A5015405409- Erythrocyte Function and Pathophysiology
- Enzyme function and inhibition
- Pancreatic function and diabetes
- Adenosine and Purinergic Signaling
- Biochemical and Molecular Research
- Hemoglobin structure and function
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Neonatal Health and Biochemistry
- Metabolomics and Mass Spectrometry Studies
- Protein Structure and Dynamics
- Diet, Metabolism, and Disease
- Genetic Associations and Epidemiology
- Mass Spectrometry Techniques and Applications
- Peroxisome Proliferator-Activated Receptors
- RNA Interference and Gene Delivery
- Peptidase Inhibition and Analysis
- Hemoglobinopathies and Related Disorders
- Advanced Chemical Sensor Technologies
- Alcohol Consumption and Health Effects
- Chemical Reaction Mechanisms
- Aldose Reductase and Taurine
- Clinical Laboratory Practices and Quality Control
- Sexual Differentiation and Disorders
- Virus-based gene therapy research
- Renal function and acid-base balance
University of Southern California
2023-2025
King's College London
1967-1985
King's College - North Carolina
1985
Universidad de Londres
1967-1980
University of London
1961-1980
University College London
1968-1980
King's College Hospital
1977-1979
University of Cambridge
1972
Medical Research Council
1963-1968
Summary A new and specific method for the study of adenosine deaminase isozymes is described. Examination red‐cell lysates has revealed three genetically determined electrophoretically different ADA phenotypes: 1, 2–1 2. Family studies indicate that these phenotypes are by two alleles, 1 2 at an autosomal locus. Preliminary population data suggest a frequency about 0.06 in European, 0.04 Negroes 0.11 Asiatic Indians. The behaviour isozyme pattern on storage or after treatment with thiol...
BACKGROUND: COVID-19 is associated with acute risk of major adverse cardiac events (MACE), including myocardial infarction, stroke, and mortality (all-cause). However, the duration underlying determinants heightened cardiovascular disease MACE post–COVID-19 are not known. METHODS: Data from UK Biobank was used to identify cases (n=10 005) who were positive for polymerase chain reaction (PCR + )-based tests SARS-CoV-2 infection (n=8062) or received hospital-based International Classification...
1. The partial purification of adenosine deaminase, types 1, 2 and 2-1, from human erythrocytes is described. 2. isoenzyme components characteristic the three forms enzyme were partially resolved by chromatography on DEAE-Sephadex. 3. Gel various gave estimates molecular weights in range 30000-35000. 4. Electrophoresis starch gels containing increasing percentages did not reveal any differences weight between genetic variants or their components. 5. Analytical isoelectric-focusing...
1. The haemoglobin content of developing erythroblasts was shown to increase rapidly when the cells completed final cell division erythroid development and passed from dividing into non-dividing compartment. 2. activity carbonic anhydrase measured continually throughout differentiation. increased most in polychromatic stage. 3. Catalase did not significantly during differentiation until reticulocyte 4. four enzymes, glucose 6-phosphate dehydrogenase, 6-phosphogluconate adenosine deaminase...
Background: Circulating glycine levels have been associated with reduced risk of coronary artery disease (CAD) in humans but these associations not observed all studies. We evaluated whether the relationship between and atherosclerosis was causal using genetic analyses feeding studies mice. Methods: Serum were for association CAD UK Biobank. Genetic determinants identified through a genome-wide study (GWAS) used to evaluate by Mendelian randomization (MR). A dietary supplementation carried...
SUMMARY Red cell glucose‐6‐phosphate dehydrogenase (G‐6‐PD) activity was measured in fourteen XXX , eleven XX Y three XO individuals, four individuals who were 17 but had testicular feminization, and appropriate male female controls. No evidence for any quantitative relationship between the number of X‐chromosomes present level red‐cell G‐6‐PD observed. Since is known to be determined by at least one gene locus X‐chromosome, these results agreement with others imply complete dosage...
1. The partial purification of purine nucleoside phosphorylase from rabbit erythrocytes is described. 2. Analytical and preparative isoelectric focusing gave a pI value for the enzyme 4.65. 3. Gel-chromatography sucrose-density-gradient-centrifugation techniques estimates molecular weight in range 75000-83000. 4. Lineweaver-Burk plots kinetic data were non-linear at high inosine concentrations. Extrapolation linear part such yielded Km about 70 micrometer erythrocyte liver enzymes. 5. A Hill...
1. The partial purification of adenylate kinase, types 1 and 2, from human erythrocytes is described. 2. Gel chromatography both forms the enzyme gave estimates molecular weights in range 20000-23000. 3. Studies on crude haemolysates at various pH values indicated that type 2 was less stable than Heat denaturation studies partially purified enzymes confirmed these findings. 4. Measurements rates inhibition by iodoacetate iodoacetamide showed reacts more readily with reagents. 5. effect...
Summary 1. Staining procedures are described for the detection after starch‐gel electrophoresis of ribose‐5‐phosphate isomerase (RPI) and ribulose 5‐phosphate 3‐epimerase (RPE). 2. Both RPI RPE were detected in all human tissues including red cells, lymphocytes fibroblasts. 3. No evidence was found more than one structural gene locus either enzyme. 4. NO allelic variants enzyme erythrocyte lysates from over 200 unrelated individuals. 5. Preliminary data presented which suggest that...
A direct assay procedure is described for D-ribulose 5-phosphate 3-epimerase (EC 5.1.3.1) which exploits differences in the c.d. spectra of substrate and product. The enzyme has been purified from human erythrocytes was resolved by gel filtration sucrose-density-gradient centrifugation into a major component apparent Mr 45 000 minor 23 000. Electrophoresis sodium dodecyl sulphate gave single corresponding to Kinetic data indicate dissociation dimeric form monomers low specific activity;...
1. Erythrocyte carbonic anhydrase I was measured by immunoelectrophoresis in blood samples taken at intervals during the menstrual cycle of 26 normal women. Plasma progesterone concentrations were also 18 these subjects. In every case, erythrocyte concentration reached a maximum that point when there secretion progesterone. Statistical analysis indicated observed changes are highly significant and plasma significantly correlated. 2. three pregnant subjects showed steady increase up to term....
1. The kinetic properties of two genetic variants human erythrocyte adenylate kinase were studied at limiting concentrations both ADP and MgADP(-) in the forward direction AMP MgATP(2-) reverse direction. 2. Primary reciprocal plots rule out possibility a Ping Pong mechanism for forms enzyme. 3. Analysis data by an appropriate computer program gave following K(m) values type 1 enzyme: AMP, 0.33mm+/-0.1; MgATP(2-), 0.95mm+/-0.13; ADP, 0.12mm+/-0.03; MgADP(-), 0.22mm+/-0.04. Values 2 enzyme...
1. Concave-downward double-reciprocal plots were obtained for rabbit erythrocyte purine nucleoside phosphorylase when the concentration of Pi was varied over a wide range at fixed saturating either inosine or deoxyinosine. Similar behaviour also displayed by calf spleen enzyme. 2. The degree curvature greatly modified presence SO42-, introduced into assay mixture with linking enzyme xanthine oxidase; competitive inhibition SO42- observed narrow high concentrations. 3. Partial inactivation...