A5007821586- SARS-CoV-2 and COVID-19 Research
- Experimental Learning in Engineering
- RNA Research and Splicing
- RNA modifications and cancer
- Silicon Carbide Semiconductor Technologies
- Cardiovascular Effects of Exercise
- COVID-19 Clinical Research Studies
- Melanoma and MAPK Pathways
- Computational Drug Discovery Methods
- Cardiac Structural Anomalies and Repair
- Engineering Education and Curriculum Development
- Long-Term Effects of COVID-19
- Cardiac tumors and thrombi
- Congenital heart defects research
- Engineering Education and Pedagogy
- Inflammasome and immune disorders
- Immune Response and Inflammation
- FOXO transcription factor regulation
- Mechanical Circulatory Support Devices
- Immune Cell Function and Interaction
Clemson University
2022-2024
Mississippi State University
2024
Cytokine release syndrome (CRS), commonly known as cytokine storm, is an acute systemic inflammatory response that a significant global health threat. Interleukin-6 (IL-6) and interleukin-1 (IL-1) are key pro-inflammatory cytokines involved in CRS hence critical therapeutic targets. Current antagonists, such tocilizumab anakinra, target IL-6R/IL-1R but have limitations due to their long half-life anti-inflammatory effects, making them less suitable for or localized treatments. Here we...
Human pluripotent stem cell (hPSC)-derived cardiac therapies hold great promise for heart regeneration but face major translational barriers due to allogeneic immune rejection. Here, we engineered hypoimmunogenic hPSCs using a two-step CRISPR-Cas9 strategy: (1) B2M knockout, eliminating HLA class I surface expression, and (2) knock-in of HLA-E or HLA-G trimer constructs in the AAVS1 safe harbor locus confer robust evasion. Hypoimmunogenic maintained pluripotency, efficiently differentiated...
Acute cardiac injuries occur in 20%–25% of hospitalized COVID-19 patients. Herein, we demonstrate that human organoids (hCOs) are a viable platform to model the caused by hyperinflammation. As IL-1β is an upstream cytokine and core signature cytokine, it was used stimulate hCOs induce release milieu proinflammatory cytokines mirror profile storm. The treated recapitulated transcriptomic, structural, functional signatures hearts. comparison with tissue from autopsies illustrated critical...
Trametinib is a MEK1 (mitogen-activated extracellular signal-related kinase 1) inhibitor used in the treatment of BRAF (rapid accelerated fibrosarcoma B-type)–mutated metastatic melanoma. Roughly 11% patients develop cardiomyopathy following long-term trametinib exposure. Although described clinically, molecular landscape cardiotoxicity has not been characterized. The aim this study was to test hypothesis that promotes widespread transcriptomic and cellular changes consistent with oxidative...
Cardiovascular disease (CVD) is the leading cause of death worldwide. To this end, human cardiac organoids (hCOs) have been developed for improved organotypic CVD modeling over conventional in vivo animal models. Utilizing cells, hCOs hold great promise to bridge key gaps research pertaining human-specific conditions. are multicellular 3D models which resemble heart structure and function. Varying fabrication techniques leads functional phenotypic differences. investigate heterogeneity...
Acute cardiac injuries occur in 20-25% of hospitalized COVID-19 patients. Despite urgent needs, there is a lack 3D organotypic models hearts for mechanistic studies and drug testing. Herein, we demonstrate that human organoids (hCOs) are viable platform to model the caused by hyperinflammation. As IL-1βis an upstream cytokine core signature cytokine, it was used stimulate hCOs induce release milieu proinflammatory cytokines mirror profile storm. The IL-1 β treated recapitulated...
Abstract Group work has become increasingly vital in engineering, as interdisciplinary collaboration commonplace many engineering workplaces. The education curriculum implemented universities mirrored this trend by introducing students to group-based cultivate group centric skills necessary for post-graduate work. A study was conducted within Mississippi State University's Department of Mechanical Engineering (ME) with a goal better understanding the experience ME undergraduate students....