 (71.4%) 29/40 (72.5%) Certain had...

10.1080/15287390490492421 article EN Journal of Toxicology and Environmental Health 2004-09-09

Pure heat-stable inhibitor of the cAMP-dependent protein kinase (PKI) has been isolated in high yield by using a bacterial expression vector constructed to synthesize complete sequence rabbit muscle inhibitor, plus an amino-terminal initiator methionine and glycine. Bacterially expressed PKI inhibitory activity identical that from skeletal and, gel filtration electrophoresis, same physicochemical characteristics as native physiological form PKI. Fourier transformed infrared spectroscopy CD...

10.1016/s0021-9258(18)99105-2 article EN cc-by Journal of Biological Chemistry 1991-06-01

Abstract Fecal coliform (FC) and Escherichia coli (EC) concentrations in the Oldman River its tributaries, irrigation canals southern Alberta, Canada, were monitored during 1998, 1999 2000. High FC EC counts found drainages from agricultural lands all years downstream of City Lethbridge wastewater treatment plant (WTP) 1998. A significant decrease WTP was observed 1999, after an upgrade to WTP, which included a UV disinfection system. Spikes surface waters following heavy rainfall events. It...

10.2166/wqrj.2003.003 article EN Water Quality Research Journal 2003-02-01

Discovering English with Sketch Engine exploits the lexical turn in language teaching, as it enables reader to consult the of thousands native speakers who is authentic speech and writing has been sampled stored corpora. In process answering hundreds questions, the reader acquires corpus skills necessary obtain the language data from which conclusions can then be drawn. The questions concern collocations, idioms, phrasal verbs, prepositions, word order, colloquial language,...

10.14705/rpnet.2014.000246 article EN 2014-12-13

Deadly outbreaks and illnesses due to Shiga toxin-producing Escherichia coli (STEC) occur worldwide; however, the cultivation methods required for adequate monitoring traceback investigations are inefficient at best. Detection of STEC relies heavily on enrichment; yet no standard media or protocols exist. Furthermore, whether enrichment may bias detection multiple serogroups from complex samples is unknown. Thus, 14 strains O157 top six non-O157s (O26, O45, O103, O111, O121, O145) were...

10.1139/facets-2016-0007 article EN cc-by FACETS 2016-08-17

Long-term trends in alpine and prairie snow pack accumulation melt are affecting streamflow within the Oldman River Basin southern Alberta, Canada. Unchecked rural urban development also has contributed to changes water quality, including enhanced microbial populations increased water-borne pathogen occurrence. In this study we look at changing environment its impact on quality quantity. The cumulative effects include a decline net supplies, declining resulting risk of disease. Our data...

10.2166/wst.2006.328 article EN Water Science & Technology 2006-05-01

Shiga toxin-producing Escherichia coli (STEC) are a subgroup of E. causing human diseases. Methods to control STEC in livestock and humans limited. These other emerging pathogens global concern novel mitigation strategies required. Habitats populated by bacteria subjected competition pressures due limited space resources but they use various compete natural environments. Our objective was evaluate non-pathogenic strains isolated from cattle feces for their ability out-compete STEC....

10.3390/vetsci5040093 article EN cc-by Veterinary Sciences 2018-11-02

Wheat is affected by many fungal diseases that can cause severe yield and quality losses. Disease prediction models generally employ weather data to estimate potential for infection determine timing fungicide applications, but these fail account the presence quantity of pathogen inoculum. This study adapted highly specific qPCR primers identify quantify, in real-time, inoculum present air six most important wheat pathogens Canada. Fungal spores were collected using either Burkard spore...

10.1080/07060661.2020.1817795 article EN Canadian Journal of Plant Pathology 2020-11-27

Shiga toxin-producing Escherichia coli (STEC) are bacterial pathogens responsible for deadly foodborne outbreaks and sporadic illnesses globally. Children under five most susceptible to severe complications death. Seven main serogroups (O157 top six non-O157: O26, O45, O103, O111, O121, O145) have been identified as causing the majority of STEC infections in humans. Beef products one frequent source infection, necessitating robust surveillance programs. However, detection isolation methods...

