A5112400806- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Carbohydrate Chemistry and Synthesis
- Synthetic Organic Chemistry Methods
- Biochemical and Molecular Research
- Fungal Biology and Applications
- Wheat and Barley Genetics and Pathology
- Microbial Metabolic Engineering and Bioproduction
- Plant biochemistry and biosynthesis
- Enzyme Catalysis and Immobilization
- Enzyme Structure and Function
- Phytochemical compounds biological activities
- Plant Disease Resistance and Genetics
- Oral microbiology and periodontitis research
- Probiotics and Fermented Foods
- Marine Sponges and Natural Products
- Bacterial Genetics and Biotechnology
- Gut microbiota and health
- Genetics and Plant Breeding
- Microbial Metabolism and Applications
- CRISPR and Genetic Engineering
- Enzyme Production and Characterization
- Antifungal resistance and susceptibility
- Alkaloids: synthesis and pharmacology
- Gene Regulatory Network Analysis
University of Chinese Academy of Sciences
2016-2025
Taichung Veterans General Hospital
2025
Microbiology Institute of Shaanxi
2016-2025
Institute of Microbiology
2016-2025
Chinese Academy of Sciences
2016-2025
Providence University
2024-2025
Northwestern University
2000-2025
Central South University
2025
University of Hong Kong - Shenzhen Hospital
2023-2025
University of Hong Kong
2023-2025
Platensimycin (PTM) and platencin (PTN) are potent selective inhibitors of bacterial mammalian fatty acid synthases have emerged as promising drug leads for both antibacterial antidiabetic therapies. Comparative analysis the PTM PTN biosynthetic machineries in Streptomyces platensis MA7327 MA7339 revealed that divergence biosynthesis is controlled by dedicated ent -kaurene -atiserene synthases, latter which represents a new pathway diterpenoid biosynthesis. The provide rare glimpse at how...
A beneficial gut Bacteroides-folate-liver pathway regulating lipid metabolism is demonstrated. Oral administration of a Ganoderma meroterpene derivative (GMD) ameliorates nonalcoholic hepatic steatosis in the liver fa/fa rats by reducing endotoxemia, enhancing oxidation, decreasing de novo lipogenesis, and suppressing export from liver. An altered microbiota with an increase butyrate folate plays causative role effects GMD. The commensal bacteria Bacteroides xylanisolvens, thetaiotaomicron,...
Genetic diversity is a result of evolution, enabling multiple ways for one particular physiological activity. Here, we introduce this strategy into bioengineering. We design two hydroxytyrosol biosynthetic pathways using tyrosine as substrate. show that the synthetic capacity significantly improved when work simultaneously comparing to each individual pathway. Next, engineer flavin-dependent monooxygenase HpaBC tyrosol hydroxylase, tyramine and promiscuous hydroxylase active on both directed...
Intercellular signaling is indispensable for single cells to form complex biological structures, such as biofilms, tissues and organs. The genetic tools available engineering intercellular signaling, however, are quite limited. Here we exploit the chemical diversity of small molecules de novo design a toolbox high-performance, multi-channel cell-cell communications computations. By biosynthetic pathway signal molecules, rational sensing promoters directed evolution transcription factors,...
Akkermansia muciniphila is a gram-negative bacterium that colonizes the human gut, making up 3–5% of microbiome. A. promising next-generation probiotic with clinical application prospects. Emerging studies have reported various beneficial effects including anti-cancer, delaying aging, reducing inflammation, improving immune function, regulating nervous system whereas knowledge on its roles and mechanism in infectious disease currently unclear. In this review, we summarized basic...
Abstract Diterpene cyclases from bacteria and basidiomycete fungi are seldom studied. Here, we presented the identification verification of EriG, a member UbiA superfamily, as enzyme responsible for cyclization cyathane skeleton in mushroom Hericium erinaceum . Genome mining using EriG protein sequence probe led to discovery new family ubiquitous UbiA‐related diterpene fungi. We successfully characterized seven or with help an engineered Escherichia coli strain determined structures their...
Abstract Streptococcus mutans is a major pathogen causing human dental caries. As Gram-positive bacterium with small genome (about 2 Mb) it considered poor source of natural products. Due to recent explosion in genomic data available for S. strains, we were motivated explore the product production potential this organism. Bioinformatic characterization 169 publically genomes from caries revealed surprisingly rich biosynthetic gene clusters. Anti-SMASH analysis identified one nonribosomal...
