A5109314544- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Carbohydrate Chemistry and Synthesis
- RNA and protein synthesis mechanisms
- Microbial Metabolism and Applications
- Plant-Microbe Interactions and Immunity
- Marine Sponges and Natural Products
- Biochemical and Molecular Research
- Plant Disease Resistance and Genetics
- Enzyme Production and Characterization
- Synthetic Organic Chemistry Methods
- Bacteriophages and microbial interactions
- Microbial Community Ecology and Physiology
- Plant biochemistry and biosynthesis
- Probiotics and Fermented Foods
- Protist diversity and phylogeny
- Peptidase Inhibition and Analysis
- Tea Polyphenols and Effects
- Chemical Synthesis and Analysis
- Fungal Biology and Applications
- Metal-Catalyzed Oxygenation Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Enterobacteriaceae and Cronobacter Research
Microbiology Institute of Shaanxi
2016-2025
Chinese Academy of Sciences
2016-2025
University of Chinese Academy of Sciences
2017-2025
Institute of Microbiology
2016-2025
Anhui Normal University
2024
Xuzhou Medical College
2023-2024
Anhui Agricultural University
2006-2019
Hefei University
2015
Drew University
2009
State Key Laboratory of Microbial Resources
2005-2007
Phenolic compounds in tea plant [Camellia sinensis (L.)] play a crucial role dominating flavor and possess number of key pharmacological benefits on human health. The present research aimed to study the profile tissue-specific, development-dependent accumulation pattern phenolic plant. A total 50 were identified qualitatively using liquid chromatography tandem mass spectrometry technology. Of which 29 quantified based their fragmentation behaviors. Most higher younger leaves than that stem...
Thermoanaerobacter tengcongensis is a rod-shaped, gram-negative, anaerobic eubacterium that was isolated from freshwater hot spring in Tengchong, China. Using whole-genome-shotgun method, we sequenced its 2,689,445-bp genome an isolate, MB4 T (Genbank accession no. AE008691 ). The encodes 2588 predicted coding sequences (CDS). Among them, 1764 (68.2%) are classified according to homology other documented proteins, and the rest, 824 CDS (31.8%), functionally unknown. One of interesting...
In bacteria, many "atypical" response regulators (ARRs) lack the conserved residues important for phosphorylation by which typical switch their output response, suggesting existence of alternative regulatory mechanisms. However, such mechanisms have not been established. JadR1, an OmpR-type ARR Streptomyces venezuelae, appears to activate transcription jadomycin B (JdB) biosynthetic genes while repressing its own gene. JadR1 activities were inhibited in cells induced produce JdB, was found...
In actinomycetes, the onset of secondary metabolite biosynthesis is often triggered by quorum-sensing signal gamma-butyrolactones (GBLs) via specific binding to their cognate receptors. However, presence multiple putative GBL receptor homologues in genome suggests existence an alternative regulatory mechanism. Here, model streptomycete Streptomyces coelicolor, ScbR2 (SCO6286, a homologue receptor) shown not bind endogenous molecule SCB1, hence designated "pseudo" receptor. Intriguingly, it...
In the present study, proanthocyanidins were qualitatively and quantitatively identified using hydrolysis thiolysis assays, NP-HPLC, HPLC-ESI-MS, MALDI-TOF-MS, (1)H-NMR, (13)C-NMR techniques in different organs of tea plants. The results showed that leaves, tri-hydroxyl, cis- galloylated flavan-3-ols main monomeric catechins units, (epi)catechin was found to be major unit polymeric when degree polymerization greater than five. roots, PAs abundant, epicatechin formed predominant extension...
Several strategies have been used to clone large DNA fragments directly from bacterial genome. Most of these approaches are based on different site-specific recombination systems consisting a specialized recombinase and its target sites. In this study, novel strategy phage ϕBT1 integrase-mediated was developed, for simultaneous Streptomyces genome engineering cloning antibiotic gene clusters. This method has proved successful the actinorhodin cluster coelicolor M145, napsamycin daptomycin...
Genome sequencing analysis has revealed at least 35 clusters of likely biosynthetic genes for secondary metabolites in Streptomyces ansochromogenes. Disruption adpA encoding a global regulator (AdpA) resulted the failure nikkomycin production, whereas other antibacterial activities against Staphylococcus aureus, Bacillus cereus, and subtilis were observed with fermentation broth ΔadpA but not that wild-type strain. Transcriptional showed cryptic gene cluster (pks7), which shows high identity...
Two regulatory genes, jadR2 and jadR3, in the jadomycin (jad) biosynthetic gene cluster of Streptomyces venezuelae encode homologues γ-butyrolactone receptor. JadR2 was previously shown to be a pseudo jadR3 is situated at upstream jadW123 encoding putative enzymes for biosynthesis. Disruption resulted markedly decreased production jadomycin. Transcriptional analysis revealed that JadR3 represses jadW1, but activates jadR1, key activator DNase I footprinting showed has four binding sites...
