A5050209439- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Animal Virus Infections Studies
- Mosquito-borne diseases and control
- Insect symbiosis and bacterial influences
- Viral gastroenteritis research and epidemiology
- Long-Term Effects of COVID-19
- COVID-19 Clinical Research Studies
- Vector-Borne Animal Diseases
- RNA Interference and Gene Delivery
- Animal Disease Management and Epidemiology
University of Bern
2022-2024
Federal Department of Home Affairs
2024
Institute of Virology of the Slovak Academy of Sciences
2023
Approved vaccines are effective against severe COVID-19, but broader immunity is needed new variants and transmission. Therefore, we developed genome-modified live-attenuated (LAV) by recoding the SARS-CoV-2 genome, including 'one-to-stop' (OTS) codons, disabling Nsp1 translational repression removing ORF6, 7ab 8 to boost host immune responses, as well spike polybasic cleavage site optimize safety profile. The resulting OTS-modified LAVs, designated OTS-206 OTS-228, genetically stable can be...
FIPV is of great significance in the cat population around world, causing 0.3%–1.4% feline deaths veterinary practices (2). As there are neither effective preventive measures nor approved treatment options available, an urgent need to identify antiviral drugs against FIPV. Our FCoV replicon system provides a valuable tool for drug discovery vitro . Due lack cell culture systems serotype I FCoVs (the most prevalent population) (2), different needed study these viruses. A viral studying FCoVs....
Abstract Japanese encephalitis virus (JEV) is a zoonotic mosquito-transmitted Flavivirus circulating in birds and pigs. In humans, JEV can cause severe viral with high mortality. Considering that vector-free direct transmission was observed pigs, introduction into an immunologically naïve pig population could result series of transmissions disrupting the alternating host cycling between vertebrates mosquitoes. To assess potential consequences such realistic scenario, we passaged ten times...
Japanese encephalitis virus (JEV) is a zoonotic mosquito-transmitted Flavivirus circulating in birds and pigs. In humans, JEV can cause severe viral with high mortality. Considering that vector-free direct transmission was observed experimentally infected pigs, introduction into an immunologically naïve pig population could result series of transmissions disrupting the alternating host cycling between vertebrates mosquitoes. To assess potential consequences such realistic scenario, we...
Bovine viral diarrhea virus (BVDV), a pestivirus in the family Flaviviridae, is major livestock pathogen. Horizontal transmission leads to acute transient infections via oronasal route, whereas vertical might lead birth of immunotolerant, persistently infected animals. In both cases, BVDV exerts an immunosuppressive effect, predisposing animals secondary infections. Erns, immunomodulatory protein, present on envelope and released as soluble protein. this form, it taken up by cells and, with...
The recurrence of zoonotic transmission events highlights the need for novel treatment strategies against emerging coronaviruses (CoVs), namely SARS-CoV, MERS-CoV and most notably SARS-CoV-2. Our recently performed genome-wide CRISPR knockout screen revealed a list conserved pan-coronavirus as well or HCoV-229E-specific host dependency factors (HDF) essential during viral life cycle. Intriguingly, we identified macroautophagy/autophagy pathway-regulating immunophilins FKBP8, TMEM41B, MINAR1...
<title>Abstract</title> Vaccines are the most effective measure against COVID-19. However, novel and highly efficacious vaccines with simplified administration broad immunogenicity, providing systemic mucosal immunity needed. Here, we show development of live-attenuated (LAV) based on (i) recoding SARS-CoV-2 genome to enrich for "one-to-stop" (OTS) codons, (ii) facilitating host responses by disabling non-structural-protein-1 (Nsp1) mediated translational repression, deletion open reading...