A5082240781- Escherichia coli research studies
- Probiotics and Fermented Foods
- Gut microbiota and health
- Animal Nutrition and Physiology
- Archaeology and Natural History
- Viral gastroenteritis research and epidemiology
- Vibrio bacteria research studies
- Salmonella and Campylobacter epidemiology
- Birth, Development, and Health
- Legionella and Acanthamoeba research
- Toxoplasma gondii Research Studies
- Microbial Metabolites in Food Biotechnology
- Water Quality and Resources Studies
- Immune Response and Inflammation
- Atmospheric and Environmental Gas Dynamics
- Photoacoustic and Ultrasonic Imaging
- Hydrocarbon exploration and reservoir analysis
- Veterinary medicine and infectious diseases
- T-cell and B-cell Immunology
- bioluminescence and chemiluminescence research
- Bacterial Identification and Susceptibility Testing
- Drug Transport and Resistance Mechanisms
- Hepatitis Viruses Studies and Epidemiology
- Pregnancy and preeclampsia studies
- Enterobacteriaceae and Cronobacter Research
University of South Florida
1986-2020
University of North Carolina Health Care
2018
University of North Carolina at Chapel Hill
2018
École Polytechnique Fédérale de Lausanne
2018
Imperial College London
2010-2017
Medical Research Council
2014-2016
Animal and Plant Health Agency
2012-2014
MRC Laboratory for Molecular Cell Biology
2013
University College Cork
2012
Veterinary Medicines Directorate
2009-2010
ABSTRACT Legionella pneumophila is a facultative intracellular human pathogen and the etiological agent of severe pneumonia known as Legionnaires' disease. Its virulence depends on protein secretion systems, in particular, Dot/Icm type IV system (T4SS), which essential to establish replication-permissive vacuole macrophages. The analysis role these systems their substrates for pathogenesis requires easy-to-use models approximate infection. We examined effectiveness larvae wax moth Galleria...
Legionella pneumophila, the causative agent of a severe pneumonia named Legionnaires' disease, is an important human pathogen that infects and replicates within alveolar macrophages. Its virulence depends on Dot/Icm type IV secretion system (T4SS), which essential to establish replication permissive vacuole known as containing (LCV). L. pneumophila infection can be modeled in mice however most mouse strains are not permissive, leading search for novel models. We have recently shown larvae...
The authors analyzed Illinois vital records to determine the intergenerational birth weight patterns among descendants of US-born and foreign-born White African-American women. Among generation 1 women (n = 91,061), 3 females had a 65 g more than that their 2 mothers (p < 0.0001); infants 10% lower moderately low (1,500–2,499 g) rate did mothers: 5.0% versus 5.5% percent, respectively (relative risk 0.9, 95% confidence interval: 0.9). European-born 3,339), 45 0.0001). 31,699), 17 0.001)....
ABSTRACT The potential of a prebiotic oligosaccharide lactulose, probiotic strain Lactobacillus plantarum , or their synbiotic combination to control postweaning colibacillosis in pigs was evaluated using an enterotoxigenic Escherichia coli (ETEC) K88 oral challenge. Seventy-two weanlings were fed four diets: diet (CTR), that supplemented with L. (2 × 10 CFU · day −1 ) (LPN), g kg lactulose (LAC), the two treatments (SYN). After 7 days, orally challenged. Six per treatment euthanized on days...
The prebiotic Bimuno(®) is a mixture containing galactooligosaccharides (GOSs), produced by the galactosyltransferase activity of Bifidobacterium bifidum NCIMB 41171 using lactose as substrate. Previous in vivo and vitro studies demonstrating efficacy reducing Salmonella enterica serovar Typhimurium (S. Typhimurium) colonization did not ascertain whether or protective effects could be attributed to component GOS. Here we wished test hypothesis that GOS, derived from Bimuno(®), may confer...
We investigated the role of commensals at peak infection with colonic mouse pathogen Citrobacter rodentium. Bioluminescent and kanamycin (Kan)-resistant C. rodentium persisted avirulently in cecal lumen mice continuously treated Kan. A single Kan treatment was sufficient to displace from mucosa, a phenomenon not observed following vancomycin (Van) or metronidazole (Met). Kan, Van, Met induce distinct dysbiosis, suggesting relies on specific for colonization. Expression master virulence...
Enteropathogenic Escherichia coli (EPEC), enterohaemorrhagic E. (EHEC) and Citrobacter rodentium colonize their respective hosts while forming attaching effacing lesions. Their infection strategy relies on translocation of a battery type III secretion system effectors, including Map, EspM EspT, which belong to the WxxxE/SopE family guanine nucleotide exchange factors. Using C. mouse model we found that EspT triggers expression KC TNFα in vivo. Indeed, growing body evidence suggests that,...
