A5084489139- Erythrocyte Function and Pathophysiology
- Glycosylation and Glycoproteins Research
- Neonatal Respiratory Health Research
- Carbohydrate Chemistry and Synthesis
- Lysosomal Storage Disorders Research
- Cancer Cells and Metastasis
- Immune Cell Function and Interaction
- Sphingolipid Metabolism and Signaling
- Cellular transport and secretion
- Hippo pathway signaling and YAP/TAZ
- SARS-CoV-2 and COVID-19 Research
- Retinal Development and Disorders
- Renal and related cancers
- Neuroblastoma Research and Treatments
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cell Adhesion Molecules Research
- Cancer, Hypoxia, and Metabolism
- Inhalation and Respiratory Drug Delivery
- Cancer Immunotherapy and Biomarkers
- Adipokines, Inflammation, and Metabolic Diseases
- Glaucoma and retinal disorders
- Congenital Diaphragmatic Hernia Studies
- Molecular Biology Techniques and Applications
- Chemical Synthesis and Analysis
- Wnt/β-catenin signaling in development and cancer
Kindai University
2015-2024
Tokai University
2007-2015
The complex-type N-linked octasaccharide oxazoline having LacNAc as the nonreducing end sugar was efficiently synthesized using benzyl-protected LacNAc, mannose, and β-mannosyl GlcNAc units key building blocks. To achieve a highly β-selective glycosylation with unit, N-trichloroacetyl group used for protection of amino in unit. After complete assembly these deprotection, obtained free successfully derivatized into corresponding oxazoline. On other hand, N-acetylglucosaminylated saposin C,...
<h3>Rationale</h3> Alveolar epithelial cell apoptosis and protease/antiprotease imbalance based proteolysis play central roles in the pathogenesis of pulmonary emphysema but molecular mechanisms underlying these two events are not yet clearly understood. Cell adhesion molecule 1 (CADM1) is a lung immunoglobulin superfamily. It generates membrane associated C terminal fragments (CTFs), αCTF βCTF, through protease mediated ectodomain shedding. <h3>Objective</h3> To explore hypothesis that more...
Somatic mutations in KRAS, NRAS, and BRAF genes are related to resistance anti-EGFR antibodies colorectal cancer. We have established an extended RAS mutation assay using a next-generation sequencer analyze these mutations. Multiplexed deep sequencing was performed detect somatic within BRAF, including minor mutated components. first validated the technical performance of multiplexed 10 normal DNA 20 formalin-fixed, paraffin-embedded (FFPE) tumor samples. To demonstrate potential clinical...
Saposins A, B, C, and D are small amphiphatic glycoproteins that encoded in tandem within a precursor protein (prosaposin, PSAP), required for vivo degradation of sphingolipids. Humans with saposin C deficiency exhibit the clinical presentation Gaucher-like disease. We generated two types mutant mice, one carrying homozygous missense mutation (C384S) domain prosaposin (Sap-C(-/-)) other compound heterozygous second null Psap allele (Psap(-/C384S)). During early life stages, both Sap-C(-/-)...
Although fibroblast growth factor (FGF) signals are strongly associated with malignancy, limited information is available regarding the role of FGF9 signal in colorectal cancer (CRC). In this study, we investigated frequency amplification CRC clinical specimens and association between gene resistance to anti-EGFR therapies. samples, an copy number gain >5 copies was observed at a 8/145 (5.5%) tended be related wild-type KRAS (7/96, 7.3%). Furthermore, not any samples from 15 responders...
Chronic kidney disease (CKD) is an important problem throughout the world, associated with increase of blood urea nitrogen (BUN) and serum creatinine (sCre) renal tubular injuries. It crucial to elucidate molecular mechanisms injuries identify new therapeutics early diagnostic methods. We focused on cell adhesion molecule-1 (CADM1) protein. CADM1, its isoform SP4, expressed in epithelial cells various tissues, including distal tubules, localized lateral membrane, mediates cell-cell via...
