A5062664948- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Renal and Vascular Pathologies
- Blood Pressure and Hypertension Studies
- Hormonal Regulation and Hypertension
- RNA Research and Splicing
- RNA regulation and disease
- DNA Repair Mechanisms
University of California, Berkeley
2021-2024
Stanford University
2024
Duke-NUS Medical School
2024
Palo Alto University
2022
QB3
2021
Clamp loaders are AAA+ ATPases that load sliding clamps onto DNA. We mapped the mutational sensitivity of T4 bacteriophage clamp and loader by deep mutagenesis, found residues not involved in catalysis or binding display remarkable tolerance to mutation. An exception is a glutamine residue module (Gln 118) located at catalytic interfacial site. Gln 118 forms hydrogen-bonded junction helical unit we term central coupler, because it connects centers DNA clamp. A suppressor mutation indicates...
Resistant hypertension affects 8.9% to 33% of all persons with hypertension, and is associated higher risks death, major cardiovascular events, left ventricular hypertrophy, progressive kidney disease, cognitive impairment. Guidelines recommend diagnostic work-up for resistant including assessment the renal vasculature but rates studies in real-world practice are not well known. From a cohort patients (n = 4660) from 2008-2016 prior founding Stanford Hypertension Center, we quantified...
ABSTRACT Clamp loaders are AAA+ ATPases that load sliding clamps onto DNA. We mapped the mutational sensitivity of T4 bacteriophage clamp and loader by deep mutagenesis, found residues not involved in catalysis or binding display remarkable tolerance to mutation. An exception is a glutamine residue module (Gln 118) located at catalytic interfacial site. Gln 118 forms hydrogen-bonded junction helical unit we term central coupler, because it connects centers DNA clamp. A suppressor mutation...