A5101949564- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Streptococcal Infections and Treatments
- Bacterial Genetics and Biotechnology
- Antimicrobial Resistance in Staphylococcus
- Microbial Metabolic Engineering and Bioproduction
- Oral microbiology and periodontitis research
- RNA and protein synthesis mechanisms
- Marine Sponges and Natural Products
- Infective Endocarditis Diagnosis and Management
- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Biofuel production and bioconversion
- Fungal and yeast genetics research
- Bacterial biofilms and quorum sensing
- Neonatal and Maternal Infections
- Microbial Metabolism and Applications
- Enzyme Production and Characterization
- CRISPR and Genetic Engineering
- Microbial Community Ecology and Physiology
- Proteoglycans and glycosaminoglycans research
- Biochemical and Molecular Research
- Cocoa and Sweet Potato Agronomy
- Seaweed-derived Bioactive Compounds
- Microbial metabolism and enzyme function
Tianjin University of Science and Technology
2017-2025
Microbiology Institute of Shaanxi
2007-2020
Institute of Microbiology
2007-2020
Chinese Academy of Sciences
2007-2020
State Key Laboratory of Microbial Resources
2007-2020
University of Oklahoma Health Sciences Center
2010-2015
Shanghai Institutes for Biological Sciences
2012
Center for Excellence in Molecular Cell Science
2012
University of Oklahoma
2010
Insertion duplication mutagenesis and allelic replacement are among the most commonly utilized approaches for targeted in bacteria. However, both techniques limited by a variety of factors that can complicate mutant phenotypic studies. To circumvent these limitations, multiple markerless have been developed utilize either temperature-sensitive plasmids or counterselectable suicide vectors containing positive- negative-selection markers. For many species, not especially useful due to...
Abstract Streptococcus mutans is a major pathogen causing human dental caries. As Gram-positive bacterium with small genome (about 2 Mb) it considered poor source of natural products. Due to recent explosion in genomic data available for S. strains, we were motivated explore the product production potential this organism. Bioinformatic characterization 169 publically genomes from caries revealed surprisingly rich biosynthetic gene clusters. Anti-SMASH analysis identified one nonribosomal...
A gene cluster (pol) essential for the biosynthesis of polyoxin, a nucleoside antibiotic widely used control phytopathogenic fungi, was cloned from Streptomyces cacaoi. 46,066-bp region sequenced, and 20 39 putative open reading frames were defined as necessary polyoxin evidenced by its production in heterologous host, lividans TK24. The role PolO PolA synthesis demonstrated vivo experiments, their functions unambiguously characterized O-carbamoyltransferase UMP-enolpyruvyltransferase,...
Anaerobic bacteria represent an overlooked rich source of biological and chemical diversity. Due to the challenge cultivation genetic intractability, assessing capability their biosynthetic gene clusters (BGCs) for secondary metabolite production requires efficient heterologous expression system. However, this kind host system is still unavailable. Here, we use facultative anaerobe Streptococcus mutans UA159 as a BGCs from anaerobic bacteria. A natural competence based large DNA fragment...
Significance Septacidin and its analogs are potential anticancer pain-relief drugs. Hygromycin B is an anthelmintic agent practically used in swine poultry farming. A common feature of these compounds that they all have heptose moieties. Here we show the heptoses septacidin hygromycin both derived from d -sedoheptulose-7-phosphate but biosynthesized through different pathways. producer, a gram-positive bacterium, shares same ADP-heptose biosynthesis pathway with gram-negative bacterium...
Recently, we described the function of an uncharacterized two-gene regulatory system consisting a LytTR family transcription regulator and putative membrane protein, which referred to as hdrRM operon. In this study, determined that HdrRM controls expression analogous annotated SMU.2080 SMU.2081. Like hdrRM, SMU.2080-2081 operon encodes now refer BrsR BrsM respectively. Examination mechanism BrsRM suggests serves antagonize BrsR. Further analyses role it functions activator for variety...
The Streptococcus mutans hdrRM operon encodes a novel two-gene regulatory system induced by high cell density. Previous studies identified hdrM as the only known negative regulator of competence development in S. mutans. In present study, we demonstrated that HdrRM bypasses prototypical gene regulators ComC and ComDE transcriptional regulation competence-specific sigma factor comX late genes. Similarly, can abrogate requirement for ComE to produce bacteriocin mutacin IV. To further probe...
Previous studies have identified the hdrRM operon as a novel regulatory system induced by conditions of high cell density. Little is known about genes under control this system, but variety important phenotypes are associated with either hdrR overexpression or mutation hdrM. To characterize function HdrRM in Streptococcus mutans we used microarray approach to compare transcriptional profiles an strain hdrM mutant. Both strains exhibited almost identical profiles, which included all late...
