A5100334085- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- DNA and Nucleic Acid Chemistry
- Pluripotent Stem Cells Research
- RNA and protein synthesis mechanisms
- Single-cell and spatial transcriptomics
- Land Use and Ecosystem Services
- Enzyme Catalysis and Immobilization
- Influenza Virus Research Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA modifications and cancer
- Oxidative Organic Chemistry Reactions
- Advanced Fiber Laser Technologies
- Virus-based gene therapy research
- Solid State Laser Technologies
- Spectroscopy and Quantum Chemical Studies
- Metal-Catalyzed Oxygenation Mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Genomics and Chromatin Dynamics
- MicroRNA in disease regulation
- Microbial Metabolic Engineering and Bioproduction
- Endoplasmic Reticulum Stress and Disease
- Advanced Biosensing Techniques and Applications
Tianjin Institute of Industrial Biotechnology
2022-2025
Chinese Academy of Sciences
2015-2025
Center for Excellence in Brain Science and Intelligence Technology
2022-2025
University of Chinese Academy of Sciences
2017-2025
Huaihua University
2025
Luoyang Normal University
2020-2025
Chongqing University
2023-2024
Guangzhou Experimental Station
2023-2024
Sun Yat-sen University
2020-2024
Baoding People's Hospital
2024
MicroRNA (miR)-155 is a critical player in both innate and adaptive immune responses. It can influence CD4+ T cell lineage choice. To clarify the role of miR-155 CD25+ regulatory (Treg)/T helper (Th)17 differentiation function, as well mechanism involved, we performed gain-and loss-of-function analysis by transfection pre-miR-155 anti-miR-155 into purified cells. The results showed that positively regulated Treg Th17 differentiation. also induced release interleukin (IL)-17A cells, but not...
Hydroxylation of C25 C–H bonds (referring to sterols) is great importance in vivo for metabolizing sterols and vitamin Ds. The biocatalytic hydroxylation restricted by the selectivity activity enzymes due inertness these bulky compounds. Herein, we employed fungal unspecific peroxygenase from Agrocybe aegerita (AaeUPO) as catalyst develop efficient selective AaeUPO variants through protein engineering. After three rounds evolution using semirational design, 2 variants, G195A/G241V/G318V...
Circular RNAs (circRNA), a subclass of noncoding RNA characterized by covalently closed continuous loops, play emerging roles in tumorigenesis and aggressiveness. However, the functions underlying mechanisms circRNA regulating Wnt/β-catenin signaling cancer progression remain elusive. Here, we screen cis-acting generated β-catenin (CTNNB1)/transcription factor 7-like 2 genes identify one intronic derived from CTNNB1 (circ-CTNNB1) as novel driver progression. Circ-CTNNB1 was predominantly...
The spatial transformation of cultivated land is an important element in the study use transformation. Analyzing characteristics and direction global great significance clarifying extent quality ensuring food security. In this context, paper uses data from 1992 to 2015 analyze perspectives quantity, landscape pattern, management applying Mann-Kendall trend tests, analysis, pattern index measures, rural per capita indicators. area increased, followed by a decrease, during period; there were...
Abstract Genetically modified HSV-1 viruses serve as promising vectors for tumour therapy and vaccine development. The CRISPR/Cas9 system is one of the most powerful tools precise gene editing genomes organisms. However, whether can precisely efficiently make replacements in genome remains essentially unknown. Here, we reported CRISPR/Cas9-mediated human cells, including knockout replacement large genes. In established cells stably expressing CRISPR/Cas9, gRNA coordination with Cas9 could...
Abstract Recent evidence shows the emerging roles of promoter-targeting endogenous microRNAs (miRNAs) in regulating gene transcription. However, miRNAs affecting transcription matrix metalloproteinase 14 ( MMP-14 ) gastric cancer remain unknown. Herein, through integrative mining public datasets, we identified adjacent targeting sites Yin Yang 1 (YY1) and miRNA-584-3p (miR-584-3p) within promoter. We demonstrated that YY1 directly targeted promoter to facilitate its expression cells. In...
