Majd Al Suqri
A5094285657- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- CRISPR and Genetic Engineering
- T-cell and B-cell Immunology
Harvard University
2024
Brigham and Women's Hospital
2024
Abstract Synovial tissue inflammation is a hallmark of rheumatoid arthritis (RA). Recent work has identified prominent pathogenic cell states in inflamed RA synovial tissue, such as T peripheral helper cells; however, the epigenetic regulation these yet to be defined. Here, we examine genome-wide open chromatin at single-cell resolution 30 samples, including 12 samples with transcriptional data multimodal experiments. We identify 24 classes and predict their associated transcription factors,...
Abstract / Summary Paragraph Genetic studies have identified thousands of individual disease-associated non-coding alleles, but identification the causal alleles and their functions remain critical bottlenecks. Even though CRISPR-Cas editing has enabled targeted modification DNA, inefficient leads to heterogeneous outcomes across cells, limiting ability detect functional consequences disease alleles. To overcome these challenges, we present a multi-omic single cell sequencing approach that...