Phenolic compounds in tea plant [Camellia sinensis (L.)] play a crucial role dominating flavor and possess number of key pharmacological benefits on human health. The present research aimed to study the profile tissue-specific, development-dependent accumulation pattern phenolic plant. A total 50 were identified qualitatively using liquid chromatography tandem mass spectrometry technology. Of which 29 quantified based their fragmentation behaviors. Most higher younger leaves than that stem...

Drying is an important factor in the formation of green tea flavor. In this study, metabolomic analysis were used to detect characteristic components four teas with clean aroma (CA), chestnut-like (CLA), bean-like (BLA) and high-fired (HFA) produced by adjusting drying temperature. The sensory evaluation results showed that dried at 90°C, 110°C, 140°C 160°C had a aroma, respectively. A total 95 volatile compounds identified. Linalool, naphthalene, 2-methyl-naphthalene, 1-octen-3-ol...

Tea [Camellia sinensis (L) O. Kuntze, Theaceae] is one of the most popular non-alcoholic beverages worldwide. Cold stress severe abiotic stresses that limit tea plants’ growth, survival and geographical distribution. However, genetic regulatory network signaling pathways involved in cold responses plants remain unearthed. Using RNA-Seq, DGE sRNA-Seq technologies, we performed an integrative analysis miRNA mRNA expression profiling their under chilling (4℃) freezing (-5℃) stress....

Green tea (Camellia sinensis, Cs) abundantly produces a diverse array of phenylpropanoid compounds benefiting human health. To date, the regulation biosynthesis in remains to be investigated. Here, we report cDNA isolated from leaf tissues, which encodes R2R3-MYB transcription factor. Amino acid sequence alignment and phylogenetic analysis indicate that it is member MYB4-subgroup named as CsMYB4a. Transcriptional metabolic analyses show expression profile CsMYB4a negatively correlated...

Salicylic acid (SA) is an important plant hormone and signal required for establishing resistance to diverse pathogens diseases. The abundant polyphenols in tea plants also defend from biotic abiotic stresses. However, whether exogenous SA would increase the of adversity relationship between are still poorly understood. Here, we carried out treatment on seedlings performed transcriptome sequencing. inhibited phenylpropanoid flavonoid metabolic pathways but promoted lignin pathways. increased...

Abstract Benzoates, particularly salicylic acid (SA) and its derivatives, play critical roles in plant immune responses basal defense through hydroxylation glycosylation. Anthracnose is one of the most common devastating diseases tea plants (Camellia sinensis). However, role SA derivatives immunity resistance to anthracnose remains largely unexplored. In present study, we identified characterized a glycosyltransferase, CsUGT74B5, which was significantly downregulated seedlings upon...

In field conditions, especially in arid and semi-arid areas, tea plants are often simultaneously exposed to various abiotic stresses such as cold drought, which have profound effects on leaf senescence process quality. However, most studies of gene expression stress responses focus a single inciting agent, the confounding effect multiple crop quality remain unearthed. Here, global transcriptome profiles leaves under separately drought were compared with their combination using RNA-Seq...

In the present study, proanthocyanidins were qualitatively and quantitatively identified using hydrolysis thiolysis assays, NP-HPLC, HPLC-ESI-MS, MALDI-TOF-MS, (1)H-NMR, (13)C-NMR techniques in different organs of tea plants. The results showed that leaves, tri-hydroxyl, cis- galloylated flavan-3-ols main monomeric catechins units, (epi)catechin was found to be major unit polymeric when degree polymerization greater than five. roots, PAs abundant, epicatechin formed predominant extension...

Melatonin and gibberellin are bioactive molecules in plants. In the present study, role of exogenous melatonin (MT) (GA) tea plant was explored by transcriptome metabolic analysis. Results showed that growth enhanced MT treatment. The pathways terpenoid synthesis plant-pathogen interaction were significantly strengthened, combined with upregulation LRR-RLK transcription factors which contributed to plant. internode elongation leaf enlargement hastened GA Significantly modulated expression...

