A5055535849- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Micro and Nano Robotics
- Protist diversity and phylogeny
- Laser-Matter Interactions and Applications
- Cell death mechanisms and regulation
- Plant Stress Responses and Tolerance
- Laser-induced spectroscopy and plasma
- Microtubule and mitosis dynamics
- Genetic and Kidney Cyst Diseases
- CRISPR and Genetic Engineering
- Biofuel production and bioconversion
- Plant Molecular Biology Research
- Biocrusts and Microbial Ecology
- Bacterial Genetics and Biotechnology
- Diabetes Treatment and Management
- RNA Research and Splicing
- Pancreatic function and diabetes
- Curcumin's Biomedical Applications
- Plant Reproductive Biology
- Nanoparticles: synthesis and applications
- Plant Gene Expression Analysis
- Orbital Angular Momentum in Optics
- RNA modifications and cancer
- Metabolism, Diabetes, and Cancer
Center for Excellence in Basic Sciences
2015-2024
University of Mumbai
2013-2024
Manipal Academy of Higher Education
2017-2020
Center for Excellence in Education
2014
Department of Atomic Energy
2013
Tata Institute of Fundamental Research
1990-2009
Chlamydomonas reinhardtii (Ehrenberg) cells exhibited cell death process akin to that of apoptosis when exposed ultraviolet (UV)‐C irradiation (1–100 J/m 2 ). We observed typical hallmarks including shrinkage, associated nuclear morphological changes, flipping phosphatidylserine, and DNA fragmentation detected by the terminal deoxynucleotidyl transferase‐mediated dUTP nick end‐labeling assay oligonucleosomal laddering assay. Interestingly, fluorescence imaging changes in UV‐C cells,...
Eukaryotic microalgae serve as indicators of environmental change when exposed to severe seasonal fluctuations. Several stress conditions are known produce reactive oxygen species in cellular compartments, resulting oxidative damage and apoptosis. The study cell death higher plants animals has revealed the existence an active 'programmed death' (PCD) process similarities between such processes suggest evolutionary origin. A was undertaken examine morphological, biochemical molecular...
We probe femtosecond laser induced damage to aqueous DNA, relying on strong-field interaction with water wherein electrons and free radicals are generated in situ; these, turn, interact DNA plasmids under physiological conditions, producing nicks. Exposure intense pulses of 1350 2200 nm light induces single strand breaks double (DSBs) DNA. At the longer wavelength (and at higher intensities), rotationally hot OH induce DSBs, linear Strand occur due or multiple hits With light, DSBs formed...
Single strand breaks are induced in DNA plasmids, pBR322 and pUC19, aqueous media exposed to strong fields generated using ultrashort laser pulses (820 nm wavelength, 45 fs pulse duration, 1 kHz repetition rate) at intensities of 1-12 TW cm(-2). The generate, situ, electrons radicals that induce transformation supercoiled into relaxed DNA, the extent which is quantified. Introduction electron radical scavengers inhibits damage; results indicate OH primary (but not sole) cause damage.
Menadione, a quinone that undergoes redox cycles leading to the formation of superoxide radicals, induces programmed cell death (PCD) in animals and plants. In this study, we investigated whether unicellular green alga Chlamydomonas reinhardtii P.A.Dangeard is capable executing PCD upon exposure menadione stress. We report here, morphological, molecular, biochemical changes after C. cells. The effect on has been shown be dose-dependent; 5-100 μM causes 20%-46% death, respectively. It appears...
Flagella and cilia are fine thread-like organelles protruding from cells that harbour them. The typical ‘9 + 2’ confer motility on these cells. Although the mechanistic details of remain elusive, dynein-driven is regulated by various kinases phosphatases. A-kinase anchoring proteins (AKAPs) scaffolds bind to a variety such proteins. Usually, they known possess dedicated domain in vitro interacts with regulatory subunits (RI RII) present cAMP-dependent protein kinase (PKA) holoenzyme. These...
:Programmed cell death (PCD) plays an important role in mediating adaptation responses under adverse conditions such as high salinity. To understand the molecular mechanism of algal cells to salt, freshwater alga Chlamydomonas reinhardtii was challenged with 200 mM KCl. In present study, vegetative C. undergo when exposed KCl, this being dose-dependent, 100–800 KCl causing 16–64% death. Within half hour exposure, a ~1.9-fold rise intracellular H2O2 content followed increase antioxidant...
We report on damage to DNA in an aqueous medium induced by ultrashort pulses of intense laser light 800 nm wavelength. Focusing such pulses, using lenses various focal lengths, induces plasma formation within the medium. Such can have a spatial extent that is far excess Rayleigh range. In case water, resulting ionization and dissociation gives rise situ generation low-energy electrons OH-radicals. Interactions these with plasmid produce nicks backbone: single strand breaks (SSBs) are as are,...
We report here, the transcriptional regulation of 2 Calcium Dependent Protein Kinases in response to nutrient starvation Chlamydomonas reinhardtii vegetative cells. The CDPK proteins, CDPK1 and CDPK3; share 53% identity among themselves, a maximum 57% 52% higher plants respectively 42% apicomplexan protozoans. expressed CDPK1-GFP fusion protein C. cells showed its distribution both cell body membrane-matrix fraction flagella. exhibits mobility shift presence Ca2+, confirming Ca2+-binding...
There is a need for continued development of acetylcholinesterase (AChE) inhibitors that could prolong the life acetylcholine in synaptic cleft and also prevent aggregation amyloid peptides associated with Alzheimer's disease. The lack 3D-QSAR model which specifically deconvulates type interactions quantifies them terms energies has motivated us to report CoRIA vis-à-vis standard methods, CoMFA CoMSIA. was found be statistically superior CoMSIA models it efficiently extract key residues...
Expression of affinity-tagged recombinant proteins for crystallography, protein-protein interaction, antibody generation, therapeutic applications, etc. mandates the generation high-yield soluble proteins. Although recent developments suggest use yeast, insect, and mammalian cell lines as protein expression platforms, Escherichia coli is still most popular, due mainly to its ease growth, feasibility in genetic manipulation economy. However, some have a spontaneous tendency form inclusion...
Escherichia coli BolA protein is a stress-inducible morphogene, regulates transcription, forms biofilms and interacts with monothiol glutaredoxins. Its presence has been documented in plants but its role remains enigmatic. This study attempts to functionally dissect the of BolA-domain-containing alga Chlamydomonas reinhardtii. Of five C. reinhardtii bolA-like genes annotated for BolA-domain, open reading frame highest similarity algal systems was cloned over-expressed E. coli....
Apoptosis is a naturally occurring process during the growth and development of multicellular organisms increasingly active times cellular stress such as in response to intracellular DNA damage when removal host cell paramount prevent cancer. Unfortunately, once formed, cancer cells become impervious apoptosis, creating desperate need identify an approach induce apoptosis these cells. An attractive option focus efforts on developing locating compounds which activate using natural compounds....