A5009604367- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- SARS-CoV-2 and COVID-19 Research
- HIV Research and Treatment
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- CRISPR and Genetic Engineering
- HIV/AIDS drug development and treatment
- Respiratory viral infections research
- Mosquito-borne diseases and control
- Viral Infections and Immunology Research
- Quantum optics and atomic interactions
- Influenza Virus Research Studies
- Biopolymer Synthesis and Applications
- Cancer Research and Treatments
- Bacillus and Francisella bacterial research
- Herpesvirus Infections and Treatments
- Animal Virus Infections Studies
- Ferroelectric and Negative Capacitance Devices
- HIV/AIDS Research and Interventions
- Enterobacteriaceae and Cronobacter Research
- HIV/AIDS oral health manifestations
Shanghai Customs College
2020-2024
Wenzhou Medical University
2024
First Affiliated Hospital of Wenzhou Medical University
2024
Shenzhen International Travel Health Care Center
2019-2023
Heilongjiang University of Chinese Medicine
2023
Weatherford College
2023
Jining Medical University
2022
United States Customs and Border Protection
2020
Hunan Agricultural University
2019
Fudan University
2014
Viral evolution impacts diagnostic test performance through the emergence of variants with sequences affecting efficiency primer binding. Such that evade detection by nucleic acid-based tests are subject to selective pressure, enabling them spread more efficiently. Here, we report a variant-tolerant for SARS-CoV-2 using loop-mediated isothermal amplification (LAMP) assay containing high-fidelity DNA polymerase and polymerase-medicated probe (HFman probe). In addition demonstrating high...
Epigallocatechin gallate (EGCG), the most abundant catechin in green tea, has been reported to inhibit HIV-1 replication prior its integration into host DNA via various proposed mechanisms; however, specific main target(s) of EGCG remain unclear. In this study, we investigated a number these detailed mechanism(s) using cell-based model.Multinuclear activation galactosidase indicator assays were used for all experiments, including examination time addition and synergisms with nucleoside...
Abstract Coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 has a global pandemics. To facilitate the detection of infection, various RT-LAMP assays using 19 sets primers had been developed, but never compared. We performed comparative evaluation 4 RNA standards and 29 clinical samples from COVID-19 patients. Six 15 were firstly identified to have faster amplification when tested with four standards, further subjected parallel comparison remaining primer samples. Among these 10 sets,...
Abstract “Test‐and‐go” single‐nucleotide variation (SNV) detection within several minutes remains challenging, especially in low‐abundance samples, since existing methods face a trade‐off between sensitivity and testing speed. Sensitive usually relies on complex time‐consuming nucleic acid amplification or sequencing. Here, graphene field‐effect transistor (GFET) platform mediated by Argonaute protein that enables rapid, sensitive, specific SNV is developed. The provides nanoscale binding...
Several herbs are traditionally used in the treatment of a variety ailments particularly rural areas South Africa where herbal medicine is mainly source health care system. Many these have not been assessed for safety or toxicity to tissue organs mammalian recipients.
Objectives: To characterize the HIV subtypes prevalent among men who have sex with (MSM) in Beijing and to perform baseline genotypic analysis of anti-HIV drug resistance this population. Design: In 2005, half new HIV-1 infections occurred through unprotected China. MSM become second most vulnerable group infection. HIV-1-prevalent population as well their genetic biological characteristics not been defined. Methods: A cohort consisting 54 HIV-seropositive were recruited written informed...
Abstract Poly-γ-glutamic acid (γ-PGA) and nattokinase (NK) are the main substances produced by Bacillus subtilis natto in solid-state fermentation have wide application prospects. We found that our strains had higher activity of when soybeans were used as substrate to increase yield γ-PGA. Commercial production γ-PGA requires an understanding mechanism co-production. Here, we obtained maximum (358.5 g/kg, w/w) highest NK during analyzed transcriptome co-production NK. By comparing changes...
Integrated clustered regularly interspaced short palindromic repeat (CRISPR)-loop-mediated amplification (LAMP) technology is of great importance in CRISPR-based diagnostic systems, which urgently needs to be developed improve accuracy. A labor-free, contamination-free, and fully automated droplet manipulation platform for the CRISPR-LAMP has not been before. Herein, we propose a platform, can precisely manipulate perform combined reactions with high sensitivity specificity. SARS-CoV-2 Spike...
