A5030081200- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- DNA Repair Mechanisms
- Hippo pathway signaling and YAP/TAZ
- Chromosomal and Genetic Variations
- Genetic Mapping and Diversity in Plants and Animals
- Plant nutrient uptake and metabolism
- PARP inhibition in cancer therapy
First Affiliated Hospital of Guangzhou Medical University
2023-2025
State Key Laboratory of Respiratory Disease
2022-2025
Guangzhou Medical University
2022-2025
University of Science and Technology of China
2022-2025
Guangzhou Institutes of Biomedicine and Health
2022-2025
Center for Excellence in Molecular Cell Science
2022-2023
Chinese Academy of Sciences
2022-2023
University of Hong Kong
2022
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2022
CTCF mediates chromatin insulation and long-distance enhancer-promoter (EP) interactions; however, little is known about how these regulatory functions are partitioned among target genes in key biological processes. Here, we show that Ctcf expression progressively increased during induced pluripotency. In this process, first as a insulator responsible for direct silencing of the somatic gene program and, interestingly, elevated next ensures accessibility contributes to EP interactions...
Abstract Although extended pluripotent stem cells (EPSCs) have the potential to form both embryonic and extraembryonic lineages, how their transcriptional regulatory mechanism differs from that of (ESCs) remains unclear. Here, we discovered YY1 binds specific open chromatin regions in EPSCs. Yy1 depletion EPSCs leads a gene expression pattern more similar ESCs than control Moreover, triggers series epigenetic crosstalk activities, including changes DNA methylation, histone modifications...
R-loops are prevalent triplex nucleic strands found across various organisms, involved in numerous biological processes. However, the physiological and pathological functions of remain largely unknown due to a lack effective high-resolution detection methods. Here, using nuclease P1, T5 exonuclease, lambda exonuclease mediated digestion ssRNA, ssDNA, dsDNA while preserving RNA:DNA hybrid, we report method named R-loop identification assisted by high-throughput sequencing (RIAN-seq) for...
Abstract Cellular totipotency is critical for whole-organism generation, yet how established remains poorly illustrated. Abundant transposable elements (TEs) are activated in totipotent cells, which embryonic totipotency. Here, we show that the histone chaperone RBBP4, but not its homolog RBBP7, indispensable maintaining identity of mouse stem cells (mESCs). Auxin-induced degradation reprograms mESCs to 2C-like cells. Also, loss RBBP4 enhances transition from trophoblast Mechanistically,...
Abstract The transition of mouse embryonic stem cells (ESCs) between serum/LIF and 2i(MEK GSK3 kinase inhibitor)/LIF culture conditions serves as a valuable model for exploring the mechanisms underlying ground confused pluripotent states. Regulatory networks comprising core ancillary pluripotency factors drive gene expression programs defining stable naïve pluripotency. In our study, we systematically screened essential ESC pluripotency, identifying TEAD2 an factor maintaining ground-state...