A5082611382- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Lanthanide and Transition Metal Complexes
- Enzyme Production and Characterization
- Crystal structures of chemical compounds
- Metal complexes synthesis and properties
- Crystallography and molecular interactions
- Enzyme Structure and Function
- Magnetism in coordination complexes
- Organometallic Compounds Synthesis and Characterization
- Microbial Metabolism and Applications
- Protein Structure and Dynamics
- Metal-Organic Frameworks: Synthesis and Applications
- RNA and protein synthesis mechanisms
- Carbohydrate Chemistry and Synthesis
- Yersinia bacterium, plague, ectoparasites research
- Monoclonal and Polyclonal Antibodies Research
- Quantum optics and atomic interactions
- Nanoplatforms for cancer theranostics
- Luminescence Properties of Advanced Materials
- Enzyme Catalysis and Immobilization
- Retinal and Optic Conditions
- Glycosylation and Glycoproteins Research
- Biochemical and Molecular Research
- DNA Repair Mechanisms
Charité - Universitätsmedizin Berlin
2022
Hospital Universitari de Girona Doctor Josep Trueta
2022
University of Girona
2022
Inserm
2022
Institut d'Investigació Biomèdica de Girona
2022
Jena University Hospital
2022
AB Science (France)
2022
Université de Lille
2022
Centre Hospitalier Universitaire de Lille
2022
Hospital Arnau de Vilanova
2022
Design of a new catalytic function in proteins, apart from its inherent practical value, is important for fundamental understanding enzymatic activity. Using computationally inexpensive, minimalistic approach that focuses on introducing single highly reactive residue into proteins to achieve catalysis we converted 74-residue-long C-terminal domain calmodulin an efficient esterase. The efficiency the resulting stereoselective, allosterically regulated catalyst, nicknamed AlleyCatE, higher...
Abstract The reaction of Salen‐like Zn II and Ni precursors with carbacylamidophosphate lanthanide moieties yields six new types 3d–4f compounds. complexes were characterized by means 1 H, 31 P NMR IR spectroscopy, elemental analysis, ESI mass spectrometry, X‐ray diffraction analysis. Depending on the Schiff base ligands, ion adopts either a square‐planar or an octahedral geometry, whereas has tetragonal‐pyramidal geometry. coordination number lanthanides is nine ten. Hydrolytic activities...
It only takes one mutation: A strategically placed single mutation in a non-enzymatic protein scaffold produced AlleyCat, small, allosterically regulated catalyst of Kemp elimination. In seven rounds directed evolution the enzymatic efficiency original 74 amino acid residue was improved more than 220-fold to achieve kcat value higher that catalytic antibodies for same reaction, still preserving allosteric regulation.
Abstract Lanthanide complexes with sulfonylamidophosphate‐type ligands: dibenzyl(phenylsulfonyl)amidophosphate (H SB ) and di(4‐methylphenyl)‐phenylsulfonyl‐amidophosphate SK have been synthesized show promise as new sensitizers of visible near‐infrared luminescence. They constitute a part coordination chemistry for this type ligand, creating simultaneously class lanthanide π ‐conjugated ligands possessing the structural fragment SO 2 NHP(O). Two series stable Na[Ln( 4 ] ( 1Ln 2Ln ), where...
We employed a minimalist approach for design of an allosterically controlled retroaldolase. Introduction single lysine residue into the nonenzymatic protein calmodulin led to 15,000-fold increase in second order rate constant retroaldol reaction with methodol as substrate. The resulting catalyst AlleyCatR is active enough subsequent directed evolution crude cell bacterial lysates. AlleyCatR's activity regulated by Ca(2+) ions. No catalysis observed absence metal ion. catalytic originates...
The crystal structure of the title compound, [NaNd(C8H11NO5PS)4]n, is composed two types crystallographically independent polymeric chains, A and B, respectively, which are formed by alternating anions sodium cations. In both NdIII ions eight-coordinated O atoms belonging to sulfonyl phosphoryl groups four bidentate chelate ligands. chain A, coordination polyhedron ion has a conformation intermediate between bicapped trigonal-prismatic square-antiprismatic, NaI coordinated N in distorted...
In the title compound, C(6)H(13)Cl(3)N(3)O(2)P or CCl(3)C(O)NHP(O)(N(CH(3))(2)), phosphinoyl group is synclinal to carbonyl and acts as an acceptor for inter-molecular N-H⋯O hydrogen bond from amide donor.
Nur eine Mutation genügt: Eine strategisch platzierte Einzelmutation in einem nichtenzymatischen Protein lieferte das Enzym AlleyCat, ein kleiner, allosterisch regulierter Katalysator der Kemp-Eliminierung. In nur sieben Runden gerichteter Evolution konnte die enzymatische Effizienz des ursprünglichen, 74 Aminosäuren langen Katalysators 220-fach gesteigert werden. Der kcat-Wert war damit höher als von katalytischen Antikörpern gleichen Reaktion, und allosterische Regulation wird noch dazu...
The crystal structure of the title compound, [U(NO(3))(2)O(2)(C(10)H(17)Cl(3)N(3)O(2)P)(2)], is composed centrosymmetric [UO(2)(L)(2)(NO(3))(2)] mol-ecules {L N-[bis-(pyrrolidin-1-yl)phosphor-yl]-2,2,2-trichloro-acetamide, C(10)H(17)Cl(3)N(3)O(2)P}. U(VI) ion, located on an inversion center, eight-coordinated with axial oxido ligands and six equatorial oxygen atoms phosphoryl nitrate groups in a slightly distorted hexa-gonal-bipyramidal geometry. One pyrrolidine fragments ligand disordered...
In the title complex, [Cu(C8H11NO5PS)(C18H15P)2], Cu(I) ion is coordinated by two tri-phenyl-phosphane mol-ecules and O atoms of chelating dimeth-yl(phenyl-sulfon-yl)amido-phosphate anion, generating a squashed CuO2P2 tetrahedron. six-membered chelate ring, Cu, P are almost coplanar (r.m.s. deviation = 0.024 Å), with N S displaced in same direction, 0.708 (5) 0.429 (2) Å, respectively.
In the title compound, [Ho(C6H12Cl3N3O2P)3(C18H15OP)], HoIII ion is surrounded by six O atoms from three bidentate N-[bis(dimethylamino)phosphinoyl]-2,2,2-trichloroacetamido ligands (L−) and one atom triphenylphosphine oxide ligand, with formation of a distorted monocapped octahedron. ligand L−, trichloromethyl group rotationally disordered between two orientations in 1:1 ratio, while dimethylamino groups another L− are conformations, each same ratio.
In the molecular structure of title compound, [NaNi(C18H18N2O4)(NO3)(CH3OH)], Ni(2+) ion has a slightly distorted square-planar coordination environment defined by two N and O atoms which belong to Schiff base ligand, viz. 6,6'-dimeth-oxy-2,2'-[ethane-1,2-diylbis(nitrilo-methanylyl-idene)]diphenolate. Seven form Na(+) ion: four from bidentate chelating nitrate anion one atom coordinating methanol mol-ecule. crystal, bimetallic complexes are assembled into chains along b-axis direction via...