- Tea Polyphenols and Effects
- Plant Gene Expression Analysis
- Phytochemicals and Antioxidant Activities
- Plant biochemistry and biosynthesis
- Food Quality and Safety Studies
- Catalytic Processes in Materials Science
- Fluorine in Organic Chemistry
- Catalysis and Oxidation Reactions
- Biochemical and biochemical processes
- Photosynthetic Processes and Mechanisms
- Marine Sponges and Natural Products
- Heavy Metals in Plants
- Chemical synthesis and alkaloids
- Synthetic Organic Chemistry Methods
- GABA and Rice Research
- Genomics, phytochemicals, and oxidative stress
- Nanomaterials for catalytic reactions
- Plant Molecular Biology Research
- Ammonia Synthesis and Nitrogen Reduction
- Fermentation and Sensory Analysis
- Inorganic Fluorides and Related Compounds
- Cancer Treatment and Pharmacology
- Biological Activity of Diterpenoids and Biflavonoids
- Microbial Metabolism and Applications
- Metal-Organic Frameworks: Synthesis and Applications
Qingdao Agricultural University
2019-2025
Ministry of Agriculture and Rural Affairs
2023-2024
Anhui Agricultural University
2017-2020
Heilongjiang University
2019-2020
Harbin University
2020
Ministry of Education of the People's Republic of China
2019
East China University of Science and Technology
2015-2017
Drying is an important factor in the formation of green tea flavor. In this study, metabolomic analysis were used to detect characteristic components four teas with clean aroma (CA), chestnut-like (CLA), bean-like (BLA) and high-fired (HFA) produced by adjusting drying temperature. The sensory evaluation results showed that dried at 90°C, 110°C, 140°C 160°C had a aroma, respectively. A total 95 volatile compounds identified. Linalool, naphthalene, 2-methyl-naphthalene, 1-octen-3-ol...
Flavan-3-ols and oligomeric proanthocyanidins (PAs) are the main nutritional polyphenols in green tea (Camellia sinensis), which provide numerous benefits to human health. To date, regulatory mechanism of flavan-3-ol biosynthesis remains open study. Herein, we report characterization a C. sinensis tryptophan-aspartic acid repeat protein (CsWD40) that interacts with myeloblastosis (MYB) basic helix-loop-helix (bHLH) transcription factors (TFs) regulate flavan-3-ols. Full length CsWD40 cDNA...
LARs promoted the biosynthesis of catechin monomers and inhibited their polymerization. The accumulation polymers was increased by up-regulating expression NtLAR NtANR s in CsMYB5b transgenic tobacco. Tea is rich polyphenolic compounds, catechins are major polyphenols tea. closely related to leucoanthocyanidin reductase (LAR) anthocyanidin (ANR) genes. In this paper, an evolutionary analysis functional characterization three CsLARs were performed. phylogenetic tree showed that plant could be...
Salicylic acid (SA) is an important plant hormone and signal required for establishing resistance to diverse pathogens diseases. The abundant polyphenols in tea plants also defend from biotic abiotic stresses. However, whether exogenous SA would increase the of adversity relationship between are still poorly understood. Here, we carried out treatment on seedlings performed transcriptome sequencing. inhibited phenylpropanoid flavonoid metabolic pathways but promoted lignin pathways. increased...
Summary The plant flavonoid dogma proposes that labile carbocations (PFCs) play vital roles in the biosynthesis of proanthocyanidins (PAs). However, whether PFCs exist plants and how function remain unclear. Here, we report use an integrative strategy including enzymatic assays, mutant analysis, metabolic engineering, isotope labeling profiling to capture demonstrate their functions. In anthocyanidin reductase (ANR) (−)‐epicatechin conjugate was captured protic polar nucleophilic methanol...
Melatonin and gibberellin are bioactive molecules in plants. In the present study, role of exogenous melatonin (MT) (GA) tea plant was explored by transcriptome metabolic analysis. Results showed that growth enhanced MT treatment. The pathways terpenoid synthesis plant-pathogen interaction were significantly strengthened, combined with upregulation LRR-RLK transcription factors which contributed to plant. internode elongation leaf enlargement hastened GA Significantly modulated expression...
Flavonoids are major secondary metabolites in Camellia sinensis. Flavanone-3-hydroxylase (F3H) is a key enzyme flavonoid biosynthesis plants. However, its role the metabolism C. sinensis has not been well studied. In this study, we cloned two F3Hs from sinensis, named CsF3Ha and CsF3Hb, where containing 1107 bases encoded 368 amino acids, CsF3Hb 1071 357 acids. Enzymatic activity analysis showed both recombinant CsF3H enzymes Escherichia coli could convert naringenin eriodictyol into...
