A5050460810- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- Plant Pathogens and Fungal Diseases
- Mycotoxins in Agriculture and Food
- Animal Virus Infections Studies
- RNA Interference and Gene Delivery
- HIV Research and Treatment
- Plant Disease Resistance and Genetics
- Biomedical and Engineering Education
- Virus-based gene therapy research
- CAR-T cell therapy research
- Genetics, Bioinformatics, and Biomedical Research
- Viral gastroenteritis research and epidemiology
- Immune Cell Function and Interaction
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Innovative Teaching Methods
- Spectroscopy Techniques in Biomedical and Chemical Research
University of Pennsylvania
2024
The Wistar Institute
2020-2023
ORCID
2021
The coronavirus family member, SARS-CoV-2 has been identified as the causal agent for pandemic viral pneumonia disease, COVID-19. At this time, no vaccine is available to control further dissemination of disease. We have previously engineered a synthetic DNA targeting MERS Spike (S) protein, major surface antigen coronaviruses, which currently in clinical study. Here we build on prior experience generate DNA-based candidate S protein. construct, INO-4800, results robust expression protein...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines may target epitopes that reduce durability or increase the potential for escape from vaccine-induced immunity. Using synthetic vaccinology, we have developed rationally immune-focused SARS-CoV-2 Spike-based vaccines. Glycans can be employed to alter antibody responses infection and Utilizing computational modeling in vitro screening, incorporated glycans into receptor-binding domain (RBD) assessed antigenic profiles. We...
Biomolecular structure drives function, and computational capabilities have progressed such that the prediction design of biomolecular structures is increasingly feasible. Because biophysics attracts students from many different backgrounds with levels resources, teaching subject can be challenging. One strategy to teach diverse learners interactive multimedia material promotes self-paced, active learning. We created a hands-on education set sixteen modules topics in design, fundamentals...
Cancer immunotherapy has demonstrated great promise with several checkpoint inhibitors being approved as the first-line therapy for some types of cancer, and new engineered cytokines such Neo2/15 now evaluated in many studies. In this work, we designed antibody-cytokine chimera (ACC) scaffolding cytokine mimetics on a full-length tumor-specific antibody. We characterized pharmacokinetic (PK) pharmacodynamic (PD) properties first-generation ACC TA99-Neo2/15, which synergized DLnano-vaccines...
Abstract The coronavirus family member, SARS-CoV-2 has been identified as the causal agent for outbreak of viral pneumonia disease, COVID-19 which first emerged in mid-December 2019 city Wuhan central China. As February 25, 2020 there are 80,994 people infected and 2,760 deaths, documented human-to-human transmission across multiple continents. At this time, no vaccine is available to control further dissemination disease. We have previously developed a synthetic DNA targeting MERS Spike (S)...
Abstract HIV Envelope (Env) is the main vaccine target for induction of neutralizing antibodies. Stabilizing Env into native-like trimer (NLT) conformations required recombinant protein immunogens to induce autologous antibodies(nAbs) against difficult neutralize strains (tier-2) in rabbits and non-human primates. Immunizations mice with NLTs have generally failed tier-2 nAbs. Here, we show that DNA-encoded fold properly vivo nAbs mice. also uniquely both CD4 + CD8 T-cell responses as...
Coronavirus disease 2019 (COVID-19) is caused by the newly emerged human coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Due to highly contagious nature of SARS-CoV-2, it has infected more than 137 million individuals and 2.9 deaths globally as April 13, 2021. There an urgent need develop effective novel therapeutic strategies treat or prevent this infection. Toward goal, we focused on development monoclonal antibodies (mAbs) directed against SARS-CoV-2 spike...
Abstract A graduate course on vaccine development challenged students to propose concepts improve upon current paradigms in the context of a “Shark Tank‐style” format where were asked develop an abbreviated business plan and make pitch “Sharks” (experienced academic industrial researchers developers) they could request funding, research collaborations or regulatory guidance. Students graded based components their ability convince feasibility innovation potential project proposals. This...
Abstract Antibodies from SARS-CoV-2 vaccines may target epitopes which reduce durability or increase the potential for escape vaccine-induced immunity. Using a novel synthetic vaccinology pipeline, we developed rationally immune focused Spike-based vaccines. N-linked glycans can be employed to alter antibody responses infection and Utilizing computational modeling comprehensive in vitro screening, incorporated into Spike Receptor-Binding Domain (RBD) assessed antigenic profiles. We glycan...
Abstract Despite extensive efforts, an efficacious HIV vaccine remains elusive. Envelope (Env) is the target of all known neutralizing antibodies. Stabilizing Env into native-like trimer (NLT) conformations required for recombinant protein immunogens to induce autologous antibodies(nAbs) against difficult neutralize strains (tier-2) in rabbits and non-human primates. However, mice are most cost effective widely used model mouse immunizations with NLTs have generally failed tier-2 nAbs. Here,...
Precision agriculture has emerged as a revolutionary technology in helping make farm decisions. It transforms related data into information, and then coverts information useful knowledge for decision-making. This study evaluates economic thresholds new web-based decision support system developed precision fungicide management potato production. Using 10 years of computer simulation experiments from 152 locations the United States, we compared different terms disease severity, usage...