A5049758482- 3D Printing in Biomedical Research
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Cancer Immunotherapy and Biomarkers
- Advanced Biosensing Techniques and Applications
- Mitochondrial Function and Pathology
- Reproductive tract infections research
- Endoplasmic Reticulum Stress and Disease
- Immunotherapy and Immune Responses
- Hippo pathway signaling and YAP/TAZ
- Cell Adhesion Molecules Research
- S100 Proteins and Annexins
- Esophageal and GI Pathology
- Polymer Surface Interaction Studies
- Legume Nitrogen Fixing Symbiosis
- Cancer, Lipids, and Metabolism
- Cellular Mechanics and Interactions
- Tissue Engineering and Regenerative Medicine
- Cancer, Hypoxia, and Metabolism
- Lymphoma Diagnosis and Treatment
- Hydrogen's biological and therapeutic effects
- Wnt/β-catenin signaling in development and cancer
- Pancreatic function and diabetes
- Hydrogels: synthesis, properties, applications
- Lysosomal Storage Disorders Research
University of Nottingham
1999-2022
Queensland Health
2021-2022
Royal Brisbane and Women's Hospital
2019
Mater Research
2019
The University of Queensland
2019
Queen's Medical Centre
2009-2013
Royal College of Surgeons in Ireland
2004-2012
Dublin City University
2011
Implanted medical devices often elicit adverse foreign body responses whereby macrophages play a central role. Here, we identify simple polymers that instruct different immunological by modulating macrophage attachment and polarization to pro-inflammatory (M1-like) or anti-inflammatory (M2-like) phenotypes. These immune-instructive were discovered using in vitro high-throughput polymer microarray screening of diverse (meth)acrylate (meth)acrylamide libraries. The bioinstructive function is...
The ubiquitin-proteasome system (UPS) and macroautophagy (autophagy) are central to normal proteostasis interdependent in that autophagy is known compensate for the UPS alleviate ensuing proteotoxic stress impairs cell function. dysfunctions believed have a major role pathomechanisms of neurodegenerative disease. Here we show continued 26S proteasome dysfunction mouse brain cortical neurons causes paranuclear accumulation fragmented dysfunctional mitochondria, associated with earlier...
Wound healing is a complex biological process involving close crosstalk between various cell types. Dysregulation in any of these processes, such as diabetic wounds, results chronic nonhealing wounds. Fibroblasts are critical type involved the formation granulation tissue, essential for effective wound healing. 315 different polymer surfaces screened to identify candidates which actively drive fibroblasts toward either pro- or antiproliferative functional phenotypes. Fibroblast-instructive...
To metastasize, tumor cells must adopt different morphological responses to resist shear forces encountered in circulating blood and invade through basement membranes. The Rho Ras GTPases play a critical role regulating this dynamic behavior. Recently, we demonstrated shear-induced activation of adherent esophageal metastatic cells, characterized by formation membrane blebs. Although blebbing has only recently been as rounded mode cellular invasion promoted kinase (ROCK), the modulating...
Metastatic disease is dependent on tumor cell migration through the venous and lymphatic systems requires dynamic rearrangement of adherens junctions. Endocytosis cadherins a key mechanism to dynamically arrange junctions, signaling, motility in cells; however, role shear regulating this process metastatic cells unknown. In study, surface expression E-cadherin was investigated. We found that exposure (shear rate, 200/s) induced internalization adherent oesophageal (OC-1 line). Internalized...
Parkinson's disease (PD) is characterized by the progressive degeneration of substantia nigra pars compacta (SNpc) dopaminergic neurones and formation Lewy bodies (LB) in a proportion remaining neurones. α-synuclein main component LB, but pathological mechanisms that lead to neurodegeneration associated with LB remain unclear. Three pivotal elements have emerged development PD: α-synuclein, mitochondria protein degradation systems. We previously reported unique model, created conditional...
Neurodegenerative diseases are characterized by progressive degeneration of selective neurones in the nervous system, but underlying mechanisms involved neuroprotection and neurodegeneration remain unclear. Dysfunction ubiquitin proteasome system is one proposed hypotheses for cause progression neuronal loss. We have performed quantitative two-dimensional fluorescence difference in-gel electrophoresis combined with peptide mass fingerprinting to reveal proteome changes associated following...
Tumor-associated autoantibodies (AAbs) in individuals with cancer can precede clinical diagnosis by several months to years. The objective of this study was determine whether the primary immune response form IgM and gut mucosa-associated IgA aid IgG AAbs detection early-stage colorectal (CRC).
Interaction of tumor cells with the vascular wall is required for metastasis from bloodstream. The precise interaction among metastatic cells, circulating platelets, vessel wall, and physiological flow conditions remains to be determined. In this study, we investigated shear on cell lines adherent lipopolysaccharide (LPS)-treated endothelium. Tumor were perfused over LPS-treated human umbilical vein endothelial (HUVECs) at incremental venous rates 50 800 s(-1). At a rate 400 s(-1), 3% formed...