Abstract Flavonol glycosides, which are often converted from aglycones in a process catalyzed by UDP-glycosyltransferases (UGTs), play an important role for the health of plants and animals. In present study, gene encoding flavonoid 7- O -glycosyltransferase (CsUGT75L12) was identified tea plants. Recombinant CsUGT75L12 protein displayed glycosyltransferase activity on 7-OH position multiple phenolic compounds. relative comparison to wild-type seeds, levels flavonol-glucosides increased...
Chestnut-like aroma is one of the unique qualities Chinese green tea and has become an important factor influencing consumer decisions. However, chemical formation mechanism chestnut-like during processing remains unclear. In this study, dynamic changes key components contributing to their precursors were analyzed in fresh leaves, fixation first baking tea.The thermal process had effect on volatile causing a significant decrease alcohols esters increase ketones, acids, phenols, sulfur...
Abstract Background The tea plant ( Camellia sinensis (L.) O. Kuntze) is one of the most economically important woody crops. Plastic greenhouse covering cultivation has been widely used in areas northern China. Chlorophyll not only crucial pigment for green tea, but also plays an role growth and development plants. Currently, little known about effect plastic on chlorophyll leaves. Results To investigate leaves, color difference values, contents, gene expression, enzyme activities...
Tea (Camellia sinensis) is an important commercial crop, in which the high content of flavonoids provides health benefits. A flavonoid glycosyltransferase (CsUGT73A20), belonging to cluster IIIa, was isolated from tea plant. The recombinant CsUGT73A20 Escherichia coli exhibited a broad substrate tolerance toward multiple flavonoids. Among them, kaempferol optimal compared quercetin, myricetin, naringenin, apigenin, and kaempferide. However, no product detected when UDP-galactose used as...
This study investigates the volatile compounds of green tea produced with different leaves from spring, summer, and autumn in high-latitude region. A total 95 were identified by gas chromatography-mass spectrometry (GC-MS). Spring, summer contained 68, 72 82 compounds, respectively. Principal component analysis (PCA), partial least squares-discrimination (PLS-DA), hierarchical cluster (HCA) classified samples showed difference. And 32 key characteristic components screened out based on...
Proanthocyanidins (PAs, also called condensed tannins), are an important class of secondary metabolites and exist widely in plants. Tea (Camellia sinensis) is rich PAs their precursors, (−)-epicatechin (EC) (+)-catechin (C). The biosynthesis constantly regulated by many different MBW complexes, consisting MYB transcription factors (TFs), basic-helix–loop–helix (bHLH) TFs, WD-repeat (WDR) proteins. These regulatory can be environmentally affected, such as biotic abiotic stresses. In this...
Abstract Benzoates, particularly salicylic acid (SA) and its derivatives, play critical roles in plant immune responses basal defense through hydroxylation glycosylation. Anthracnose is one of the most common devastating diseases tea plants (Camellia sinensis). However, role SA derivatives immunity resistance to anthracnose remains largely unexplored. In present study, we identified characterized a glycosyltransferase, CsUGT74B5, which was significantly downregulated seedlings upon...
Tea blending technology is based on finished tea. Blending fresh leaves during processing has not been proposed and investigated anywhere. This study investigates the impact of from different varieties flavor quality black The main taste components, including catechins, theaflavins, free amino acids, were analyzed using HPLC, while volatile components GC-MS. results show that adding Jinguanyin or Jinxuan to Fudingdabai can regulate ratio esterified non-esterified increase content theaflavins...
Anthocyanidin reductase (ANR) is a key enzyme in the ANR biosynthetic pathway of flavan-3-ols and proanthocyanidins (PAs) plants. Herein, we report characterization Shuchazao tea (Camellia sinesis), which an elite widely grown cultivar China rich providing with high nutritional value to human health. In our study, metabolic profiling was preformed identify two conjugates four aglycones flavan-3-ols: (−)-epigallocatechin-gallate [(−)-EGCG], (−)-epicatechin-gallate [(−)-ECG],...
Abstract It is still a serious challenge to develop simple synthesized method of SAPO‐34 sieve modified by Cu with high NH 3 ‐SCR performance. The Cu/SAPO‐34 samples hierarchical structure were for the first time using cupric citrate as copper source one‐pot hydrothermal which showed excellent performance NO removal. Among them, 0.15Cu/SAPO‐34 catalyst Cu/Al ratio 0.15 exhibited widest temperature operating window (160–500 °C) and better stability. physicochemical properties was measured...