A5104248935- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Glaucoma and retinal disorders
- Lysosomal Storage Disorders Research
- Histone Deacetylase Inhibitors Research
- Traumatic Brain Injury Research
- Traumatic Brain Injury and Neurovascular Disturbances
- RNA regulation and disease
- Autophagy in Disease and Therapy
- Photoreceptor and optogenetics research
- Traumatic Ocular and Foreign Body Injuries
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Protein Tyrosine Phosphatases
- Calpain Protease Function and Regulation
- Neurological Disorders and Treatments
- Nuclear Receptors and Signaling
- Drug-Induced Ocular Toxicity
- Bone and Dental Protein Studies
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Cancer Research and Treatments
- Ocular Oncology and Treatments
- Connexins and lens biology
- Parkinson's Disease Mechanisms and Treatments
University of Nebraska Medical Center
2024-2025
East Tennessee State University
2020-2024
University of Kentucky
2013-2023
Iowa State University
2011-2020
Visual Sciences (United States)
2016
Iowa City VA Health Care System
2012
Mutations in myocilin (MYOC) are the most common genetic cause of primary open angle glaucoma (POAG), but mechanisms underlying MYOC-associated not fully understood. Here, we report development a transgenic mouse model POAG caused by Y437H MYOC mutation; mice referred to herein as Tg-MYOCY437H mice. Analysis adult mice, which showed express human containing mutation within relevant eye tissues, revealed that they display phenotypes (i.e., elevated intraocular pressure [IOP], retinal ganglion...
Purpose.: To evaluate retina and optic nerve damage following experimental blast injury. Methods.: Healthy adult mice were exposed to an overpressure wave using a custom-built chamber. The effects of exposure on function structure evaluated the pattern electroretinogram (pERG), spectral domain optical coherence tomography (OCT), chromatic pupil light reflex. Results.: Assessment response demonstrated decreased maximum constriction diameter in blast-injured red or blue stimuli 24 hours after...
Purpose.: Mutations in the myocilin gene (MYOC) are most common known genetic cause of primary open-angle glaucoma (POAG). The purpose this study was to determine whether topical ocular sodium 4-phenylbutyrate (PBA) treatment rescues phenotypes a mouse model myocilin-associated (Tg-MYOCY437H mice). Methods.: Tg-MYOCY437H mice were treated with PBA eye drops (n = 10) or sterile PBS 8) twice daily for 5 months. Long-term safety and effectiveness (0.2%) on examined by measuring intraocular...
In 2024, there will be an estimated 1,466,718 cases of prostate cancer (PC) diagnosed globally, which 299,010 are to from the US. The typical clinical approach for PC involves routine screening, diagnosis, and standard lines treatment. However, not all patients respond therapy subsequently with treatment emergent neuroendocrine (NEPC). There currently no approved treatments this form aggressive PC. review, a compilation trials regimen treat late-stage NEPC using novel targets and/or...
Histones serve as a major carrier of epigenetic information in the form post-translational modifications which are vital for controlling gene expression, maintaining cell identity, and ensuring proper cellular function. Loss histones aging genome can drastically impact landscape leading to altered chromatin structure changes expression profiles. In this study, we investigated age-related on histone levels acetylation retinal pigment epithelium (RPE) retina mice. We observed global reduction...
Abstract Bone metastasis continues to be the greatest challenge in treating prostate cancer (PCa) patients, despite ongoing research. In bone, PCa tumors hijack normal bone remodeling processes drive progression. However, it is unclear how these interactions BM-PCa growth environment. To understand mechanisms associated with regulation of MSCs, we previously identified that induces MSC expression pro-inflammatory chemokine CXCL8 and its mouse functional homolog Cxcl1. date, there has been...
Abstract Objective To perform in vivo analysis of retinal functional and structural parameters healthy mouse eyes. Animal Studied Adult C57BL/6 male mice ( n = 37). Procedures Retinal function was evaluated using pattern electroretinography (pERG) the chromatic pupil light reflex (cPLR). Structural properties retina nerve fiber layer (NFL) were spectral‐domain optical coherence tomography (SD‐OCT). Results The average pERG amplitudes found to be 11.2 ± 0.7 μV (P50‐N95, mean SEM), with an...
Abstract Objective To perform detailed analysis of retinal changes in dogs with SARDS using optical coherence tomography ( OCT ), funduscopy, and molecular analysis. Animals Subjects were 29 from 12 US states Canada diagnosed by 8 ophthalmologists. An additional 7 eyes 5 deceased used for histological Procedures Dogs evaluated chromatic pupil light reflex testing cPLR electroretinography ERG ); subjects underwent complete ophthalmic examination, including photography, , addition to...
B-cell receptor (BCR) signaling is a central driver in chronic lymphocytic leukemia (CLL), along with the activation of pro-survival pathways (e.g., NF-κB) and aberrant anti-apoptotic mechanisms BCL2) culminating to CLL cell survival drug resistance. Front-line targeted therapies such as ibrutinib (BTK inhibitor) venetoclax (BCL2 have radically improved management. Yet, persisting cells lead relapse ~20% patients, signifying unmet need inhibitor-resistant refractory CLL. SpiD3 novel...
The CRISPR/Cas9 system is a robust, efficient, and cost-effective gene editing tool widely adopted in translational studies of ocular diseases. However, vivo CRISPR-based animal models poses challenges such as the efficient delivery CRISPR components viral vectors with limited packaging capacity Cas9-associated immune response. Using germline Cas9-expressing mouse model would help to overcome these limitations. Here, we evaluated long-term effects SpCas9 expression on retinal morphology...
Juvenile neuronal ceroid lipofuscinosis (JNCL, aka. juvenile Batten disease or CLN3 disease), a lethal pediatric neurodegenerative without cure, often presents with vision impairment and characteristic ophthalmoscopic features including focal areas of hyper-autofluorescence. In the associated research article "Loss CLN3, gene mutated in lipofuscinosis, leads to metabolic autophagy induction retinal pigment epithelium" (Zhong et al., 2020) [1], we reported observations autofluorescent lesions...
Abstract Objective To describe functional and structural features of presumed cancer‐associated retinopathy (CAR) mimicking sudden acquired retinal degeneration syndrome (SARDS) in dogs treatment outcomes. Animals Subjects were 17 from 8 eight US states Canada diagnosed with SARDS or immune‐mediated retinitis (IMR) by 12 ophthalmologists. Nine eyes seven deceased patients used for microarray (MA), histology, immunohistochemical (IHC) analysis. Procedures Dogs underwent complete ophthalmic...