A5064149694- Cardiomyopathy and Myosin Studies
- Cardiac electrophysiology and arrhythmias
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Cellular transport and secretion
- Chemokine receptors and signaling
- Advanced Fluorescence Microscopy Techniques
- Cellular Mechanics and Interactions
- Erythropoietin and Anemia Treatment
- Mesenchymal stem cell research
- Osteoarthritis Treatment and Mechanisms
- Viral Infections and Immunology Research
- Ion channel regulation and function
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Iron Metabolism and Disorders
- Atrial Fibrillation Management and Outcomes
- Pluripotent Stem Cells Research
- Muscle Physiology and Disorders
- Cell Adhesion Molecules Research
- Signaling Pathways in Disease
Sechenov University
2024
University of Göttingen
2020-2024
German Centre for Cardiovascular Research
2020-2024
Universitätsmedizin Göttingen
2020-2024
Alterations in the buffering of intracellular Ca
Abstract The sarcomeric troponin-tropomyosin complex is a critical mediator of excitation-contraction coupling, stability and force generation. We previously reported that induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) from patients with dilated cardiomyopathy (DCM) mutation, troponin T (TnT)-R173W, display sarcomere protein misalignment impaired contractility. Yet it not known how TnT mutation causes dysfunction microdomains these events contribute to proteins in presence...
Abstract Dilated cardiomyopathy (DCM) is a major risk factor for heart failure and associated with the development of life-threatening cardiac arrhythmias. Using patient-specific induced pluripotent stem cell-derived cardiomyocyte (iPSC-CM) model harbouring mutation in troponin T (R173W), we aim to examine cellular basis arrhythmogenesis DCM patients this mutation. iPSC from control (Ctrl) DCM-TnT-R173W donors same family were differentiated into iPSC-CM analysed through optical action...
Joint-resident chondrogenic precursor cells have become a significant therapeutic option due to the lack of regenerative capacity in articular cartilage. Progenitor are located superficial zone cartilage, producing lubricin/Prg4 decrease friction cartilage surfaces during joint movement. Prg4-positive progenitors crucial maintaining joint’s structure and functionality. The disappearance progenitor leads changes hyaline over time, subchondral bone abnormalities, formation ectopic...
Systemic defects in intestinal iron absorption, circulation, and retention cause deficiency 50% of patients with heart failure. Defective subcellular uptake mechanisms that are independent systemic absorption incompletely understood. The main intracellular route for cardiomyocytes is clathrin-mediated endocytosis.We investigated patient-derived CRISPR/Cas-edited induced pluripotent stem cell-derived as well tissue. We used an integrated platform DIA-MA (mass spectrometry data-independent...
Intracellular cargo delivery via distinct transport routes relies on vesicle carriers. A key trafficking route distributes taken up by clathrin-mediated endocytosis (CME) early endosomes. The highly dynamic nature of the endosome network presents a challenge for its quantitative analysis, and theoretical modelling approaches can assist in elucidating organization system. Here, we introduce new computational approach assessment distributions. We employed model induced pluripotent stem...
Abstract We developed a new approach for combined analysis of calcium (Ca 2+ ) handling and beating forces in contractile cardiomyocytes. employed human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) from dilated cardiomyopathy (DCM) patients carrying an inherited mutation the sarcomeric protein troponin T (TnT), isogenic TnT-KO iPSC-CMs generated via CRISPR/Cas9 gene editing. In these cells, Ca as well –rates using single-cell atomic force microscopy (AFM) were assessed....
The restoration of hyaline cartilage poses a complex clinical and scientific challenge due to its low regenerative potential. Joint injuries contribute the development osteoarthritis and, as consequence, loss joint function, subsequent disability. Surgical approaches such mosaic chondroplasty microfracture are applicable only relatively small defects unsuitable for patients with degenerative conditions. Developing cell therapies using chondrocytes differentiated from induced pluripotent stem...