The fredericamycin (FDM) A biosynthetic gene cluster, cloned previously from Streptomyces griseus ATCC 49344, contains three putative regulatory genes, fdmR, fdmR1, and fdmR2. Their deduced products show high similarity to members of the antibiotic protein (SARP) family (FdmR1) or MarR-like regulators (FdmR FdmR2). Here we provide experimental data supporting FdmR1 as a SARP-type activator. Inactivation fdmR1 abolished FDM biosynthesis, production could be restored fdmR1::aac(3)IV mutant by...
Anaerobic bacteria represent an overlooked rich source of biological and chemical diversity. Due to the challenge cultivation genetic intractability, assessing capability their biosynthetic gene clusters (BGCs) for secondary metabolite production requires efficient heterologous expression system. However, this kind host system is still unavailable. Here, we use facultative anaerobe Streptococcus mutans UA159 as a BGCs from anaerobic bacteria. A natural competence based large DNA fragment...
Significance Septacidin and its analogs are potential anticancer pain-relief drugs. Hygromycin B is an anthelmintic agent practically used in swine poultry farming. A common feature of these compounds that they all have heptose moieties. Here we show the heptoses septacidin hygromycin both derived from d -sedoheptulose-7-phosphate but biosynthesized through different pathways. producer, a gram-positive bacterium, shares same ADP-heptose biosynthesis pathway with gram-negative bacterium...
Bacterial small molecule metabolites such as adenosine-diphosphate-d-
A novel angucycline metabolite, 2,3-dehydro-UWM6, was identified in a jadH mutant of Streptomyces venezuelae ISP5230. Both UWM6 and 2,3-dehydro-UWM6 could be converted to jadomycin or B by ketosynthase alpha (jadA) S. venezuelae. These intermediates were also transformant the heterologous host lividans expressing jadFGH oxygenases vivo its cell-free extracts vitro; thus three gene products JadFGH are implicated catalysis post-polyketide synthase biosynthetic reactions converting aglycone....
Enediynes are potent antitumor antibiotics that classified as 9- or 10-membered according to the size of enediyne core structure. However, almost nothing is known about biosynthesis, and determinants versus biosynthetic divergence remain elusive. Previous work identified enediyne-specific polyketide synthases (PKSEs) can be phylogenetically distinguished being involved in suggesting might originate from differing PKSE chemistries. Recent vitro studies have several compounds produced by...
iso-Migrastatin and related glutarimide-containing polyketides are potent inhibitors of tumor cell migration their implied potential as antimetastatic agents for human cancers has garnered significant attention. Genome scanning Streptomyces platensis NRRL 18993 unveiled two candidate gene clusters (088D mgs); each encodes acyltransferase-less type I polyketide synthases commensurate with iso-migrastatin biosynthesis. Both were inactivated by lambda-RED-mediated PCR-targeting mutagenesis in...
ABSTRACT Yellow rust, also known as stripe is caused by Puccinia striiformis Westend. f. sp. tritici Eriks (PST) and one of the most common persistent wheat ( Triticum aestivum L.) diseases worldwide. A mapping population recombinant inbred lines from cross ‘Jagger’ (moderately resistant) × ‘2174’ susceptible) was tested at three sites in Washington where predominant races PST‐114 PST‐116 naturally occurred, Rossville, KS, PST‐100 inoculated, Beijing, China, a Chinese rust race CYR32...
Abstract ADP-activated β - d manno -heptoses (ADP- -heptoses) are precursors for the biosynthesis of inner core lipopolysaccharide in Gram-negative bacteria. Recently, ADP- glycero -heptose , -heptose) and its C-6′′ epimer, l -heptose), were identified as potent pathogen-associated molecular patterns (PAMPs) that can trigger robust innate immune responses. Although production has been studied several different pathogenic bacteria, current knowledge Vibrio strains remains limited. Here, we...
Backbone-breaking oxygenase clusters. Inactivation of the oxygenase-encoding genes jadH and jadG structure elucidation novel accumulation products illustrate essential interactions within biosynthetic protein complex that govern oxygenation cascade leading to cleavage angucycline backbone during jadomycin biosynthesis by Streptomyces venezuelae. These results also allow dehydration sequence (shown) yields aromatic ring A scaffold.