A5115704076- Electrochemical sensors and biosensors
- Molecular Sensors and Ion Detection
- Diatoms and Algae Research
- Protist diversity and phylogeny
- Marine and coastal ecosystems
- Click Chemistry and Applications
- Photosynthetic Processes and Mechanisms
- Porphyrin and Phthalocyanine Chemistry
- Algal biology and biofuel production
- Analytical Chemistry and Sensors
- Advanced Fluorescence Microscopy Techniques
- Biotin and Related Studies
Lomonosov Moscow State University
2024-2025
Fluorescence-lifetime imaging microscopy (FLIM) is a powerful technique for highly multiplexed in live cells. In this work, we present genetically encoded FLIM multiplexing platform based on combination of fluorogen-activating protein FAST and red-shifted fluorogen N871b from the arylidene-imidazolone family. We showed that series mutants exhibit similar steady-state optical properties complex with but have different fluorescence lifetimes. The brightness binding strength pairs these...
Molecular mechanisms underlying the green insect camouflage have puzzled researchers for over a century. Here, we isolated and identified water-soluble protein from integument of bush-cricket Tettigonia cantans . De novo sequencing cloning revealed severely fragmented form vitellogenins, ubiquitous multifunctional, but still largely enigmatic glycolipoproteins essential embryonic development lacking structural characterization. The distinctive color chromoprotein results binding remarkable...
Photonic-based methods are crucial in biology and medicine due to their non-invasive nature, allowing remote measurements without affecting biological specimens. The study of diatoms using advanced photonic remains a relatively underexplored area, presenting significant opportunities for pioneering discoveries. This research provides comprehensive analysis marine diatoms, specifically Nitzschia sp., across varying salinity levels, integrating fluorescence lifetime imaging microscopy (FLIM),...