A5015707291- Muscle Physiology and Disorders
- Spaceflight effects on biology
- Renal and related cancers
- Adipokines, Inflammation, and Metabolic Diseases
- Muscle metabolism and nutrition
- Exercise and Physiological Responses
- Cell Adhesion Molecules Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Eicosanoids and Hypertension Pharmacology
- Adipose Tissue and Metabolism
- Eosinophilic Disorders and Syndromes
- Protein Tyrosine Phosphatases
- Genetics, Aging, and Longevity in Model Organisms
- Vitamin K Research Studies
- Ophthalmology and Eye Disorders
- Space Exploration and Technology
- Acute Kidney Injury Research
- Neurogenetic and Muscular Disorders Research
- Chronic Myeloid Leukemia Treatments
- High Altitude and Hypoxia
- Renal Diseases and Glomerulopathies
- Chronic Kidney Disease and Diabetes
- Histone Deacetylase Inhibitors Research
- Immune cells in cancer
- Peroxisome Proliferator-Activated Receptors
University of Tsukuba
2018-2024
Abstract Spaceflight causes a decrease in skeletal muscle mass and strength. We set two murine experimental groups orbit for 35 days aboard the International Space Station, under artificial earth-gravity (artificial 1 g ; AG) microgravity (μ MG), to investigate whether exposure prevents atrophy at molecular level. Our main findings indicated that AG onboard environment prevented changes soleus not only fiber type composition but also alteration of gene expression profiles. In particular,...
Abstract Skeletal muscle is sensitive to gravitational alterations. We recently developed a multiple artificial-gravity research system (MARS), which can generate gravity ranging from microgravity Earth (1 g ) in space. Using the MARS, we studied effects of three different levels (microgravity, lunar [1/6 ], and 1 on skeletal mass myofiber constitution mice. All mice survived returned Earth, was collected two days after landing. observed that microgravity-induced soleus atrophy prevented by...
Transcription factor MAFB regulates various homeostatic functions of macrophages. This study explores the role in brown adipose tissue (BAT) thermogenesis using macrophage-specific Mafb-deficient (Mafbf/f::LysM-Cre) mice. We find that Mafb deficiency macrophages reduces thermogenesis, energy expenditure, and sympathetic neuron (SN) density BAT under cold conditions. phenotype features a proinflammatory environment is characterized by macrophage/granulocyte accumulation, increases...
Abstract Muscle regeneration depends on muscle stem cell (MuSC) activity. Myogenic regulatory factors, including myoblast determination protein 1 (MyoD), regulate the fate transition of MuSCs. However, direct target MYOD in process is not completely clear. Using previously established MyoD knock-in (MyoD-KI) mice, we revealed that targets dual-specificity phosphatase (Dusp) 13 and Dusp27. In Dusp13:Dusp27 double knock-out ability for after injury was reduced. Moreover, single-cell RNA...
Abstract Monocytes and macrophages express the transcription factor MAFB (V-maf musculoaponeurotic fibrosarcoma oncogene homolog B) protect against ischemic acute kidney injury (AKI). However, mechanism through which alleviates AKI in remains unclear. In this study, we induced macrophage lineage-specific Mafb-deficient mice (C57BL/6J) using ischemia-reperfusion model to analyze these mechanisms. Our results showed that regulates expression of Alox15 (arachidonate 15-lipoxygenase) during AKI....
Long-duration spaceflight creates a variety of stresses due to the unique environment, which can lead compromised functioning skeletal and immune systems. However, mechanisms by organisms respond this stress remain unclear. The present study aimed investigate impact three different gravitational loadings (microgravity, 1/6 g [lunar gravity], 1 g) on behavior, bone, thymus, spleen mice housed for 25–35 days in International Space Station. bone density reduction under microgravity was mostly...
Multicentric carpotarsal osteolysis (MCTO) is a condition involving progressive of the carpal and tarsal bones that associated with glomerular sclerosis renal failure (MCTO nephropathy). Previous work identified an autosomal dominant missense mutation in transactivation domain transcription factor MAFB as cause MCTO. Several methods are currently used for MCTO nephropathy treatment, but these invasive lead to severe side effects, limiting their use. Therefore, development alternative...
Abstract Natto, known for its high vitamin K content, has been demonstrated to suppress atherosclerosis in large-scale clinical trials through a yet-unknown mechanism. In this study, we used previously reported mouse model, transplanting the bone marrow of mice expressing infra-red fluorescent protein (iRFP) into LDLR-deficient mice, allowing unique and non-invasive observation foam cells iRFP atherosclerotic lesions. Using 3 natto strains, meticulously examined effects varying levels on...
Abstract Muscle regeneration depends on muscle stem cell (MuSC) activity. Myogenic regulatory factors, including myoblast determination protein 1 (MyoD), regulate the fate transition of MuSCs. However, direct target MYOD in process is not completely clear. Using previously established MyoD knock-in (MyoD-KI) mice, we revealed that targets dual-specificity phosphatase (Dusp) 13 and Dusp27. In Dusp13 : Dusp27 double knock-out (DKO) ability for after injury was reduced. Moreover, single-cell...
Abstract Background and Aims MAFB is a podocyte-specific transcription factor. Point mutations in the transactivation domain humans result multicentric carpometacarpal-tarsal osteolysis (MCTO). MCTO patients show focal segmental glomerulosclerosis (FSGS) due to podocyte damage, known as nephropathy. Effective treatment for has not been found. Imatinib tyrosine kinase inhibitor (TKI), which inhibits PI3K-Akt pathway, long-term use of chronic myeloid leukemia. Method In order develop new MCTO,...