A5027883075- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- Force Microscopy Techniques and Applications
- 3D Printing in Biomedical Research
- RNA Research and Splicing
- Axon Guidance and Neuronal Signaling
- Advanced Fluorescence Microscopy Techniques
- Lipid Membrane Structure and Behavior
- Silk-based biomaterials and applications
- Wnt/β-catenin signaling in development and cancer
- Viral Infectious Diseases and Gene Expression in Insects
- Near-Field Optical Microscopy
- Hippo pathway signaling and YAP/TAZ
- Microfluidic and Bio-sensing Technologies
- Biochemical and Structural Characterization
- Calpain Protease Function and Regulation
- Protein Kinase Regulation and GTPase Signaling
- Caveolin-1 and cellular processes
- Graphene and Nanomaterials Applications
- Genomics and Chromatin Dynamics
- Integrated Circuits and Semiconductor Failure Analysis
- HER2/EGFR in Cancer Research
- Developmental Biology and Gene Regulation
- S100 Proteins and Annexins
- Neurogenetic and Muscular Disorders Research
National University of Singapore
2014-2025
Agency for Science, Technology and Research
2018
Institute of Molecular and Cell Biology
2011
Tata Institute of Fundamental Research
2007
Transmembrane signaling receptors, such as integrins, organize nanoclusters that provide several advantages, including increasing avidity, sensitivity (increasing the signal-to-noise ratio), and robustness (signaling threshold) of signal in contrast to by single receptors. Furthermore, compared large micron-sized clusters, offer advantage rapid turnover for disassembly signal. However, whether function hubs remains poorly understood. Here, we employ fluorescence nanoscopy combined with...
Border cells perform a collective, invasive, and directed migration during Drosophila melanogaster oogenesis. Two receptor tyrosine kinases (RTKs), the platelet-derived growth factor/vascular endothelial factor-related (PVR) epidermal factor (EGFR), are important for reading guidance cues, but how these cues steer is not well understood. During collective migration, front, back, side extensions dynamically project from individual within group. We find that input both RTKs affects presence...
Cell growth depends upon formation of cell-matrix adhesions, but mechanisms detailing the transmission signals from adhesions to control proliferation are still lacking. Here, we find that scaffold protein talin undergoes force-induced cleavage in early produce rod fragment is needed for cell cycle progression. Expression noncleavable blocks growth, adhesion maturation, proper mechanosensing, and related property EGF activation motility. Further, expression presence full-length rescues other...
Failure of neural tube closure during embryonic development can result in anencephaly, one the most common birth defects humans. A family with recurrent anencephalic fetuses was investigated to understand its etiology and pathogenesis. Exome sequencing revealed a recessive germline 21-bp in-frame deletion NUAK2 segregating disease. In vitro kinase assays demonstrated that 7–amino acid truncation NUAK2, serine/threonine kinase, completely abrogated catalytic activity. Patient-derived disease...
Abstract Nanoscale organization of transmembrane receptors is critical for cellular functions, enabled by the nanoscale engineering bioligand presentation. Previously, a spatial threshold ≤60 nm integrin binding ligands in cell–matrix adhesion demonstrated using monoliganded gold nanoparticles. However, ligand geometric arrangement limited to hexagonal arrays monoligands, while plasmonic quenching limits further investigation fluorescence‐based high‐resolution imaging. Here, these...
Cell adhesion receptors are transmembrane proteins that bind cells to their environment. These typically cluster into disk-shaped or linear structures. Here, we show such clustering patterns spontaneously emerge when the receptor senses membrane deformation gradient, for example, by reaching a lower-energy conformation is tilted relative underlying binding substrate. Increasing strength of gradient-sensing mechanism first yields isolated clusters and then long Our theory coherent with...
Nano-Biopatterning In article number 2309284 by Haogang Cai, Rishita Changede, and co-workers, a dielectric nano-biopatterning technology is developed, enabling super-resolution fluorescence microscopy that challenging for conventional plasmonic nanopatterns with quenching effects. Through both simulations experiments, nanopattern interactions adjacent surrounding lipid membranes are studied. This will facilitate precise quantitative cell biology studies to investigate molecular-scale...
Upon interaction with the extracellular matrix, integrin receptors form nanoclusters as a first biochemical response to ligand binding. Here, we uncover critical biodesign principle where these are spatially self-organized, facilitating effective mechanotransduction. Mouse Embryonic Fibroblasts (MEFs) β3 organized themselves an intercluster distance of ~550 nm on uniformly coated fibronectin substrates, leading larger focal adhesions. We determined that this spatial organization was driven...
Abstract Transmembrane signalling receptors, such as integrins, organise nanoclusters that are thought to provide several advantages including, increasing avidity, sensitivity (increasing the signal-to-noise ratio) and robustness (signalling above a threshold rather than activation by single receptor) of signal compared receptors. Compared large micron-sized clusters, offer advantage rapid turnover for disassembly signal. However, if function hubs remains poorly understood. Here, we employ...
Widefield stochastic microscopy techniques such as PALM or STORM rely on the progressive accumulation of a large number frames, each containing scarce super-resolved point images. We justify that redundancy in localization detected events imposes specific limit temporal resolution. Based theoretical model, we derive analytical predictions for minimal time required to obtain reliable image at given spatial resolution, called completion time. In contrast standard assumptions, find scales...
Abstract Integrin-mediated cell matrix adhesions are key to sensing the geometry and rigidity of extracellular environment regulate vital cellular processes. In vivo , (ECM) is composed a fibrous mesh. To understand that supports adhesion formation on substrates, we patterned 10 nm gold-palladium single lines or pairs (total width within 100 nm), mimicking thin ECM fibers minimal mesh geometry, respectively functionalized it with integrin binding ligand Arg-Gly-Asp (RGD). Single showed...
Cell adhesion proteins are transmembrane that bind cells to their environment. These typically cluster into disk-shaped or linear structures. Here we show such clustering patterns spontaneously emerge when the protein sense membrane deformation gradient, for example by reaching a lower-energy conformation is tilted relative underlying binding substrate. Increasing strength of gradient-sensing mechanism first yields isolated clusters and then long Our theory coherent with experimental...
Abstract Chemokine signaling via growth factor receptor tyrosine kinases (RTKs) regulates development, differentiation, and disease implying that it is involved in a myriad of cellular processes. A single RTK, for example the Epidermal Growth Factor Receptor (EGFR), used repeatedly multitude developmental programs. Quantitative differences magnitude duration RTK can bring about different outcomes. Understanding this complex signals requires real time visualization signal. To visualize...