A5072140565- Glycosylation and Glycoproteins Research
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Carbohydrate Chemistry and Synthesis
- Ubiquitin and proteasome pathways
- Supramolecular Self-Assembly in Materials
- Nanoparticle-Based Drug Delivery
- vaccines and immunoinformatics approaches
- Pancreatic function and diabetes
- RNA Interference and Gene Delivery
Genomics Research Center, Academia Sinica
2022-2025
Academia Sinica
2025
Significance To contain the spread of SARS-CoV-2, mRNA vaccine viral spike protein has proven to be highly effective, although efficacy against emerging variants is decreasing. Here, we show that with deletion glycosites in RBD or especially S2 domain expose more conserved epitopes can used as a broadly protective wild type and concern. The translated from such was not properly folded, thus induced cell apoptosis strong T response. Our findings demonstrate importance glycosylation effect on...
The globo-series glycosphingolipids (GSLs) are unique glycolipids exclusively expressed on the cell surface of various types cancer and have been used as targets for development vaccines therapeutics. A practical enzymatic method has developed synthesis glycans, where conversion Gb4 to Gb5 (SSEA-3) glycan based microbial galactosyltransferase LgtD is relatively inefficient compared other steps. To improve efficiency, we explored two human (β3GalT5) isozymes in cells this reaction found that...
Since the outbreak of Severe Acute Respiratory Syndrome Virus-2 (SARS-CoV-2) in 2019, more than 15 million spike protein sequences have been identified, raising a new challenge for development broadly protective vaccine against various emerging variants. We found that virus, like most other human viruses, depends on host-made glycans to shield conserved epitopes from immune response and demonstrated deletion glycan shields exposed highly elicited responses. In this study, we identified 17 14...
Human β1,3-galactosyltransferase 5 (β3GalT5) is a key enzyme involved in the synthesis of glycans on glycoproteins and glycolipids that are associated with various important biological functions, especially tumor malignancy cancer progression, has been considered as promising target for development anticancer agents. In this study, we determined X-ray structures β3GalT5 complex stable donor analogue UDP-2-fluorogalactose or native substrate UDP-galactose (UDP-Gal) several glycan acceptors at...
Aberrant glycosylation, especially sialylation, on cell surface is often associated with cancer progression and immunosuppression. Over-sialylation of stage-specific embryonic antigen-4 (SSEA-4) to generate disialylGb5 (DSGb5) was reported trigger Siglec-7 recognition suppress NK-mediated target killing. In this study, efficient chemo-enzymatic programmable one-pot methods were explored for the synthesis DSGb5 related sialosides assembly glycan microarrays evaluation binding specificity...
Abstract Nanoparticles, especially gold nanoparticles (GNPs) have emerged as promising tools for biomedical applications due to their unique properties and ability be functionalized. However, quantitative analysis of biomolecules conjugated remains a challenge. Here we report method conjugate synthetic hybrid‐type N ‐glycan GNPs quantitatively analyze the glycan content. The was first ‐hydroxysuccinimide (NHS)‐ester activated confirmed qualitatively by Fourier‐transform infrared spectroscopy...