A5007358576- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Influenza Virus Research Studies
- Enzyme Structure and Function
- Lipid Membrane Structure and Behavior
- Multisensory perception and integration
- Plant Pathogens and Fungal Diseases
- Asymmetric Synthesis and Catalysis
- Microbial Natural Products and Biosynthesis
- Enzyme Catalysis and Immobilization
- Antibiotic Resistance in Bacteria
- Cyclopropane Reaction Mechanisms
- Metabolism, Diabetes, and Cancer
- Plant-Microbe Interactions and Immunity
- Liver Disease Diagnosis and Treatment
- Bone health and osteoporosis research
- Chemical Synthesis and Analysis
- Amino Acid Enzymes and Metabolism
- Visual perception and processing mechanisms
- Nonlinear Dynamics and Pattern Formation
- Phytase and its Applications
- Reliability and Agreement in Measurement
- Heat shock proteins research
North China University of Science and Technology
2023-2025
Microbiology Institute of Shaanxi
2019-2025
Institute of Microbiology
2019-2025
Chinese Academy of Sciences
2016-2025
Kailuan General Hospital
2022-2023
University of Chinese Academy of Sciences
2015-2023
Institute of Biophysics
2015-2023
Czech Academy of Sciences, Institute of Microbiology
2023
The Affiliated Yongchuan Hospital of Chongqing Medical University
2014-2022
Chongqing Medical University
2014-2022
Adaptation of the viral polymerase complex comprising PB1, PB2, and PA is necessary for efficient influenza A virus replication in new host species. We found that mutation K356R (PA-K356R) has become predominant since 2014 avian H9N2 viruses China as with seasonal human H1N1 viruses. The same also most isolates emergent H7N9 H10N8 whose six internal gene segments are derived from virus. further demonstrated mammalian adaptive functionality PA-K356R mutation. Avian A549 cells showed increased...
The structural groups of 2-oxindole and tricyclic 3a-hydroxy-hexahydropyrrolo-[2,3-b]indole (HO-HPI) are important pharmacophores. Chemical synthesis complex alkaloids containing a or HO-HPI moiety, especially the latter one, has been long-standing challenge. Herein, we characterized P450 enzyme AfnD, its homologue proteins, HmtT, ClpD, KtzM, LtzR, as cyclopeptide monooxygenases (cpOPMOs) that could introduce moiety into tryptophan-containing cyclopeptides in pH-dependent manner. A universal...
The triglyceride-glucose (TyG) index is considered as a pivotal factor for various metabolic, cardiovascular, and cerebrovascular diseases. However, there currently paucity of relevant studies on the association between long-term level change TyG-index cardiometabolic diseases (CMDs) risk. We aimed to explore risk CMDs in relation TyG-index.Based prospective cohort study, total 36359 subjects who were free CMDs, had complete data triglyceride (TG) fasting blood glucose (FBG) underwent four...
Abstract Background The Chinese visceral adiposity index (CVAI), a simple surrogate measure of fat, is significantly associated with cardiovascular disease (CVD) risk in the general population. This study aimed to evaluate association cumulative CVAI (cumCVAI) exposure and its accumulation time course CVD among patients hypertension. Methods prospective involved 15,350 hypertension from Kailuan Study who were evaluated at least three times observation period 2006 2014 (2006–2007, 2010–2011,...
Motile bacteria navigate toward favorable conditions and away from unfavorable environments using chemotaxis. Mechanisms of sensing attractants are well understood; however, molecular aspects how sense repellents have not been established. Here, we identified malate as a repellent recognized by the MCP2201 chemoreceptor in bacterium Comamonas testosteroni showed that it binds to same site an attractant citrate. Binding determinants for had only minor differences, single amino acid...
In E. coli and related species, flagellar brake protein YcgR responds to the elevated intracellular c-di-GMP, decreases rotation speed, causes a CCW bias, regulates bacterial swimming. Boehm et al. suggested that c-di-GMP-activated directly interacted with motor MotA curb output. Paul proposed disrupted organization of FliG C-terminal domain bias rotation. The target proteins are controversial, role remains unclear in speed direction regulation by YcgR. Here we assayed proteins' affinity via...