CTCF mediates chromatin insulation and long-distance enhancer-promoter (EP) interactions; however, little is known about how these regulatory functions are partitioned among target genes in key biological processes. Here, we show that Ctcf expression progressively increased during induced pluripotency. In this process, first as a insulator responsible for direct silencing of the somatic gene program and, interestingly, elevated next ensures accessibility contributes to EP interactions...
Embryonic stem cells (ESCs) can differentiate into all cell types of the embryonic germ layers. ESCs also generate totipotent 2C-like and trophectodermal cells. However, these latter transitions occur at low frequency due to epigenetic barriers, nature which is not fully understood. Here, we show that treating mouse with sodium butyrate (NaB) increases population enables direct reprogramming trophoblast (TSCs) without a transition through state. Mechanistically, NaB inhibits histone...
Abstract Hemiacetal compounds are valuable building blocks in synthetic chemistry, but their enzymatic synthesis is limited and often hindered by the instability of hemiacetals aqueous environments. Here, we show that this challenge can be addressed through reaction engineering using immobilized peroxygenase from Agrocybe aegerita ( Aae UPO) under neat conditions, which allows for selective C-H bond oxyfunctionalization environmentally significant cyclic ethers to hemiacetals. A wide range...
Epigenetic changes are important considerations for degenerative diseases. DNA methylation regulates crucial genes by epigenetic mechanism, impacting cell function and fate. presents hypermethylation in degenerated nucleus pulposus (NP) tissue, but its role intervertebral disc degeneration (IVDD) remains elusive. This study aimed to demonstrate that methyltransferase mediated was responsible IVDD integrative bioinformatics experimental verification. Methyltransferase DNMT3B highly expressed...
Some RNA species, especially microRNAs, are non-randomly sorted into exosomes, but how selectivity of exosomal sorting is achieved unknown. We found that all three variants RNA-binding ubiquitin E3 ligase (MEX3C)–MEX3C-1, MEX3C-2, and MEX3C-3 –interact with adaptor-related protein complex 2 (AP-2), a cargo adaptor in clathrin-mediated endocytosis. MEX3C's C-terminal RING finger domain the hnRNP K homology (KH) shared by MEX3C both necessary for MEX3C/AP-2 interaction. associates...
Waterlogging is becoming a severe and universal phenomenon in China, so the construction of sponge cities proposed to address urban water problems abiding by overall principles ecological priority low-impact development. The security pattern (ESP) an effective way balance rapid urbanization protection. Taking Zhengzhou as example, this study, performed geographic information system (GIS) modeling, which constructed targeted ESP for city based on comprehensive analysis multi-factor...
Environmental stresses, especially heat and drought, severely limit plant growth negatively affect wheat yield quality worldwide. Heat shock factors (Hsfs) play a central role in regulating responses to various stresses. In this study, the factor gene TaHsfA2e-5D on chromosome 5D was isolated functionally characterized, with goal of investigating its drought Gene expression profiling showed that expressed constitutively tissues, most highly roots at reproductive stage. The up-regulated...
The downstream product transformation of lignin depolymerization is great interest in the production high-value aromatic chemicals. However, this often impeded by chemical oxidation under harsh reaction conditions. In study, we demonstrate that hypohalites generated situ vanadium-containing chloroperoxidase from Curvularia inaequalis (CiVCPO) can halogenate various electron-rich and electron-poor phenol phenolic acid substrates. Specifically, CiVCPO enabled decarboxylative halogenation,...
Abstract Global profile of gene expression at single-cell resolution remains to be determined for primates. Using a recently developed technology (“Stereo-seq”), we have obtained comprehensive spatial transcriptome map the whole-brain level cynomolgus monkeys, with ∼600 genes per cell 10 μm-thick coronal sections (up 15 cm 2 in size). Large-scale single-nucleus RNA-seq analysis ∼1 million cells helped identify types corresponding Stereo-seq profiles, providing 3-D type atlas monkey brain....
Endosomal transport represents the primary mode for intracellular trafficking and signaling transduction thus has to be tightly controlled. The molecular processes controlling endosomal positioning utilize several large protein complexes, one of which contains small GTPase Rab7, Rab-interacting lysosomal (RILP), oxysterol-binding protein–related 1 (ORP1L). Rab7 is known interact with RILP through a canonical binding site termed effector-interacting switch region, but it not clear how...