Establishing the pattern of expression transmitters and peptides as well their receptors in different neuronal types is crucial for understanding circuitry various regions brain. Previous studies have demonstrated that transmitter peptide phenotypes mouse dorsal spinal cord neurons are determined by transcription factors <i>Tlx1/3</i> <i>Ptf1a</i>. Here we show these also determine two distinct sets receptor genes this region. We screened 78 horn <i>in situ</i> hybridization. found...
Hyaluronic acid (HA) is extensively employed in various fields such as medicine, cosmetics, food, etc. The molecular weight (MW) of HA crucial for its biological functions. Streptococcus zooepidemicus, a prominent industrial producer, naturally synthetizes with high MW. Currently, few effective approaches exist the direct and precise regulation MW through one-step fermentation process, S. zooepidemicus lacks metabolic regulatory elements varying intensities. ratio HA's precursors,...
The polyoxin (POL) biosynthetic gene cluster ( pol ) was recently cloned from Streptomyces cacaoi subsp. asoensis . A 3.3 kb DNA fragment carrying an obvious open reading frame polR ), whose deduced product shows sequence similarity to SanG of ansochromogenes and PimR natalensis , revealed within the cluster. Disruption abolished POL production, which could be complemented by integration a single copy into chromosome non-producing mutant. introduction extra in wild-type strain resulted...
polY, a transcriptional regulatory gene in the polyoxin biosynthetic cluster of Streptomyces cacaoi, was analysed, and its deduced product (PolY) showed amino acid sequence homology to AfsR from coelicolor A3(2). PolY contains an OmpR-like DNA binding domain at N-terminal ATPase middle protein. Disruption polY abolished biosynthesis, which could be restored by integration single copy into chromosome disruption mutant. Transcription polR, pathway-specific controlled polY. Electrophoretic...
Despite the plethora of genetic tools that have been developed for use in Streptococcus mutans, S. mutans system still lacks an effective gene induction exhibiting low basal expression and strong inducibility. Consequently, we created two hybrid cassettes referred to as Xyl-S1 Xyl-S2. Both Xyl-S are xylose inducible controlled by Bacillus megaterium repressor. The each demonstrated >600-fold-increased reporter activity presence 1.2% (wt/vol) xylose. However, cassette yielded a much higher...
The paulomycins are a group of glycosylated compounds featuring unique paulic acid moiety. To locate their biosynthetic gene clusters, the genomes two paulomycin producers, Streptomyces paulus NRRL 8115 and sp. YN86, were sequenced. clusters defined by comparative analyses together with genome third producer albus J1074. Subsequently, identity cluster was confirmed inactivation genes involved in biosynthesis paulomycose branched chain (pau11) ring A moiety (pau18) 8115. After determining...
The most commonly studied prokaryotic sensory signal transduction systems include the one-component systems, phosphosignaling extracytoplasmic function (ECF) sigma factor and various types of second messenger systems. Recently, we described regulatory role two separate in Streptococcus mutans that jointly control bacteriocin gene expression, natural competence development, as well a cell death pathway, yet they do not via any currently recognized paradigms. These which refer to LytTR...
Summary Streptomyces coelicolor produces a brown pigment on nutrient‐limited agar medium (Tyr‐PM) using l ‐tyrosine as the sole nitrogen and carbon source. The production is associated with second step of catabolism catalysed by 4‐hydroxyphenylpyruvate dioxygenase (HppD), which converts (4HPP) to 2, 5‐dihydroxyphenylacetate (homogentisate) provide energy substrates for growth S. Tyr‐PM. An hppD mutant did not produce pigment, its normal was impaired hpdA hpdR , located close were identified...
Streptococcus zooepidemicus is an important opportunistic pathogen of several species including humans. This organism also well known as the main producing strain in industrial production hyaluronic acid (HA), which component its capsule polysaccharide. How virulence and polysaccharide regulated remains poorly understood. Intercellular chemical signaling among bacteria provides communities microbes opportunity to coordinate gene expression facilitate group behavior, such pathogenicity,...
Citric acid is widely used in the food, chemical and pharmaceutical industries. Aspergillus niger workhorse for citric production industry. A canonical citrate biosynthesis that occurred mitochondria was well established; however, some research suggested cytosolic pathway may play a role this production. Here, roles of phosphoketolase (PK), acetate kinase (ACK) acetyl-CoA synthetase (ACS) were investigated by gene deletion complementation A. niger. The results indicated PK, ACK ACS important...
Kojic acid (KA) is a widely used compound in the cosmetic, medical, and food industries, typically produced by Aspergillus oryzae. To meet increasing market demand, it important to optimize KA production through seeking alternatives that are more economic than current A. oryzae-based methods.In this study, we achieved first successful heterologous of niger, an industrially fungus does not naturally produce KA, expression kojA gene from Using resulting KA-producing niger strain as platform,...