Enteropathogenic and enterohaemorrhagic Escherichia coli (EPEC EHEC) are diarrheagenic pathogens that colonize the gut mucosa via attaching-and-effacing lesion formation. EPEC EHEC utilize a type III secretion system (T3SS) to translocate effector proteins subvert host cell signalling sustain colonization multiplication. EspH, T3SS modulates actin dynamics, was implicated in elongation of pedestals. In this study we found EspH is necessary for both efficient pedestal formation during...
We evaluated the protective effects of fermented dairy products (FDPs) in an infection model, using mouse pathogen Citrobacter rodentium (CR). Treatment mice with FDP formulas A, B, and C or a control product did not affect CR colonization, organ specificity, attaching effacing lesion formation. Fermented A (FDP-A), but neither supernatant from FDP-A nor β-irradiated (IR) FDP-A, caused significant reduction colonic crypt hyperplasia CR-associated pathology. Profiling gut microbiota revealed...
ABSTRACT Key features of Escherichia coli K1-mediated neonatal sepsis and meningitis, such as a strong age dependency development along the gut-mesentery-blood-brain course infection, can be replicated in newborn rat. We examined temporal spatial aspects E. K1 infection following initiation gastrointestinal colonization 2-day-old (P2) rats after oral administration strain A192PP virulent bioluminescent derivative, A192PP- lux 2. A combination bacterial enumeration major organs,...
The marketplace for probiotic foods is burgeoning, measured in billions of euro per annum. It imperative, however, that all bacterial strains are fully assessed human safety. ability to bind fibrinogen considered a potential pathogenicity trait can lead platelet aggregation, serious medical complications, and some instances, death. Here we examined from species frequently used as probiotics their fibrinogen. Only one strain (CCUG 47825), Lactobacillus salivarius isolate case septicaemia, was...
The authors used 1982–1983 Illinois vital records and 1980 US Census income data to determine the contribution of maternal race risk preterm (<260 days), non-low birth weight (>2,500 g) infants. This older cohort was chosen avoid confounding effect cocaine associated with its increased local availability after 1985. In Chicago, unadjusted preterm, rate 14% for African Americans (n = 43,059) compared 9% Mexican 10,397) 7% whites 26,152) (odds ratio 1.7, 95% confidence interval 1.6-1.8; odds...
The hallmarks of enteropathogenic Escherichia coli (EPEC) infection are formation attaching and effacing (A/E) lesions on mucosal surfaces actin-rich pedestals cultured cells, both which dependent the type III secretion system effector Tir. Following translocation into cells clustering by intimin, Tir Y474 is phosphorylated, leading to recruitment Nck, activation N-WASP, actin polymerization via Arp2/3 complex. A secondary, weak, pathway triggered an NPY motif (Y454). Importantly, Y454 play...
Citrobacter rodentium, which colonizes the gut mucosa via formation of attaching and effacing (A/E) lesions, causes transmissible colonic hyperplasia. The aim this study was to evaluate whether prophylactic treatment with Bifidobacterium breve UCC2003 can improve outcome C. rodentium infection. Six-week-old albino C57BL/6 mice were pre-treated for 3 days B. breve, challenged bioluminescent administered or PBS-C 8 post-infection; control either mock-infected PBS, mock-treated PBS....
This protocol outlines the steps required to longitudinally monitor a bioluminescent bacterial infection using composite 3D diffuse light imaging tomography with integrated μCT (DLIT-μCT) and subsequent use of this data generate four dimensional (4D) movie cycle. To develop 4D movies validate DLIT-μCT for studies an IVIS Spectrum CT, we used C. rodentium, which causes self-limiting colitis in mice. In protocol, outline mice rodentium non-invasive monitoring colonization by daily enumeration...
The development of novel intervention strategies for the control zoonoses caused by bacteria such as Salmonella spp. in livestock requires appropriate experimental models to assess their suitability. Here, a porcine intestinal vitro organ culture (IVOC) model utilizing cell crown (CC) technology (CCIVOC) (Scaffdex) was developed. CCIVOC employed investigate characteristics association S. enterica serovar Typhimurium strain SL1344 with tissue following exposure Lactobacillus plantarum strain....
Tracking disease progression in vivo is essential for the development of treatments against bacterial infection. Optical imaging has become a central tool tracking population and therapeutic response. For precise understanding results terms mechanisms derived from detailed postmortem observations, however, link between two needed. Here, we develop model that provides investigation Citrobacter rodentium infection, mouse enteropathogenic Escherichia coli (EPEC). We connect C57BL/6 mice...