Summary Transmembrane chemoreceptors are widely present in Bacteria and Archaea. They play a critical role sensing various signals outside transmitting to the cell interior. Here, we report structure of periplasmic ligand‐binding domain (LBD) transmembrane chemoreceptor MCP2201, which governs chemotaxis citrate other organic compounds Comamonas testosteroni . The apo‐form LBD crystal revealed typical four‐helix bundle homodimer, similar previously well‐studied such as Tar Tsr Escherichia...
A one-step enzymatic desymmetrization process was developed to produce the direct chiral precursor for S -pregabalin synthesis with high yield and ee p value.
Biofilms are microbial communities that embedded in the extracellular matrix. The exopolysaccharide (EPS) is a key component of biofilm matrix maintains structure and protects bacteria from antimicrobials. Microbial glycoside hydrolases have been exploited to disrupt biofilms by breaking down EPSs. PssZ has recently identified as hydrolase can disperse aggregates Listeria monocytogenes. In this study, crystal determined at 1.6 Å resolution. belongs family 8 adopts classical (α/α)
Purpose To investigate the impact of FPG variability on osteoporotic fractures in entire community population. Methods All participants were from Kailuan Study. Participants completed three consecutive surveys 2006–2007, 2008–2009, and 2010–2011. We excluded individuals with an fracture or prior to index year those without complete records at first 3 examinations. followed date 3rd examination occurrence endpoint event December 31, 2021. According SD levels, included subjects divided into...
Auditory and visual events often happen concurrently, how they group together can have a strong effect on what is perceived. We investigated whether/how intra- or cross-modal temporal grouping influenced the perceptual decision of otherwise ambiguous apparent motion. To achieve this, we juxtaposed auditory gap transfer illusion with Ternus display. The display involves multi-element stimulus that induce either two different percepts motion: 'element motion' (EM) 'group (GM). In "EM," endmost...
The first 1,3‐dipolar cycloadditions (1,3‐DCs) of 1,2‐disubstituted alkynes with aldehyde‐generated azomethine ylides have been established, leading to the efficient synthesis poly‐substituted 2,5‐dihydropyrroles.The Brønsted acid‐catalyzed three‐component 1,3‐DCs but‐2‐ynedioates, aldehydes, and diethyl 2‐aminomalonate tolerate a wide range substrates, offering structurally diverse 2,5‐dihydropyrroles in high yields. This protocol not only provides an easy approach but also greatly enriches...
RNase J is a prokaryotic 5′-3′ exo/endoribonuclease that functions in mRNA decay and rRNA maturation. Here, we report novel duplex unwinding activity of mpy-RNase J, an archaeal from Methanolobus psychrophilus, which enables it to degrade RNAs with hairpins up 40 bp when linking 5′ single-stranded overhangs ≥ 7 nt, corresponding the RNA channel length. A 6-nt RNA-mpy-RNase J-S247A structure reveals RNA-interacting residues steric barrier at entrance comprising two loops helices. Mutagenesis...
Crossmodal correspondences are the automatic associations that most people have between different basic sensory stimulus attributes, dimensions, or features. For instance, often show a systematic tendency to associate moving objects with changing pitches. Cognitive styles defined as an individual's consistent approach think, perceive, and remember information, they reflect qualitative rather than quantitative differences individuals in their thinking processes. Here we asked whether...
Abstract Motile bacteria navigate toward favorable conditions and away from unfavorable environments using chemotaxis. Mechanisms of sensing attractants are well understood, however molecular aspects how sense repellents have not been established. Here, we identified malate as a repellent recognized by the MCP2021 chemoreceptor in bacterium Comamonas testosteroni showed that it binds to same site an attractant citrate. Binding determinants for had only minor differences, single amino acid...
NS1-binding protein (NS1-BP), which belongs to the Kelch superfamily, was first identified as a novel human 70 kDa that interacts with NS1 of Influenza A virus . It is involved in many cell functions, including pre-mRNA splicing, ERK signalling pathway, aryl hydrocarbon receptor (AHR) F-actin organization and ubiquitylation. However, structure NS1-BP still unknown, may impede functional studies. Here, C-terminal domain (NS1-BP-C; residues 330–642) determined at 1.98 Å resolution. The adopts...