Summary The plant flavonoid dogma proposes that labile carbocations (PFCs) play vital roles in the biosynthesis of proanthocyanidins (PAs). However, whether PFCs exist plants and how function remain unclear. Here, we report use an integrative strategy including enzymatic assays, mutant analysis, metabolic engineering, isotope labeling profiling to capture demonstrate their functions. In anthocyanidin reductase (ANR) (−)‐epicatechin conjugate was captured protic polar nucleophilic methanol...
Summary DNA sequence analysis of a 7.5 kb Xho I fragment from the region flanking nikkomycin biosynthesis gene cluster in Streptomyces ansochromogenes revealed one 3.3 open reading frame (ORF), designated sanG . The deduced product (1061 amino acids), which is similar to PimR natalensis , contains an OmpR‐like binding domain its N‐terminal portion and A‐ B‐type nucleotide motifs middle protein. Disruption abolished biosynthesis, reduced sporulation led brown pigment accumulation. All aspects...
A gene cluster (pol) essential for the biosynthesis of polyoxin, a nucleoside antibiotic widely used control phytopathogenic fungi, was cloned from Streptomyces cacaoi. 46,066-bp region sequenced, and 20 39 putative open reading frames were defined as necessary polyoxin evidenced by its production in heterologous host, lividans TK24. The role PolO PolA synthesis demonstrated vivo experiments, their functions unambiguously characterized O-carbamoyltransferase UMP-enolpyruvyltransferase,...
Many taxonomically diverse prokaryotes enzymatically modify their DNA by replacing a non-bridging oxygen with sulfur atom at specific sequences. The biological implications of this S-modification (phosphorothioation) were unknown. We observed that simultaneous expression the dndA-E gene cluster from Streptomyces lividans 66, which is responsible for S-modification, and putative coelicolor A(3)2 Type IV methyl-dependent restriction endonuclease ScoA3McrA (Sco4631) leads to cell death in same...
A novel two-component system (TCS) designated as DraR-K (sco3063/sco3062) was identified to be involved in differential regulation of antibiotic biosynthesis Streptomyces coelicolor. The S. coelicolor mutants with deletion either or both draR and draK exhibited significantly reduced actinorhodin (ACT) but increased undecylprodigiosin (RED) production on minimal medium (MM) supplemented separately high concentration different nitrogen sources. These also overproduced a yellow-pigmented type I...
Abstract Zinc oxide nanoparticles (ZnO NPs) stand as among the most significant metal in trigger formation of reactive oxygen species (ROS) and induce apoptosis. Nevertheless, utilization ZnO NPs has been limited by shallowness short-wavelength light constrained production ROS. To overcome these limitations, a strategy involves achieving red shift towards near-infrared (NIR) spectrum, promoting separation restraining recombination electron-hole (e − -h + ) pairs. Herein, hybrid plasmonic...
Abstract Cuproptosis, a recently discovered copper‐dependent cell death, presents significant potential for the development of copper‐based nanoparticles to induce cuproptosis in cancer therapy. Herein, unique ternary heterojunction, denoted as HACT, composed core–shell Au@Cu 2 O nanocubes with surface‐deposited Titanium Dioxide quantum dots and modified hyaluronic acid is introduced. Compared AC NCs, TiO /Au@Cu exhibits improved energy structure optimization, successfully separating...
The nikkomycin-producing strain Streptomyces ansochromogenes has a homologue (adpA-L) of the key pleiotropic regulatory gene adpA. Gene disruption and genetic complementation revealed that adpA-L was required for both nikkomycin biosynthesis morphological differentiation. Transcriptional analysis suggested transcription sanG, specific activator biosynthesis, dependent on AdpA-L. In gel-shift DNase 1 footprinting assays, purified His(6)-tagged recombinant AdpA-L protein bound upstream region...
In streptomycetes, a quorum-sensing mechanism mediated by γ-butyrolactones (GBLs) and their cognate receptors was known to trigger secondary metabolism morphological differentiation. However, many aspects on the control of GBL signal production are not understood. this work, we report that ScbR2, pseudo receptor in Streptomyces coelicolor, negatively controls biosynthesis γ-butyrolactone (SCB1) directly repressing transcription scbA, which encodes key enzyme for SCB1 biosynthesis. Similarly,...
Marine microorganisms are an important source of new drug leads. However, the discovery and sustainable production these compounds often hampered due to unavailable expression cryptic biosynthetic gene clusters or limited titer. Ribosome engineering response surface methodology (RSM) integrated strategy was developed in this study activate cluster deepsea-derived Streptomyces somaliensis SCSIO ZH66, subsequently isolation, structural analysis, yield enhancement activated compound, anticancer...
Nikkomycins are a group of peptidyl nucleoside antibiotics produced by Streptomyces ansochromogenes. They competitive inhibitors chitin synthase and show potent fungicidal, insecticidal, acaricidal activities. Nikkomycin X Z the main components S. Generation high-producing strain is crucial to scale up nikkomycins production for further clinical trials.