In human immunodeficiency virus (HIV) infection, not only HIV itself but also systemic immune activation plays a role in the disease progression to acquired deficiency syndrome (AIDS). The may be present even during highly active antiretroviral therapy (HAART). An increased expression of osteopontin, proinflammatory cytokine, HAART was reported lymph nodes infected individuals. Osteopontin is known involved pathogenesis various HAART-induced diseases. Here, we measured osteopontin and other...
Timely and accurate detection of SARS-CoV-2 variants concern (VOCs) is urgently needed for pandemic surveillance control. Great efforts have been made from a mass scientists in increasing the sensitivity operability, reducing turn-around time cost. Here, we report nucleic acid testing-based method aiming to detect discriminate mutations by combining RT-RPA CRISPR-Cas12a detecting assays (RRCd). With limit 10 copies RNA/reaction, RRCd was validated 194 clinical samples, showing 89% positive...
To study HIV-1 genetic diversity among HIV-positive men who have sex with (MSM) in China, a cohort consisting of MSM was established 2005 and monitored every 2 years. In 2007, 44 subjects were genotyped, the results showed subtype B decreased from 77.5% to 41.9%, but non-B subtypes increased rapidly represented by CRF01_AE 3.7% 30.2% compared 2005. addition, one case CRF07_BC first identified this population, which mainly circulated HIV-1-infected injection drug users (IDUs) China. There 11...
Since its first introduction into China in 2009, influenza A/H1N1pdm virus has undergone a rapid expansion and replaced the classical seasonal A(H1N1) virus. To characterize ongoing evolution national transmission dynamics of this virus, we analyzed 335 complete genome, 1259 HA, 1043 NA sequences strains detected China. We found that dN/dS value relative genetic diversity experienced decrease from 2009 to 2017, then increase during 2018-2019. Importantly, elevated was observed A/H3N2...
Background: A combination of pegylated interferon and ribavirin is the current standard therapy for hepatitis C virus (HCV) infection, but this provides relatively low efficacy, especially in some patients with HCV genotype 1 infection; therefore, development novel therapeutic agents required further improvement treatment chronic infection. Methods: pseudotype subgenomic replicon assays were used study. The interaction compounds receptors was examined using flow cytometry. Intracellular RNA...
Previous studies have highlighted CRISPR-based nucleic acid detection as rapid and sensitive diagnostic methods for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we reported an optimized CRISPR-Cas12a platform the safe of SARS-CoV-2 variants concern (VOCs). This platform, which was referred to CALIBURN-v2, could complete diagnosis on extracted RNA samples within 25 min in a closed-lid reaction mode had 100-fold increase sensitivity comparison with previous platforms....
Despite the need in various applications, accurate quantification of nucleic acids still remains a challenge. The widely-used qPCR has reduced accuracy at ultralow template concentration and is susceptible to nonspecific amplifications. more recently developed dPCR costly cannot handle high-concentration samples. We combine strengths by performing PCR silicon-based microfluidic chips demonstrate high large range. Importantly, low concentration, we observe on-site (osPCR), where only certain...
Vibrio parahaemolyticus is the predominant etiological agent of seafood-associated foodborne illnesses on a global scale. It essential to elucidate mechanisms by which this pathogen disseminates. Given existing research predominantly concentrates localized outbreaks, there pressing necessity for comprehensive investigation capture strains V. cross borders.
Abstract Coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 has a global pandemics. To facilitate the detection of infection, various RT-LAMP assays using 19 sets primers had been developed, but never compared. We performed comparative evaluation 4 RNA standards and 29 clinical samples from COVID-19 patients. Six 15 were firstly identified to have faster amplification when tested with four standards, further subjected parallel comparison remaining primer samples. Among these 10 sets,...
All four dengue virus serotypes covering nine genotypes were found to be imported into Shanghai, China, from overseas during 2017–19. Phylogenetic analyses showed that the majority of them Southeast Asian countries. These findings highlight importance strengthening surveillance in Shanghai.