Surface plasmon resonance (SPR) has become an important optical biosensing technology due to its real-time, label-free, and noninvasive nature. These techniques allow for rapid ultra-sensitive detection of biological analytes, with applications in medical diagnostics, environmental monitoring, agriculture. SPR is widely used the biomolecular interactions, improvements are required both sensitivity vivo uses practical applications.

The aim of this study was to investigate the mechanism cell death by photodynamic therapy (PDT) in Rhabdomyosarcoma (RD) line. present evaluates effects with 5-ALA as photosensitizer using human muscle cancer cells experimental model. We uptake, cytotoxicity, phototoxicity, and cellular viability RD which estimated means neutral-red spectrophotometric assay. given experiment consisted two steps. For first one, were exposed at concentrations 0 up 1000 μg ALA/ml minimum essential medium (MEM)....

5-aminolevulanic acid (ALA), belonging among the promising second generation of sensitizers, was evaluated as an inducer photodamage on HeLa (human cervical adenocarcinoma) cell line. A diode laser (635 nm) used a source for initiation photodynamic effect. We studied influence different incubation times, various concentrations sensitizer, irradiation doses and combinations sensitizer light cells. Viability cells determined by means neutral red assay. The quantitative cellular uptake ALA done...

In this study we investigated the light distribution under femtosecond laser illumination and its correlation with collected diffuse scattering at surface of ex-vivo rat skin liver. The reduced coefficients μ's for liver due to different scatterers have been determined Mie-scattering theory each wavelength (800, 630, 490 nm). Absorption μa were by diffusion approximation equation in measured diffused reflectance experimentally total attenuation coefficient type tissue linearly fitting log...

The insertion of β-amino acids and replacement the amide bond with a urea in antimicrobial peptide sequences are promising approaches to enhance antibacterial activity improve proteolytic stability. Herein, we describe synthesis, characterization, short αβ cationic hybrid peptides LAU-Orn-β3,3Ac6c-PEA, DY-01; LAU-Lys-β3,3Ac6c-PEA, DY-02; LAU-Arg-β3,3Ac6c-PEA, DY-03 which C12 lipid chain is conjugated at N terminus through bonds. Further, evaluated all against both Pseudomonas aeruginosa...

Metal chalcogenide copper sulfide nanoparticles exhibit a broad spectrum of applications, encompassing solar cells, photovoltaics, optical devices, ionic materials and more. In this investigation, CuS were synthesized through facile co-precipitation method. The synthesis involved employing sulfate thiourea as precursors for Cu S, respectively. Quantitative analysis, confirming the presence Cu–S S–S bonds, was conducted Raman spectroscopy. X-ray diffraction (XRD) employed to ascertain...

5-aminolevulanic acid (ALA), a precursor of Protoporphyrin IX, was evaluated as an inducer photodamage on Hep2c, human larynx squamous cell carcinoma, line. Porphyrins are used active cytotoxic antitumor agents in photodynamic therapy (PDT). The present study evaluates the effects (PDT) with 5-aminolevulinic (5-ALA) using cells experimental model. Hep2c line irradiated red light (a diode laser, λ = 635 nm). influence different incubation times and concentrations 5-ALA, irradiation doses...

This study demonstrates the use of Raman spectroscopy for direct measurement diabetic sugar in human blood using 532 nm laser system. spectra were collected from whole drawn 21 individuals. We have elicited a reliable glucose signature patients, and measured levels serum normal, healthy patients with other malignancies like cancer hepatitis. Quantitative predictions illustrate based on molecular information carried by light highly light-scattering absorbing media. spectrum peaks are observed...

In this work Raman spectra of normal and dengue infected serum whole blood were analyzed. characteristic peaks observed when excited at 442 532 nm. all found to be blue shifted with reduced intensity. Dengue shows two 1614 1750 cm−1 which are due presence Immunoglobulin antibodies IgG IgM. Whole study provides a route information for diagnosis viral infection.

Significant apoptotic effect in Hep2c cell line has been investigated, when diode laser (λ = 635 nm, red) are used as a source of illumination and initiation photodynamic action. The optimal uptake time Photofrin® for was investigated by means spectrophotometric measurement. Quantification the live population determined neutral red assay (NRA). spectrometry measurements showed that after 46 h incubation, maximal cellular achieved photocytotoxic light dose 120 J/cm2 give effective PDT outcome...

Extensive research in biomedical optics essentially requires the determination of optical properties various biological tissues. Quantitative characterization tissues terms is achieved with an integrating sphere. However, samples having significantly higher scattering and absorption coefficients such as malignant potentially reduce signal-to-noise ratio (SNR) accuracy We describe design, implementation a modified sample holder (path length up to 1 mm) for integration Experiments conducted...

Drug delivery into the malignant cell is a basic requirement for effectiveness of photosensitizing systems photodynamic therapy (PDT). For anticancer tumoricidal drugs, e.g., 5-aminolevulinic acid (ALA), zinc oxide (ZnO) nanoparticles (NPs) are used as efficient intracellular photosensitizer carriers. Apoptotic effect drugs (ALA and Photofrin cells in presence absence ZnO NPs using confocal microscopy well Neutral Red Assay (NRA). In dark, conjugated with ALA or Photofrinhas been found to...

We presented the rapid diagnostic test for dengue infection based on light spectrum of human blood. The transmission spectra infected whole blood samples have been recorded in ultra violet to near infrared range (400 – 800 nm) about 30 conformed patients and compared normal samples. Transmission illustrate a strong band from 400 600 nm with prominant peaks at 540 580 nm, where is case below total absorption has observed. These prominent are characteristics cells damage dangue virus...

In this prospective study duly approved from Institutional Ethics Review Committee for research in medicine, PAEC General Hospital Islamabad, Pakistan, we investigate the efficacy, safety and tolerability along with cosmetic outcome of topical 5-aminolaevulinic acid photodynamic therapy superficial nonmelanoma skin cancers (NMSCs) their precursors. Patients Histological diagnosis NMSCs precursors were assessed PDT, after photographic documentation lesions written consent, underwent two (2)...

Abstract PHOTOFRIN ® was labelled with 99m Tc using SnCl 2 ·2H O as reducing agent. Instant thin layer chromatography (ITLC-SG) in 0.05 M NaOH used for evaluation of radiochemical purity. Labelling efficiency dependent on various factors that include the ligand/reductant ratio, pH and time incubation. Therefore, optimum conditions labelling were also determined. The stability Tc-PHOTOFRIN serum checked by fresh human serum. Tissue distribution evaluated Sprague Dawley rats. an >95% under...

The purpose of this study was to investigate the optical properties, absorption coefficient (μ a ) scattering s and refractive indices, (n) HeLa cell line in suspension 2% minimum essential medium (MEM) at two different (632.8 532.0 nm) wave lengths laser light. Optical properties were determined with Kubelka Munk Model (KMM) index measurement made through angle deviation method (MAD). We reported μ = 8.643 ± 0.187 2.348 0.249 cm−1 5.609 0.287 88.166 2.833 632.8 nm, respectively. Refractive...

A diversity of strategies is utilized for the diagnosis and treatment cancer with limitations side effects. Light-activated phototherapy a suitable photosensitizer can provide cure at high specificity tumor selectivity. The recent exposure nanotechnology nanoparticles in field medical science could assist patients light control presence chemical. cell death marker are directly related to nanoparticles' physiochemical composition, size, shape, self-fluorescence activation upon excitation.