A method using LC-ESI-IT-TOF/MS and LC/UV-ELSD was established to qualitatively analyze triterpene saponins obtained from the tea seed pomace (Camellia oleifera Abel). In addition, quantitative analysis of oleiferasaponin A1 LC/UV developed. The purified total did not exhibit any inhibitory effects at concentrations ranging 0.1 10 mg/mL against tested bacteria, except for Staphyloccocus aureus Escherichia coli. By contrast, higher activity seen fungi, especially Bipolaris maydis. Following...

Histochemical staining using vanillin−HCl is a potential tool to identify the site-specific accumulation of catechins in tea plant (Camellia sinensis (L.) O. Kuntze). Using this technique revealed that existed ubiquitously all inspected tissues young leaf, but distribution was concentrated vascular bundle and palisade tissue, whereas large parenchyma cells main vein contained lower amounts catechins. At subcellular level, were located mainly chloroplasts mesophyll vessel wall. In stems,...

One new and three known triterpenoid saponins were isolated identified from Camellia oleifera seeds through IR, NMR, HR-ESI-MS GC-MS spectroscopic methods, namely oleiferasaponin A₃, A₁, camelliasaponin B₁, B₂. The structure of A₃ was elucidated as 16α-hydroxy-21β-O-angeloyl-22α-O-cinnamoyl-23α-aldehyde-28-dihydroxymethylene-olean-12-ene-3β-O-[β-d-galactopyranosyl-(1→2)]-[β-d-xylopyranosyl-(1→2)-β-d-galactopyranosyl-(1→3)]-β-d-gluco-pyranosiduronic acid. Camelliasaponin B₁ B₂ exhibited...

Abstract Dalbergia odorifera T. Chen is a first-grade state protected plant in China. However, it difficult to distinguish from the closely related species tonkinensis Prain, which less important economic value, by wood anatomical features. In this study, three potential DNA barcode sequences, namely rpoC 1, trnH - psbA and internal transcribed spacer (ITS), were used differentiate of D. D . The average quantities extracts twigs, sapwood heartwood 16.3, 11.5 6.0 ng mg -1 , respectively....

Technology acceptance and usage become obligatory for people when their work modes change as a result of an unexpected but irresistible force. This is especially true teachers who are reluctant technology adopters compared with students. During the COVID-19 pandemic, Chinese government issued national policies to enforce online teaching learning. As success largely depends on university faculties' readiness intentions, how they perceive practice adoption becomes issue that warrants in-depth...

The tea plant, Camellia sinensis (L.) O. Kuntz, is a perennial woody plant widely cultivated for the production of popular non-alcoholic beverage. To rapidly identify and evaluate two different color varieties (yellowish purplish), main phenotypic traits quality components were tested in present study. metabolic profiles shoots leaves also analyzed using liquid chromatography-tandem mass spectrometry.The yellowish variation had higher active level with respect to metabolism catechins,...

Anthocyanidin reductase (ANR) is a key enzyme in the ANR biosynthetic pathway of flavan-3-ols and proanthocyanidins (PAs) plants. Herein, we report characterization Shuchazao tea (Camellia sinesis), which an elite widely grown cultivar China rich providing with high nutritional value to human health. In our study, metabolic profiling was preformed identify two conjugates four aglycones flavan-3-ols: (−)-epigallocatechin-gallate [(−)-EGCG], (−)-epicatechin-gallate [(−)-ECG],...

Tea tree [Camellia sinensis (L.) O. Kuntze] is an important leaf (sometimes tender stem)-using commercial plant with many medicinal uses. The development of newly sprouts would directly affect the yield and quality tea product, especially significant for Pingyang Tezaocha (PYTZ) which takes up a large percent in early spring market. MicroRNA (miRNA), particularly conserved miRNAs, often position center subtle complex gene regulatory systems, precisely control biological processes together...