A5100324087- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Hepatitis B Virus Studies
- T-cell and B-cell Immunology
- HIV Research and Treatment
- Animal Disease Management and Epidemiology
- Cancer Immunotherapy and Biomarkers
- Viral gastroenteritis research and epidemiology
- Hepatitis C virus research
- Immune Response and Inflammation
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- HIV/AIDS Research and Interventions
- SARS-CoV-2 and COVID-19 Research
- Plant Physiology and Cultivation Studies
- RNA Interference and Gene Delivery
- HIV/AIDS drug development and treatment
- Virus-based gene therapy research
- Respiratory viral infections research
- CAR-T cell therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Asthma and respiratory diseases
- Virology and Viral Diseases
- vaccines and immunoinformatics approaches
- Immune cells in cancer
Fudan University
2015-2025
Baotou Central Hospital
2025
Suzhou Research Institute
2025
Shanghai Medical College of Fudan University
2012-2024
Huashan Hospital
2015-2024
Qingdao University
2022-2024
Peking University
2006-2024
Zhongda Hospital Southeast University
2024
Peking University People's Hospital
2024
Children's Hospital of Fudan University
2018-2023
The coronavirus family member, SARS-CoV-2 has been identified as the causal agent for pandemic viral pneumonia disease, COVID-19. At this time, no vaccine is available to control further dissemination of disease. We have previously engineered a synthetic DNA targeting MERS Spike (S) protein, major surface antigen coronaviruses, which currently in clinical study. Here we build on prior experience generate DNA-based candidate S protein. construct, INO-4800, results robust expression protein...
Tibetans are genetically adapted to high-altitude environments. Though many studies have been conducted, the genetic basis of adaptation remains elusive due poor reproducibility for detecting selective signatures in Tibetan genomes.
Background: Inefficient cellular uptake is a significant limitation to the efficacy of DNA vaccines. In this study, we introduce S-Cr9T, stearyl-modified cell-penetrating peptide (CPP) designed enhance vaccine delivery by forming stable complexes with plasmid DNA, thereby protecting it from degradation and promoting efficient intracellular uptake. Methods Results: vitro studies showed that S-Cr9T significantly improved stability transfection efficiency, optimal performance at an N/P ratio...
Chronic hepatitis B virus (HBV) infection is associated with functionally impaired virus-specific T cell responses. Although the myeloid-derived suppressor cells (MDSCs) are known to play a critical role in impairing antiviral responses, viral factors responsible for expansion of MDSCs chronic (CHB) remain obscure. In order elucidate mechanism monocytic (mMDSCs) and function suppression during persistent HBV infection, we analyzed circulation frequency mMDSCs 164 CHB patients 70 healthy...
With the enormous morbidity and mortality caused by respiratory syncytial virus (RSV) infections among infants elderly, vaccines against RSV are in large market demand.We conducted a first-in-human (FIH), randomized, double-blind, placebo-controlled dose escalation study to evaluate safety immunogenicity response of rRSV vaccine (BARS13) healthy adults aged 18-45. A total 60 eligible participants were randomly assigned receive one four levels or vaccination regimens BARS13 placebo at 4:1...
Abstract: An effective immune response involves the specific recognition of and elimination an infectious organism at multiple levels. In this context DNA immunization can present functional antigenic proteins to host for by all arms system, yet provides opportunity delete any genes which code antigens or pieces that may have deleterious effects. Our group has developed use nucleic acid as a possible method vaccination against Human immunodeficiency virus type 1 (HIV‐1) [1,2,3,10,11,12]....
Abstract: Chemotherapy has been widely used in breast cancer patients to reduce tumor size. However, most anticancer agents cannot differentiate between cancerous and normal cells, resulting severe systemic toxicity. In addition, acquired drug resistance during the chemotherapy treatment further decreases efficacy. With proper strategy, nanodrug carriers, such as liposomes/immunoliposomes, may be able undesired side effects of chemotherapy, overcome multidrug resistance, improve this study,...
Infectious bursal disease (IBD) is an acute, highly contagious, and immunosuppressive avian caused by IBD virus (IBDV). MicroRNAs (miRNAs) are involved in host-pathogen interactions innate immune response to viral infection. However the role of miRNAs host IBDV infection not clear. We report here that gga-miR-155 acts as anti-virus factor inhibiting replication. found transfection DF-1 cells with suppressed replication, while blockage endogenous inhibitors enhanced Furthermore, our data...
Elite non-progressors (plasma viral load <50 copies/ml while antiretroviral naive) constitute a tiny fraction of HIV-infected individuals. After 12 years follow-up cohort 13 long-term (LTNP) identified from 135 individuals with transfusion-acquired HIV infection, 5 remained LTNP after 23 to 26 but only 3 retained elite status. We examined the mechanisms that differentiated delayed progressors in this cohort. A survival advantage was conferred on subjects, who had at least one host genetic...
Herpes simplex virus 1 (HSV-1) spreads in populations through a latency entry and reactivation cycle. The role of host immune-suppressive factor regulatory T cells (Treg cells) controlling establishment is not completely understood. Here, using an HSV-1 ocular infection murine model, we observe positive correlation between the level Treg viral infectivity demonstrate requirement for establishment. Furthermore, show that stress leads to via increased cell control CD8+ cells, permitting...
Although Treg-cell-mediated suppression during infection or autoimmunity has been described, functions of Treg cells highly pathogenic avian influenza virus remain poorly characterized. Here we found that in Foxp3-GFP transgenic mice, CD8(+) Foxp3(+) cells, but not CD4(+) were remarkably induced H5N1 infection. In addition to expressing CD25, the showed a high level GITR and produced IL-10. an adoptive transfer model, suppressed T-cell responses promoted infection, resulting enhanced...
Background: Morphine, a mu-opioid receptor (MOR) agonist commonly utilized in clinical settings alongside chemotherapy to manage chronic pain cancer patients, has exhibited contradictory effects on cancer, displaying specificity toward certain types and doses. Objective: The aim of this study was conduct systematic assessment comparison the impacts morphine three distinct models preclinical setting. Methods: Viability apoptosis assays were conducted panel cell lines following treatment with...
Abstract In situ vaccination (ISV) triggers antitumor immune responses using the patient’s own cancer antigens, yet limited neoantigen release hampers its efficacy. Our novel combination therapy involves low-dose local cisplatin followed by ISV with a TLR7/8/9 agonist formulation (CR108), in which CR108 boosts and sustains induced cisplatin-released neoantigens. mouse models, cisplatin+CR108 significantly outperformed or alone abrogating established 4T1 B16 tumors. The synergistic effects of...
INO-4800 represents a DNA-based vaccine encoding the spike protein of SARS-CoV-2. This phase 2 trial evaluated immunogenicity and safety as primary vaccination series in adults. We conducted randomized, observer-blind, placebo-controlled intradermal injection both healthy adults elderly individuals. Eligible participants from each age group were enrolled randomly assigned 3:3:2 ratio to receive two doses (1.0 mg or 2.0 mg) placebo, followed by electroporation on day 0 28. The endpoints...