10.2527/af.2016-0021 article EN cc-by-nc Animal Frontiers 2016-04-01

From January to December 1983, 12,207 specimens of urine were examined for ova Schistosoma haematobium and 753 (6.17%) positive. this group, 44 adult patients investigated urinary tract abnormalities. Haematuria was the commonest presenting symptom (81.8%) followed by lower abdominal pain (77.3%) dysuria (68.2%). Urinalysis revealed proteinuria in 55.5%, leucocyturia 90.9% microhaematuria 88.6% patients. Twenty-four hour protein excretion ranged from 230 mg 2.2 g (mean 960 mg). Serum...

10.1080/00034983.1986.11812073 article EN Annals of Tropical Medicine and Parasitology 1986-12-01

Previous research showed that the consumption of heat-killed E. coli O157:H7 bacteria resulted in an increase level DNA damage intestine, liver and spleen cells. We hypothesized certain bacterial components released from trigger this response. analysed possibility [such as lipopolysaccharides (LPS)] could induce changes proteins involved cell proliferation, repair methylation distal tissues mice. Four-week-old male mice were provided water supplemented with whole or (DNA, RNA, LPS). Spleen...

10.1002/em.21787 article EN Environmental and Molecular Mutagenesis 2013-06-28

As population growth places greater pressures on potable water supplies, nonpotable recycled irrigation is becoming widely used turfgrass areas including golf courses, sports fields, parks, and lawns. Nonpotable waters often have elevated salinity levels, therefore turfgrasses must, increasingly, good tolerance to persist in these environments. This greenhouse study evaluated 10 commonly cultivars representing warm-season species of bermudagrass ( Cynodon spp . ), zoysiagrass Zoysia st....

10.21273/hortsci13963-19 article EN HortScience 2019-09-01

Free radicals are formed as a result of cellular processes and play key role in predisposition to development numerous diseases premature aging. Recently, we reported the syntheses number novel phenolic antioxidants for possible application food industry. In present study, analyses molecular gene expression effects some normal human tissues cancer cells were undertaken. Results indicated that whereas examined showed no on morphology oral gingival epithelial tissues, they exerted profound...

10.4161/cbt.25935 article EN Cancer Biology & Therapy 2013-09-16

Bacterial infection has been linked to carcinogenesis, however, there is lack of knowledge molecular mechanisms that associate with the development cancer. We analyzed possible effects consumption heat-killed E. coli O157:H7 cells or its cellular components, DNA, RNA, protein lipopolysaccharides (LPS) on gene expression in naïve liver cells. Four week old mice were provided water supplemented whole bacteria bacterial components for a two period. One group animals was sacrificed immediately,...

10.1371/journal.pone.0067342 article EN cc-by PLoS ONE 2013-07-09

Distillers dried grains with solubles (DDGS) are a coproduct of the ethanol industry and often used as replacement for grain in livestock production. Feeding corn DDGS to cattle has been linked increased fecal shedding Escherichia coli O157:H7, although Canada, produced from wheat. This study assessed effects including 22.5% wheat or (DM basis) into barley-based diets on performance, carcass characteristics, animal health, E. O157:H7 commercial feedlot cattle. Cattle (n = 6,817) were...

10.2527/jas.2011-4856 article EN Journal of Animal Science 2012-06-04

Often Escherichia coli are harmless and/or beneficial bacteria inhabiting the gastrointestinal tract of livestock and humans. However, Shiga toxin-producing E. (STEC) have been linked to human disease. Cattle primary reservoir for STEC "super-shedders" considered be a major contributor in animal transmission. Among STEC, O157:H7 is most recognized serotype, but recent years, non-O157 increasingly In Argentina Germany, O178 an emerging pathogen. Our objective was compare populations O178,...

10.1371/journal.pone.0195880 article EN cc-by PLoS ONE 2018-04-12

A multi-year, multi-location grazing study with alfalfa (Medicago sativa L.) and sainfoin (Onobrychis viciifolia Scop.) mixed pastures was undertaken to determine pasture productivity quality. At Lethbridge, of 3 experimental populations (LRC-3401, LRC-3432, LRC-3519) Nova AC Blue J were established in a replicated experiment 2008. similar Beaver 2009 at Swift Current, Saskatchewan, Canada (SC). The developed persist mixtures under multiple-cut system. Pastures grazed by steers using...

10.15232/pas.2016-01560 article EN cc-by-nc-nd The Professional Animal Scientist 2017-07-27
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