Optic nerve crush (ONC) induces retinal ganglion cell (RGC) death, which causes vision loss in glaucoma. To investigate early events leading to apoptosis of RGCs, we performed gene expression analysis injured retinas the period before RGC loss.The temporal changes profiles at 0, 1, and 4 days after ONC were determined by DNA microarray. verify enriched RGCs laser-captured microdissection real-time RT-PCR 14 selected genes. In situ localization study was immunohistochemistry.At 1 day ONC,...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) uses its S1 spike protein to bind angiotensin-converting enzyme (ACE2) on human cells in the first step of cell entry. Tryptanthrin, extracted from leaves indigo plant, Polygonum tinctorium, using d-limonene (17.3 µg/ml), is considered inhibit ACE2-mediated entry another type coronavirus, HCoV-NL63. The current study examined whether this extract could binding SARS-CoV-2 ACE2. Binding was quantified as cell-bound fluorescence...
Pulmonary emphysema and type 2 diabetes mellitus (T2DM), both caused by lifestyle factors, frequently concur. Respectively, the diseases affect lung alveolar pancreatic islet cells, which express cell adhesion molecule 1 (CADM1), an immunoglobulin superfamily member. Protease-mediated ectodomain shedding of full-length CADM1 produces C-terminal fragments (CTFs) with proapoptotic activity. In emphysematous lungs, rate thus level CTFs in cells increase. this study, expression was examined...
Elevation of intraocular pressure is a major risk factor for glaucoma development, which causes the loss retinal ganglion cells (RGCs). Lipocalin 2 (Lcn2) upregulated in glaucomatous retinae; however, whether Lcn2 directly involved debated. In this study, explant cultures were subjected to increased water using two-chamber culture device, and protein levels examined by immunoblotting. situ TdT-mediated dUTP nick labeling (TUNEL) glial fibrillary acidic (GFAP) immunohistochemical assays...
Lung alveolar epithelial cell (AEC) apoptosis has attracted attention as an early pathogenic event in the development of idiopathic interstitial pneumonia (IIP); however, causative mechanism remains unclear. Cell adhesion molecule 1 (CADM1) is AEC immunoglobulin superfamily. It generates a membrane-associated C-terminal fragment, αCTF, through protease-mediated ectodomain shedding, termed α-shedding. Increased CADM1 α-shedding contributes to emphysematous lungs. Formalin-fixed,...
Pulmonary emphysema is characterized histologically by destruction of alveolar walls and enlargement air spaces due to lung epithelial cell apoptosis. Cell adhesion molecule 1 (CADM1) an immunoglobulin superfamily member expressed in cells. CADM1 generates a membrane-associated C-terminal fragment, αCTF, through A disintegrin- metalloprotease-10-mediated ectodomain shedding, subsequently releasing the intracellular domain (ICD) γ-secretase-mediated intramembrane shedding αCTF. αCTF localizes...
Prosaposin is a precursor of saposins A, B, C, and D. Saposins are indispensable for lysosomal hydrolysis sphingolipids. The notion that prosaposin itself likely involved in brain development led us to generate an anti-mouse prosaposin-specific antibody do not cross-react with any the processed saposins. We have used it study expression wild-type (WT) saposin D knockout mice (Sap-D-/-). Immunoblot studies indicated prosaposin, already abundant WT, was dramatically increased Sap-D-/-. By...
Abstract Gene fusions play an important role in the carcinogenesis of lung adenocarcinoma. The recent association four oncogenic driver genes, ALK , ROS1 RET and NTRK1 as tumor predictive biomarkers has increased need for precision medicine. We used formalin‐fixed, paraffin‐embedded tissue samples non‐small cell cancer from 150 EGFR mutation‐negative cases 10 fusion status‐known compared performance Oncomine Dx Fusion Transcript Test (ODxFT) with FISH break‐apart detection genes. RNA was...
When epithelial cells in vivo are stimulated to proliferate, they crowd and often grow height. These processes likely implicate dynamic interactions among lateral membranous proteins, such as cell adhesion molecule 1 (CADM1), an immunoglobulin superfamily member. Pulmonary lines that express CADM1, named NCI-H441 RLE-6TN, were grown become overconfluent the polarized 2D culture system, examined for expression of CADM1. Western analyses showed CADM1 levels increased gradually up 3 times a...
We compared the performance of 3D-Gene® mutation assay (3D-Gene® KRAS kit) with Scorpion-ARMS (therascreen® RGQ PCR Kit) and Luminex (MEBGEN™ assays for detection mutations in formalin-fixed, paraffin-embedded tissue samples from 150 patients diagnosed colorectal cancer. DNA was extracted or without macrodissection under hematoxylin eosin staining status independently determined using these assays. Discordant results were re-analyzed by Sanger sequencing. Mutation analysis successfully...