Metabolite profiling is an important tool that may better capture the multiple features of neurodegeneration. With considerable parallels between mouse and human metabolism, use metabolomics in models with neurodegenerative pathology provides mechanistic insight ready translation into aspects disease. Using 400 MHz nuclear magnetic resonance spectroscopy we have carried out a temporal region-specific investigation metabolome neuron-specific 26S proteasome knockout mice characterised by...
Hydroxylases are oxygen-sensing enzymes that regulate cellular responses to hypoxia. Transepithelial Cl− secretion, the driving force for fluid is dependent on O2 availability generation of energy. Here, we investigated role hydroxylases in regulating epithelial secretion and potential targeting these treatment diarrheal disorders. Ion transport was measured as short-circuit current changes across voltage-clamped monolayers T84 cells mouse colon. The antidiarrheal efficacy dimethyloxallyl...
The ATP binding cassette (ABC) proteins are a family of membrane transporters and regulatory responsible for diverse critical cellular process in all organisms. To date, there has been no attempt to investigate this class the infectious parasite Trichomonas vaginalis. We have utilized combination bioinformatics, gene sequence analysis, expression confocal microscopy ABC T. demonstrate that, uniquely among eukaryotes, vaginalis possesses intact full-length undergone dramatic expansion some...
Biochemical studies of plant auxin transporters in vivo are made difficult by the presence multiple and auxin‐interacting proteins. Furthermore, expression level most such plants is likely to be too low for purification downstream functional analysis. Heterologous systems should address both these issues. We have examined a number their efficiency expressing AUX1 from Arabidopsis thaliana . find that eukaryotic system based upon infection insect cells with recombinant baculovirus provides...
Crucial to organellogenesis was the development of membrane translocases responsible for delivering proteins new cellular compartments. This investigation examines Trichomonas vaginalis hydrogenosome, a mitochondrially derived organelle. We identify an expanded family putative β‐barrel (THOM A–I) comprising nine related sequences. Sub‐cellular localisation by immunofluorescence and biochemical fractionation is consistent with THOMs being localised hydrogenosomal membrane. Native gel...
As free-living organisms the ancestors of mitochondria and plastids encoded complete genomes, proteomes metabolomes. these symbionts became organelles all aspects were reduced - genomes have degenerated with host nucleus now encoding most remaining endosymbiont proteome, while metabolic processes symbiont been streamlined to functions emerging organelle. By contrast, topology membrane has preserved, necessitating development complex pathways for insertion translocation. In this study, we...
Abstract A coordinated mode of motility is required for dissemination and invasion esophageal metastases through the vasculature. This largely driven by activation GTPases Rac1 Rab5 which regulate 2 3-dimensional respectively; however, little known about localization these under hydrodynamic flow. In this study, effect on tumor was investigated in a controlled microfluidic chip that simulates venous flow conditions. metastatic cell line derived from ascites male patient with squamous cancer...
The presence of IgA- and IgM-specific autoantibody (AAb) isotypes their relationship to p53 tissue expression patterns are not well understood. This study aims investigate the clinical utility anti-p53 AAb positivity in colorectal cancer (CRC). We analysed IgG, IgM, IgA AAbs sera 99 CRC patients non-cancer control subjects. Corresponding protein was evaluated by immunohistochemistry (IHC). Anti-p53 IgG isotype were present 21 out (21%), while IgM observed 9 (9%) 2 (2%) patients. all three...
Conference Abstract| June 01 1999 MECHANISM OF REGULATION MICROSOMAL TRIGLYCERIDE TRANSFER PROTEIN GENE EXPRESSION BY DIETARY CHOLESTEROL Helen M. Sims; Sims 1Division of Nutritional Biochemistry, School Biological Sciences, University Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD Search for other works by this author on: This Site PubMed Google Scholar Andrew J. Bennett; Bennett *School Biomedical Nottingham Medical School, NG7 2UH Karen Lawler; Lawler Michael A. Billett;...
Conference Abstract| February 01 1999 REGULATION OF THE HEPATIC MICROSOMAL TRIGLYCERIDE TRANSFER PROTEIN(MTP) GENE BY DIETARY CHOLESTEROL IS MAINTAINED IN ISOLATED HEPATOCYTES Andrew J. Bennett; Bennett 1School of Biomedical Sciences, University Nottingham Medical School, Nottingham, NG7 2UH2Division Nutritional Biochemistry, School Biological Sutton Bonington Campus, Loughborough, LE12 5RD Search for other works by this author on: This Site PubMed Google Scholar Helen M. Sims; Sims Alan...