A5088733332- HIV Research and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- Hepatitis B Virus Studies
- vaccines and immunoinformatics approaches
- Plant Disease Resistance and Genetics
- Chromosomal and Genetic Variations
- CAR-T cell therapy research
- Peptidase Inhibition and Analysis
- RNA modifications and cancer
- Ovarian cancer diagnosis and treatment
- Mast cells and histamine
- Animal Genetics and Reproduction
- Galectins and Cancer Biology
- Fungal and yeast genetics research
- Herpesvirus Infections and Treatments
- Food Allergy and Anaphylaxis Research
- Cultural Competency in Health Care
- Immune responses and vaccinations
- Impact of Technology on Adolescents
- CRISPR and Genetic Engineering
- Advanced Biosensing Techniques and Applications
- HIV/AIDS Research and Interventions
Carnegie Mellon University
2024
George Mason University
2024
Microsoft (United States)
2024
University of Washington
2022-2024
Apollo Instruments (United States)
1994-1997
Leiden University
1997
University of Pennsylvania
1995-1997
Institut Pasteur de Lille
1996
King's College - North Carolina
1996
King's College London
1996
A DNA-based vaccine containing human immunodeficiency virus type 1 (HIV-1) env and rev genes was tested for safety host immune response in 15 asymptomatic HIV-infected patients who were not using antiviral drugs had CD4+ lymphocyte counts of ⩾500 per microliter blood. Successive groups received three doses (30, 100, or 300 µg) at 10-week intervals a dose-escalation trial. Vaccine administration induced no local systemic reactions, laboratory abnormalities detected. Specifically, patient...
DNA, or genetic, inoculation mimics aspects of attenuated vaccines in that synthesis specific foreign proteins is accomplished the host. These can be processed and presented on relevant major histocompatibility complex (MHC) antigens ultimately become subject immune surveillance. Very recently, we have described use new technology to generate responses mice against human immunodeficiency virus type 1 (HIV-1 ) envelope using a gp160 DNA construct. Further analysis this specifically regard HIV...
HCI research to date has only scratched the surface of unique approaches racially minoritized communities take building, designing, and using technology systems. While there been an increase in understanding how people across racial groups create community different platforms, is still a lack studies that explicitly center on Black technologists design with for their own communities. In this paper, we present findings from series semi-structured interviews who have used, created, or curated...
Abstract: An effective immune response involves the specific recognition of and elimination an infectious organism at multiple levels. In this context DNA immunization can present functional antigenic proteins to host for by all arms system, yet provides opportunity delete any genes which code antigens or pieces that may have deleterious effects. Our group has developed use nucleic acid as a possible method vaccination against Human immunodeficiency virus type 1 (HIV‐1) [1,2,3,10,11,12]....
DNA inoculation has the potential to produce antigens in a native as well host-"customized" form for presentation immune system. As such this technology may have relevance vaccine/immune therapeutic strategies variety of infectious pathogens. In rodents vivo plasmid expression vectors encoding HIV-1 gene products leads production NIV-1 vivo, resulting both cellular and humoral responses. primates only preliminary studies serology been reported. Here we report further evaluation new method...
Allergic reactions are mediated by IgE antibodies bound to high-affinity receptors on mast cells in peripheral tissues and characterized their immediacy hypersensitivity. These properties could also be advantageous immunotherapy against cancer growth tissues. We have constructed chimeric IgG1 with murine V regions human C corresponding the MOv18 monoclonal antibody ovarian tumor-associated antigen, folate binding protein. The exhibited expected affinities for antigen Fc receptors, effector...
Conventionally designed ribozymes may be unable to cleave RNA at sites which are inaccessible due secondary structure. In addition, it also difficult specifically target a conventionally ribozyme some chimeric molecules. Novel approaches for targeting were developed by using the L6 bcr-abl fusion as model. Using one approach, we successfully directed nucleation site on that is distant from GUA cleavage site. These bound substrate via an anchor sequence was complementary bcr sequences. The...
The role of the immune response in controlling human immunodeficiency virus type 1 (HIV-1) replication is controversial. Immunotherapeutic strategies that have ability to broaden responses might play a slowing disease progression. DNA immunization was studied as immunotherapy infected chimpanzees. Two HIV-1-infected chimpanzees were vaccinated with plasmid vaccines, one pCMN160, which expresses envelope glycoprotein HIV-1MN and rev, other control plasmid. chimpanzee immunized pCMN160...
Abstract The murine anti‐APO‐1 antibody (γ3, k) induces programmed cell death (apoptosis) following binding to the APO‐I antigen (m.w., 48 kDa) expressed, e.g., on activated or malignant lymphocytes. expression lines and tissues suggested potential clinical utility supported by anti‐APO‐I‐mediated tumor regression in a nude mouse model. A mouse‐human chimeric with an affinity similar that of was produced. Chimeric showed same inhibit growth SKW6.4 B‐lymphoblastoid line as anti‐APO‐1, In...
Vaccine development strategies have often utilized recombinant envelope glycoproteins which usually generate strong humoral immune responses but do not cytotoxic T lymphyocytes (CTL). A recent novel experimental vaccination approach involves the technology known as nucleic acid immunization in DNA plasmids expressing a gene of interest is injected intramuscularly animals. These expressed proteins then are presented to system with subsequent antibody and cellular (particularly CTL) responses....
The bispecific OC/TR monoclonal antibody (mAb) cross-links the CD3 molecule on T cells with human folate-binding protein (FBP), which is highly expressed nonmucinous ovarian carcinomas. Clinical trials of patients carcinoma mAb have shown some complete and partial responses. Most developed anti-murine immunoglobulin antibodies (HAMA), can inhibit mAb-mediated lysis. We generated a chimeric version to decrease immunogenicity allow more extended treatment schedules. Sp2/0 myeloma were...
Integration of a transposable element adjacent to gene frequently results in an alteration expression the nearby gene. The purpose our experiments was identify cis-acting sequences within yeast transposon (Ty) that are important for role these Ty transcription also analyzed order examine relationship between and expression. Three naturally occurring elements located at HIS4 locus were examined. These differed by multiple sequence changes had different effects on To determine which...
A global effort to control the HIV epidemic is likely rely heavily on immunization strategies. As our closest genetic relative, chimpanzee provides most important model for preclinical safety and immunogenicity studies. We have immunized adult, pregnant infant chimpanzees with plasmid vaccines. found these vaccines be safe well tolerated in all of groups. The same induced both humoral cellular immunity each instance.
Nucleic acid or DNA immunization represents a novel approach to vaccine and immune therapeutic development. The direct injection of expression cassettes into living host results in vivo gene activation. In the case HIV-1 it has been shown by our laboratory that facilitated mimicks aspects live attenuated vaccines both humoral cellular responses can be induced upon nucleic sequence directly target tissue. Antisera from plasmid cassette-immunized animals contain anti-HIV envelope glycoprotein...
The human ovarian carcinoma cell line, IGROV1, produces two forms of folate binding protein (FBP), the membrane form that is anchored to surface by a glycosylphosphatidylinositol tail and soluble shed into tissue culture medium. Both are recognized monoclonal antibodies MOv18 MOv19. Here we describe their purification biochemical characterization. purified appeared as single band with an apparent Mr 36 kDa after SDS-PAGE, whereas 38 kDa. size difference between FBP was confirmed gel...
HCI researchers and practitioners of all backgrounds need to consider the role WEIRD-ness plays in methods, research, communities impact that has on marginalized communities.