Single-nucleotide polymorphisms (SNPs) are the most abundant type of DNA sequence polymorphisms. Their higher availability and stability when compared to simple repeats (SSRs) provide enhanced possibilities for genetic breeding applications such as cultivar identification, construction maps, assessment diversity, detection genotype/phenotype associations, or marker-assisted breeding. In addition, efficiency these activities can be improved thanks ease with which SNP genotyping automated....

Rapid and consistent genotyping is an important requirement for cultivar identification in many crop species. Among them grapevine cultivars have been the subject of multiple studies given large number synonyms homonyms generated during centuries vegetative multiplication exchange. Simple sequence repeat (SSR) markers preferred until now because their high level polymorphism, codominant nature profile repeatability. However, rapid application partial or complete genome sequencing approaches...

The PipX factor is a regulatory protein that seems to occur only in cyanobacteria. In the filamentous, heterocyst-forming Anabaena sp. strain PCC 7120, open reading frame (ORF) asr0485, identified as pipX gene, expressed mainly under conditions of combined-nitrogen deprivation dependent on global N regulator NtcA and heterocyst-specific HetR. Primer extension 5' rapid amplification cDNA ends (RACE) analyses detected three transcription start points corresponding canonical NtcA-activated...

Temperature and solar radiation influence Vitis vinifera L. berry ripening. Both environmental conditions fluctuate cyclically on a daily period basis the strength of this fluctuation affects grape ripening too. Additionally, molecular circadian clock regulates cyclic expression in large proportion plant transcriptome modulating multiple developmental processes diverse organs phases. Circadian cycling fruit transcriptomes has not been characterized detail despite their putative relevance...

The association between polymorphism at the mc1r locus and colour variation was studied in two wall lizard species (Podarcis lilfordi P. pityusensis) from Balearic archipelago. Podarcis comprises several deep mitochondrial lineages, oldest of which originated Pliocene, while much shallower lineages are found pityusensis. Here, we examined whether specific substitutions were associated with melanic colouration islet populations these species. Homologous nuclear sequences covering most gene...

Abstract Two monophyletic sister species of wall lizards inhabit the two main groups B alearic I slands: P odarcis lilfordi from islets and small islands around M allorca enorca pityusensis biza, F ormentera associated islets. Genetic diversity within endangered . has been well characterized, but not studied in depth. Here, 2430 bp mt DNA 15 microsatellite loci were analysed populations across its natural range. genetic groupings identified, although geographical structuring differed...

AIMS: To measure Langerhans9 cells in skin of patients treated by bone marrow transplantation who developed chronic graft versus host disease (GvHD); to determine whether the reduction resulted directly from GvHD or other factors, such as immunosuppressive regimens used transplant patients. PATIENTS AND METHODS: Lesional and nonlesional specimens nine with lichen planus-like lesions three sclerodermoid were studied. Control taken undergoing breast surgery. The number cells/mm2 area a...

The calcium chloride chemical transformation of Escherichia coli is still the most widly used cloning method in small laboratories. Therefore, any practicable improvement its efficiency seems to be general interest.We found that giving competent cells a 1 min microwave pulse at lowest power setting (180 W), instead classic 1-2 42 degrees C heat-shock step, increases around threefold (3.3 +/- 0.5). Moreover, when both treatments were given 2-min - 5 on ice -1 sequence, an additional 1.6 was...

Podarcis tiliguerta is a wall lizard endemic to the Mediterranean islands of Corsica and Sardinia. Previous findings high mtDNA morphological diversity have led suggestion that it may represent species complex. Here, we analysed mitochondrial nuclear markers (mtDNA, 3110 bp; 6 nDNA loci, 3961 bp) in P. sampled from thirty-two localities across We find much greater intraspecific genetic divergence than between sister other island Podarcis, i.e., lilfordi pityusensis. detected three clusters...

ABSTRACT Humans now have a strong influence on almost every major aquatic ecosystem, and our activities dramatically altered the quality of receiving waters worldwide. Thus, there is continuous need to develop apply novel effective technologies detect, manage, correct human‐induced degradation systems. In present work, we evaluated molecular approach using polymerase chain reaction (PCR)‐temperature gradient gel electrophoresis (TGGE) measure changes in cyanobacterial diversity along...

Recently isolated populations offer a good biological model to infer the evolutionary forces responsible for current divergences across populations. We coupled genetic, morphometric, ecological, and demographic analyses from three island of endemic Balearic Wall Lizard, Podarcis lilfordi, (Balearic archipelago, Spain) mechanisms underlying observed differences in body size. For each population, we described plant community structure, derived biotic capacity index, used individual-based data...

Genetically, bipolar disorder is a complex genetic illness, in which both genes and environmental factors play an important role pathogenesis. Linkage studies have reported suggestive evidence for genomic regions, especially on chromosome 18, but most cases they been inconclusive. A total of 12 pedigrees, from the islands Majorca Minorca (Balearic Archipelago), with high expression mental studied. scan 29 polymorphic short tandem repeat markers was performed, spanning chromosomes 17 18 other...

titulo en ingles: Determination of adulterants in stockpiled crude milks from cheese processors Montería (Cordoba)Titulo portugues: Monteria (Córdoba)Resumen: La adulteración de un alimento es acto para degradar la calidad. Los solutos se diluyen y reduce su valor nutricional cuando le adiciona agua a leche. adición sólidos como féculas (almidones), sacarosa cloruros una práctica restablecer algunos parámetros fisicoquímicos enmascarar agua. El objetivo del presente estudio fue evaluar leche...

Antecedentes: La esclerosis múltiple es una enfermedad crónica, inflamatoria e incapacitante del siste- ma nervioso central. Afecta cualquier área funcional, principalmente a personas en edad productiva. rehabilitación debe ser integral involucrar los diferentes sistemas de manera simultánea. musicote- rapia neurológica ofrece estrategias las que se pueden estimular varias áreas cerebrales la vez. Objetivo: Analizar efectos musicoterapia con teclado aplicada paciente múlti- ple compromiso...

Objetivo: este estudio tuvo como objetivo describir la incidencia y analizar las principales características demográficas, epidemiológicas, clínicas, de diagnóstico tratamiento; así presencia factores riesgo pronósticos conocidos en pacientes costarricenses con e sclerosis múltiple, atendidos tres hospitales, que participaron el internacional denominado ENHANCE. Métodos: fue naturaleza observacional, se analizaron forma retrospectiva los registros médicos mayores 18 años diagnosticados...

The Ibizan wall lizard, Podarcis pityusensis, was the subject of several documented translocations by German vertebrate zoologist Martin Eisentraut, in 1930. He aimed to initiate long-term experiments into evolution melanism and other morphological traits accordingly he designed introductions five islets that (he believed) contained no lizards. In this study, we analyzed genetic characteristics individuals found there. We lizards on two islets, namely Escull de Tramuntana Galera, but for...