A5069569194- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- Glycosylation and Glycoproteins Research
- interferon and immune responses
- Cancer Immunotherapy and Biomarkers
- Electrohydrodynamics and Fluid Dynamics
- Cytokine Signaling Pathways and Interactions
- 3D Printing in Biomedical Research
- Ubiquitin and proteasome pathways
- Electrowetting and Microfluidic Technologies
- Advanced biosensing and bioanalysis techniques
- Innovative Microfluidic and Catalytic Techniques Innovation
- Immunotherapy and Immune Responses
- Biosensors and Analytical Detection
- Wnt/β-catenin signaling in development and cancer
- Cancer Research and Treatments
- Biomedical Text Mining and Ontologies
- Pickering emulsions and particle stabilization
- Nanopore and Nanochannel Transport Studies
- Protein purification and stability
- Pharmacological Effects of Natural Compounds
- Genetic factors in colorectal cancer
- Colorectal Cancer Treatments and Studies
Griffith University
2021-2024
The University of Queensland
2018-2022
Mater Research
2018-2022
Translational Research Institute
2018-2022
Dublin City University
2009-2017
Trinity College Dublin
2010-2011
Royal College of Surgeons in Ireland
2004-2009
Ruhr University Bochum
2004
Max Planck Institute for Molecular Genetics
2004
We present a novel centrifugal microfluidic platform for the highly efficient manipulation and analysis of particles applications in bead-based assays. The uses an array geometrical V-cup barriers to trap using stopped-flow sedimentation under reproducible hydrodynamic conditions. impact parameters governing occupancy distribution capture efficiency arrayed traps are investigated. unique, nearly 100% paired with capability establish sharply peaked, single distributions enables novel, digital...
In this work we present a centrifugal microfluidic system enabling highly efficient collective trapping and alignment of particles such as microbeads cells, their multi-colour fluorescent detection subsequent manipulation by optical tweezers.
Phase separation of heterogeneous liquids can occur in the presence sufficient shear force between a solid interface and liquid. We hypothesize that continuous flow oil through nanopores into water combination with given ultrasound energy generate stable free standing emulsions absence surfactants. An solution was pumped membrane 100 nm pores at controlled rate introduced an aqueous while being subjected to sonication. The resulting emulsion samples were collected specific time intervals...
Abstract We report the fabrication and characterisation of magnetic liquid beads with a solid shell core using microfluidic techniques. The consist fluorinated oil polymer magnetite particles. are generated in flow-focusing polydimethylsiloxane (PDMS) device cured by photo polymerisation. investigated response to an external field characterising their motion towards permanent magnet. Magnetic sorting channel was achieved 90% efficiency. results show that can be controlled magnetically have...
<h3>Objective:</h3> Patients with cancer have antibodies against tumour antigens. Characterising the antibody repertoire may provide insights into aberrant cellular mechanisms in development, ultimately leading to novel diagnostic or therapeutic targets. The aim of this study was characterise profiles patients whose symptoms warranted colonoscopy, see if there a difference and without colorectal cancer. <h3>Methods:</h3> were recruited from colonoscopy clinic. Individual serum samples 43 40...
Just as the Petri dish has been invaluable to evolution of biomedical science in last 100 years, microfluidic cell assay platforms have potential change significantly way modern biology and clinical are performed. However, an evolutionary process creating efficient array for many different bioassays is necessary. Specifically a complete view response it essential incorporate cytotoxic, protein gene analysis on single system. Here we present novel cellular molecular platform, which allows...
In medical diagnostics, detection of cells exhibiting specific phenotypes constitutes a paramount challenge. Detection technology must ensure efficient isolation (often rare) targets while eliminating nontarget background cells. Technologies exist for such investigations, but many require high levels expertise, expense, and multistep protocols. Increasing automation, miniaturization, availability technologies is an aim microfluidic lab-on-a-chip strategies. To this end, we present...
Abstract We present a substantially improved design and functionality of centrifugo‐magnetophoretic platform which integrates direct immunoseparation cost‐efficient, bright‐field detection cancer cells in whole blood. All liquid handling takes place disposable cartridge with geometry akin to conventional compact disc (CD). The instrumentation required process such “lab‐on‐a‐disc” can be as simple cost‐efficient the rotor on common optical drive. In first step, target blood sample are...
Colorectal cancer is one of the most common cancers worldwide with almost 700,000 deaths every year. Detection colorectal at an early stage significantly improves patient survival. Cancer-specific autoantibodies found in sera patients can be used for pre-symptomatic detection disease. In this study we assess zinc finger proteins ZNF346, ZNF638, ZNF700 and ZNF768 as capture antigens cancer. Sera from 96 35 control no evidence on colonoscopy were analysed presence ZNF-specific using indirect...
Ceramide synthase 5 is involved in the de novo synthesis of ceramide, a sphingolipid cell death and proliferation. In this study, we investigated role ceramide colorectal cancer by examining expression, clinico-pathological parameters association with survival/death signalling pathways cancer. Immunohistochemical analysis CerS5 was performed on 102 samples using tissue microarrays constructed from formalin-fixed paraffin-embedded tissues. We found strong membranous staining 57 (56%) cancers....
Automation is the key approach for genomewide and proteomewide screening of function interaction. Especially proteomics, antibody microarrays are a useful tool massive parallel profiling complex samples. To meet requirements to obtain great variety antibodies, new technologies such as phage display have partly replaced classical hybridoma method. While selection process phage-displayed fragments itself has been automated, bottleneck was shifted further downstream identification monoclonal...
Abstract Background and Aim TRIM 28 is a multi‐domain nuclear protein with pleotropic effects in both normal tumor cells. In this study, expression epithelial stromal microenvironment its prognostic role colorectal cancer were investigated. Methods Immunohistological staining of was evaluated tissue microarrays constructed from 137 patients. The correlations clinicopathological features p53 studied. K aplan– M eier analysis C ox proportional hazard modeling used to assess overall survival (...
This paper demonstrates the use of a transparent liquid marble as micro-photobioreactor for microalgal culture, with enhanced performance due to high light transmissivity and large surface area.
Tumor-associated autoantibodies (AAbs) in individuals with cancer can precede clinical diagnosis by several months to years. The objective of this study was determine whether the primary immune response form IgM and gut mucosa-associated IgA aid IgG AAbs detection early-stage colorectal (CRC).
Background & AimsChronic bowel inflammation increases the risk of colon cancer; colitis-associated cancer (CAC). Thiopurine treatments are associated with a reduction in dysplasia and CAC inflammatory disease (IBD). Abnormal Wnt/β-catenin signalling is characteristic >90% colorectal cancers. Immunosuppression by thiopurines via Rac1 GTPase, which also affects signalling. Autophagy implicated colonic tumors, topical delivery thiopurine thioguanine (TG) known to alleviate colitis augment...
The polymerase chain reaction (PCR) is a robust technique used to make multiple copies of segment DNA. However, the available PCR platforms require elaborate and time-consuming operations or costly instruments, hindering their application. Herein, we introduce sandwiched glass–polydimethylsiloxane (PDMS)–glass microchip containing an array reactors for real-time PCR-based detection waterborne bacteria. solution was loaded into in single step utilising capillary filling, eliminating need...